A kind of preparation method and application of Lilei goldenrod polysaccharide extract

A polysaccharide extraction and total polysaccharide technology, applied in the field of medicine, can solve the problems of no anti-fatigue effect, etc., and achieve the effect of prolonging the time of swimming with weight, obvious effect, and anti-fatigue effect

Active Publication Date: 2019-07-19
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on Lilei Jinxianlan, and there are no relevant reports on its anti-fatigue effect

Method used

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  • A kind of preparation method and application of Lilei goldenrod polysaccharide extract
  • A kind of preparation method and application of Lilei goldenrod polysaccharide extract
  • A kind of preparation method and application of Lilei goldenrod polysaccharide extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take 2 kg of Lilei Jinxianlan medicinal material, heat and reflux extraction with 12 times the weight of 80% ethanol for 3 times, each time for 2 hours, filter, dry the filter residue, add 12 times the weight of distilled water to the residue, heat and extract 3 times, each time 2 h, filter, combine the extracts, concentrate the extracts, the volume-to-mass ratio of the concentrated solution to the original medicinal material is 2:1, add 95% ethanol until the alcohol content is 80%, let stand for 12 h, centrifuge, and collect the precipitate , the precipitate was washed with 4 times the amount of absolute ethanol and acetone respectively, and then the precipitate was dried to obtain the Lilei chrysanthemum crude polysaccharide extract. Add water to dissolve the Lilei chinensis crude polysaccharide, and dialyze the supernatant with a dialysis bag with a relative molecular weight cut-off of 3500 or more for 48 hours, then pass the dialysate through a JK008 cation exchange ...

Embodiment 2

[0032] Take 2 kg of Lilei Jinxianlan medicinal material, heat and reflux extraction with 10 times the amount of 95% ethanol for 3 times, each time for 2 hours, filter, dry the filter residue, add 10 times the amount of distilled water to the residue, heat and extract 3 times, each time 2 h, filter, combine the extract, concentrate the extract, the volume mass ratio of the concentrate to the original medicinal material is 1:1, add 95% ethanol until the alcohol content is 80%, let it stand for 12 h, centrifuge, and collect the precipitate , wash the precipitate with 4 times the amount of absolute ethanol and acetone, and then dry the precipitate to obtain the Lilei chrysanthemum crude polysaccharide extract. Add water to dissolve the Lilei chinensis crude polysaccharide, and dialyze the supernatant with a dialysis bag with a relative molecular weight cut-off of 3,500 or more for 48 hours, then pass the dialysate through a 732-type cation exchange resin, and elute with 3 times the...

Embodiment 3

[0033] Example 3 Anti-fatigue experiment of the polysaccharide extract of Lilei goldenrod orchid (extract in Example 1)

[0034] Take male ICR mice, weighing 18-22g, and randomly divide them into blank control group, Rhodiola rosea extract group (positive drug group), low-dose Lilei goldenrod polysaccharide extract group, and Lilei goldenrod polysaccharide extract group. The medium-dose group and the Lilei goldenrod polysaccharide extract group had 8 mice in each group. The administration was continued for 14 days, and 1 hour after the last administration, the mice in each group were subjected to weight-bearing swimming exhaustion test. Each load 10% lead wire of its body weight on the root of the mouse tail is put into a glass tank with a water depth of 35 cm and a water temperature of (25 ± 0.5) °C to ensure that the mice cannot touch the bottom of the tank with their tails in the water. When the head of the mouse submerges in the water and cannot surface within 10 seconds,...

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Abstract

The invention relates to a preparation method for anoectochilus lylei rolfe ex downie polysaccharide extracts. The preparation method comprises the steps that after raw materials are subjected to degreasing, crude polysaccharide is obtained through water purification and alcohol precipitation, the crude polysaccharide is subjected to dialysis and ion exchange chromatography to remove protein and pigment, small molecule chemical compositions are removed, and the anoectochilus lylei rolfe ex downie polysaccharide extracts high in purity are obtained. Accordingly, the content of anoectochilus lylei rolfe ex downie polysaccharide in the extracts is on a glucose basis, and the content of polysaccharide accounts for 74% or above of the weight percentage of anoectochilus lylei rolfe ex downie extracts.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a preparation method and application of a polysaccharide extract of Lilei goldenrod with anti-fatigue effect. Background technique [0002] Fatigue is a common physiological phenomenon. Its process is that excessive labor or exercise causes changes in the body's physiological and biochemical functions, resulting in a temporary reduction in the body's labor or exercise capacity. In fact, fatigue is a protective response to prevent the body from failing. . Fatigue can lead to a series of consequences such as lack of energy, inattention, and decreased resistance, which can seriously affect work efficiency or sports performance. Therefore, it is of great significance to study measures to delay or eliminate fatigue. The chemical drugs currently on the market for anti-fatigue have certain effects in delaying fatigue and eliminating fatigue, but long-term use has certain addiction and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P39/00A61K36/898
CPCA61K31/715A61K36/898C08B37/006
Inventor 吴岩斌吴锦忠吴建国郑承剑易骏
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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