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Uses of solanum nigrum extract in body fat reducing, weight reducing and obesity hepatitis treating

A technology of extract and body fat, which is applied in the application field of Solanum nigrum extract in reducing body fat, body weight and treating obese hepatitis, and can solve problems such as liver regeneration damage

Inactive Publication Date: 2015-08-05
CHUNG SHAN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fatty degeneration in the course of the disease and the true pathogenic mechanism of NASH derived from it are not yet clear, but a large number of hypotheses obtained from clinical experiments and animal experiments in recent years have shown that oxygen stress, insulin resistance and lipid Lipid peroxidation leads to a series of pathological cascading immune attack reactions such as diffuse fatty infiltration, inflammatory response, necrosis, apoptosis, regeneration damage, and activation of hepatic stellate cells in the liver.

Method used

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  • Uses of solanum nigrum extract in body fat reducing, weight reducing and obesity hepatitis treating
  • Uses of solanum nigrum extract in body fat reducing, weight reducing and obesity hepatitis treating
  • Uses of solanum nigrum extract in body fat reducing, weight reducing and obesity hepatitis treating

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Solanum nigrum (S. nigrum Linn.) is an annual herbaceous plant of the Solanaceae family Solanaceae, collected in the Miaoli mountainous area in central Taiwan. The preparation method of Solanum nigrum water extract (SWE) is to first wash the nightshade and soak it in water for 30 minutes. Heat to 100°C and continue heating for 40 minutes. The resulting water extract was filtered and concentrated, heated in a water bath at 90°C until it became milky, and then dried in an oven at 70°C.

[0030] SWE total polyphenol content analysis

[0031] Take gallic acid (5mg / mL) and dissolve it in methanol, and then make standards of different concentrations (50, 100, 250, 500 μg / mL) respectively, take 20 μL of standards of different concentrations and make up the volume to 1.6 mL with secondary water , then add 100 μL of 2N Folin-Ciocalteu phenol reagent, then add 300 μL of 20% Na2CO3 and mix well, react at 40°C for 40 minutes, measure the absorbance at 725nm, and treat a group at t...

Embodiment 2

[0044] Embodiment 2 animal test

[0045] A high-cholesterol diet induces a pattern of excess body fat accumulation in mice

[0046] After the mice acclimated to the environment for a week, they began to enter the experiment. The animals were randomly divided into four groups, control group (Group A), induction group (Group B), test group I (SWE0.5%, Group C) and test group II (SWE1%, Group D), with 10 animals in each group. Its detailed grouping is as follows: (Table 3)

[0047] Group A: control group, fed with standard formula feed;

[0048] Group B: induction group, fed with high fat diet (high fat diet, HFD);

[0049] Group C: HFD feed + SWE0.5%;

[0050] Group D: HFD feed + SWE1%.

[0051] Table 3 HFD feed formula composition of experimental animals

[0052]

[0053] ND: normal diet group; HFD: high-fat diet group.

[0054] Animal blood fat analysis

[0055] Before taking blood samples and liver tissue from animals, the principle is to fast the animals for 12-14...

Embodiment 3

[0066] Embodiment 3 mouse liver detection method

[0067] Changes in Lipid Distribution in the Liver

[0068] After the end of the experimental period, the mice were sacrificed and 0.5 g of liver tissue was removed, and the liver was extracted with 0.5 ml of hexane:isopropanol (3:2, v / v). The lipids in the cells were transferred to a glass centrifuge tube to air-dry naturally, and then redissolved in 200 μL of isopropanol to obtain an intracellular lipid extract, which was measured by commercially available enzyme assay reagents. Changes in lipid content, including total cholesterol, triglycerides.

[0069] liver lipid extraction

[0070] Take a quantitative amount of liver, add 20 times the mass of chloroform (chloroform) / methanol (2:1), homogenize with a homogenizer and filter with filter paper, take the clear liquid to remove the organic solvent with a vacuum concentrator, and then Redissolve with a mixed dose of chloroform / methanol (1:2), and place it in a preservation ...

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Abstract

The present invention provides uses of a composition containing a solanum nigrum extract in weight reducing and body fat reducing, wherein the composition is further used for treatment or prevention of obesity hepatitis.

Description

technical field [0001] The invention relates to a use of nightshade extract for reducing body fat and body weight and treating obese fatty liver disease. Background technique [0002] According to a survey conducted by the Taiwan Academic Foundation for Liver Disease Prevention and Treatment, it was found that fatty liver is a common liver problem among Taiwanese, with a prevalence rate as high as 40%. Although fatty liver will not cause immediate harm to life, it is an important warning sign for health. If it is not treated in time, it may lead to liver cirrhosis and liver cancer. The survey also showed that alcoholism, obesity and hyperlipidemia control are all risk factors for fatty liver. 76.3% of overweight people have fatty liver. In addition, the prevalence of fatty liver is also significantly correlated with obesity. [0003] The Liver Disease Foundation once conducted ultrasound examinations on 9,000 office workers, and found that the proportion of fatty liver was...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/81A61P3/04A61P3/06A61P1/16A61P39/06A61P29/00A23L1/30A61K127/00
Inventor 王朝钟
Owner CHUNG SHAN MEDICAL UNIVERSITY
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