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Method for extracting total flavonoids from hibiscus manihot

A technology of total flavonoids and cabbage hibiscus, which is applied in the field of natural product separation, can solve the problems of long processing time, large amount of organic solvent, and low extraction rate, so as to improve the extraction rate, improve product purity, and reduce the content of metal ions and inorganic salts Effect

Inactive Publication Date: 2015-08-05
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the disadvantages of low extraction rate, large amount of organic solvent, and long processing time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Dry and crush the cabbage hibiscus flower through a 60-100 mesh sieve, take 10 g of the cabbage hibiscus flower powder and put it into 40 mL of ethanol solution with a mass concentration of 80% to soak for 1 hour, then adjust the microwave power to 200W, use microwave extraction for 30 minutes, and filter to obtain the cabbage hibiscus flower Total flavonoid extract;

[0017] The hibiscus extract was first passed through a polysulfone hollow fiber membrane with a molecular weight cut-off of 30,000 Da, and the transmembrane pressure difference was 0.25 MPa to collect the permeate; The permeate was collected at 0.25 MPa; finally, it was concentrated through a polyimide nanofiltration membrane with a molecular weight cut-off of 200 Da, and the transmembrane pressure difference was 2.0 MPa, and the concentrated liquid was collected to obtain a concentrated liquid of total flavonoids of cabbage hibiscus;

[0018] Flow the concentrated solution of hibiscus cabbage through the...

Embodiment 2

[0020] Dry and pulverize the seeds of hibiscus cabbage through a 60-100 mesh sieve, take 10 g of hibiscus cabbage seed powder and soak in 40 mL of ethanol solution with a mass concentration of 60% for 3 hours, then adjust the microwave power to 400W, extract with microwaves for 20 minutes, and filter to obtain hibiscus cabbage Total flavonoid extract;

[0021] The hibiscus extract was first passed through a polysulfone hollow fiber membrane with a molecular weight cut-off of 40,000 Da, and the transmembrane pressure difference was 0.5 MPa to collect the permeate; The permeate was collected at 0.5MPa; finally, it was concentrated through a polyimide nanofiltration membrane with a molecular weight cut-off of 100Da, and the transmembrane pressure difference was 3.0MPa.

[0022] Flow the concentrated solution of hibiscus cabbage through the HPD400 macroporous adsorption resin column for adsorption for 2 hours, wash the macroporous resin column that has absorbed the concentrated so...

Embodiment 3

[0024] Dry and pulverize the stems of the hibiscus cabbage through a 60-100 mesh sieve, take 10 g of the stem powder of the hibiscus cabbage and put it into 200 mL of ethanol solution with a mass concentration of 40% to soak for 3 hours, then adjust the microwave power to 400 W, extract with microwaves for 40 minutes, and filter to obtain the hibiscus cabbage Total flavonoid extract;

[0025] The hibiscus extract was first passed through a polyethersulfone hollow fiber membrane with a molecular weight cut-off of 40000Da, and the transmembrane pressure difference was 0.5MPa to collect the permeate; then passed through a polyethersulfone hollow fiber ultrafiltration membrane with a molecular weight cut-off of 4000Da, The permeate is collected with a difference of 0.5 MPa; finally, it is concentrated through a cellulose acetate nanofiltration membrane with a molecular weight cut-off of 200 Da, and the transmembrane pressure difference is 3.0 MPa, and the concentrated liquid is col...

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Abstract

The invention discloses a method for extracting total flavonoids from hibiscus manihot. The method comprises the following steps: (1) extracting, wherein an ethanol solution having the mass concentration of 40-99% is used as an extraction solution and a hibiscus manihot total flavonoid extract is obtained by virtue of microwave extraction; (2) purifying, namely enabling the hibiscus manihot total flavonoid extract to sequentially pass through hollow fiber ultrafiltration membranes having the molecular weight cutoffs of 10000-50000Da and 2000-6000Da and collecting penetration liquid; next, concentrating the penetration liquid by use of a nanofiltration membrane having the molecular weight cutoff of 100-500Da, thereby obtaining a hibiscus manihot total flavonoid concentrated liquid; (3) refining, namely adsorbing the hibiscus manihot concentrated liquid by use of a macroporous adsorption resin, orderly performing water washing and alcohol washing, and performing freeze-drying to obtain the hibiscus manihot total flavonoids. Compared with the prior art, the technology of the method is capable of greatly increasing the hibiscus manihot total flavonoid extraction ratio, and also capable of reducing the content of macromolecular saccharides and protein in the product, reducing the content of metal ions and inorganic salt in the product and improving the product purity.

Description

technical field [0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for extracting total flavonoids from hibiscus hibiscus, in particular to a method for purifying total flavonoids in hibiscus hibiscus by membrane separation combined with a macroporous resin. Background technique [0002] Hibiscus manihot (Hibiscus manihot) is an annual herb of the genus Okra in the Malvaceae family. Cai Furong, also known as golden flower sunflower. The flowers, leaves, roots, stems and seeds of Hibiscus hibiscus are rich in flavonoids, especially the content of bioflavonoids in hibiscus cabbage is very high, which is nearly a hundred times higher than that of soybean and ginkgo, which are widely used in the production of flavonoids. The first known plant. Flavonoids have strong antioxidant activity, and they have significant effects in anti-cardiocerebral ischemia, hypoxia, enhancing antioxidant function in human metabolism, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185
Inventor 严峰胡常乐冷微王明霞许愿龙雪元
Owner TIANJIN POLYTECHNIC UNIV
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