Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immunocyte culture medium, and culture method and application of immunocytes

A technology of immune cells and medium, which is applied in the field of cell culture, can solve the problems of slow cell expansion, achieve good immune cell function, and prolong the number of passages

Inactive Publication Date: 2015-08-05
BIOTOWNTEK CO LTD
View PDF8 Cites 56 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the deficiencies in the prior art above, the purpose of the present invention is to provide a culture medium, culture method and application of immune cells, aiming at solving the problem of cell expansion in the prior art. The problem of slow growth

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunocyte culture medium, and culture method and application of immunocytes
  • Immunocyte culture medium, and culture method and application of immunocytes
  • Immunocyte culture medium, and culture method and application of immunocytes

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0036] The composition of the immune cell culture medium (calculated in 1 liter) according to the present invention is shown in the following table:

[0037] Table 1. Basic medium composition

[0038] Element concentration or amount Ala (alanine) 0.05 mM Arg (arginine) 2.00 mM Asn (asparagine) 0.55 mM Asp (aspartic acid) 0.4 mM Cys (cysteine) 0.15 mM Glu (glutamic acid) 0.15 mM Gly (Glycine) 0.25 mM His (histidine) 0.15 mM Ile (Isoleucine) 2.50 mM Leu (leucine) 1.20 mM Lys (lysine) 1.00 mM Met (methionine) 0.60 mM Phe (phenylalanine) 1.05 mM Pro (proline) 0.02 mM Ser (serine) 0.70 mM Thr (threonine) 0.30 mM Trp (tryptophan) 0.10 mM Tyr (tyrosine) 0.15 mM Val (valine) 1.4 mM Gln (glutamine) 4 mM Insulin 2-10mg / L Transferrin (transferrin) 5-50mg / L Human Serum Albumin (HSA) 0.1g / L ascorbic acid 50mg / L...

Embodiment 1

[0042] Example 1. Accelerating effect on T cell proliferation

[0043] In order to check the influence of the culture medium described in the present invention on the expansion speed of T cell culture, the T cells of human origin are selected for use in vitro cell culture experiments, and the cells are pressed by 10 4 After inoculation, conventional culture medium (control group) and immune cell culture medium of the present invention (experimental group) were added for culture (37°C, 5% CO 2 ), and the cell density was measured by MTT method at 72 hours. The results of the study show that the expansion speed of T cells using the cell culture medium described in the present invention is compared with the conventional medium (Takara GT-T551), and the cell proliferation speed is increased by 3.8 times under the same conditions (see figure 2 ).

[0044] T cells cultured through the immune cell culture medium of the present invention ( figure 2 Middle solid column) T cells cu...

Embodiment 2

[0045] Example 2 Acceleration of NK cell proliferation

[0046] In order to check the influence of the immune cell culture medium of the present invention on the expansion speed of NK cell culture, select NK cells from mammalian organisms such as people, mice, rats to do in vitro cell culture experiments, and the cells are divided into 10 4 After inoculation, conventional culture medium (control group) and immune cell culture medium (experimental group) of the present invention were added to culture (37°C, 5% CO 2 ), and the cell density was measured by MTT assay after 72 hours. The research results show that, compared with the conventional culture medium (Takara GT-T551), the expansion speed of NK cells using the immune cell culture medium of the present invention is increased by 3.2 times under the same conditions (see image 3 ).

[0047] The NK cells (solid column) cultured by the immune medium of the present invention show a faster expansion rate than the NK cells (hol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an immunocyte culture medium, and a culture method and application of immunocytes. The immunocyte culture medium is composed of one or more selected from the group consisting of amino acids, vitamins, salts, lipids or polypeptide, and one or more selected from the group consisting of cytokines or antibodies; the immunocyte culture medium can quickly amplify different types of immunocytes, enables the amplification speed of the immunocytes to be increased by 3 to 5 times than the amplification speed of the immunocytes cultured with a conventional culture medium, and prolongs passage times; meanwhile, the immunocytes cultured by the immunocyte culture medium provided by the invention can maintain good immunocyte functions and exert effects including, but not limited to, anti-cancer effect and anti-aging effect, so the immunocytes have good application prospects.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to an immune cell culture medium, culture method and application. Background technique [0002] Many studies have shown that immune cells are not only an important natural defense system for the body to prevent foreign body infection, but also play an important role in the body's emergency response to fighting tumor cells. There are many types of immune cells, including but limited to T cells, B cells, NK cells, DC cells, and CIK cells. In recent years, many studies have shown that the rapid expansion of immune cells in an emergency state is the response of the immune response to environmental changes, thereby playing the role of phagocytosis, killing and removal of foreign substances. However, for cancerous tissues, tumor cells can have the characteristics of immune escape, that is, they can evade the normal immune defense system, and then grow excessively in the body to form tumor tis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/078C12N5/0783C12N5/0784
Inventor 刘小青
Owner BIOTOWNTEK CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com