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Magnetic fluorescent composite nanometer biological probe and preparation method thereof

A magnetic nanometer and magnetic nanoparticle technology, which is applied in the field of preparation of multifunctional nanocomposite microspheres, can solve the problems of reduced fluorescence intensity and shedding of quantum dots, achieve excellent magnetic properties and fluorescence properties, increase biological activity, and simple process. Effect

Inactive Publication Date: 2015-07-08
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of connecting quantum dots to magnetic nanoparticles through electrostatic attraction, due to the relatively weak electrostatic force, quantum dots are easy to fall off from the surface of magnetic nanoparticles, and at the same time, the combined quantum dots are exposed on the surface of composite nanoparticles. Susceptible to other factors such as the environment, resulting in reduced fluorescence intensity

Method used

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  • Magnetic fluorescent composite nanometer biological probe and preparation method thereof
  • Magnetic fluorescent composite nanometer biological probe and preparation method thereof
  • Magnetic fluorescent composite nanometer biological probe and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] 1.1 ZnSe quantum dots were prepared by the aqueous phase method. Under nitrogen protection, 0.01g NaBH 4 Dissolve 0.0061g of Se powder in 2 mL of distilled water, heat to 40°C, and obtain NaHSe solution after completely dissolving; take 0.0439 g of Zn(Ac) 2 Dissolve in 20 mL of distilled water, add 0.0737 g of reduced glutathione after it is completely dissolved, and adjust the pH to 11.5 with 1 mol / L NaOH solution to obtain a Zn precursor solution; transfer the Zn precursor into a three-necked flask NaHSe solution was quickly injected under the protection of nitrogen, and the oil bath was refluxed at 100°C. After magnetic stirring for 1 h, it was taken out and quickly cooled to room temperature in the refrigerator to obtain a colorless and transparent solution, which was the prepared ZnSe QDs solution.

[0040] 1.2 Preparation of Fe by hydrothermal method 3 o 4 nanoparticles. Weigh 3 g FeCl 3 Dissolve in 80 mL of ethylene glycol, stir and ultrasonically dissolve, ...

Embodiment 2

[0047] 2.1 Preparation of ZnSeZnS quantum dot solution. Firstly, ZnSe quantum dots were prepared by chemical co-precipitation method, and the preparation method was as described in the above-mentioned Example 1. Take 15 mL of prepared ZnSe quantum dots, in which the concentration of ZnSe quantum dots is 2.7×10 -3 mol / L, add 0.0138 g Zn(Ac) 2 , 0.0277 g reduced glutathione and 0.01 g thiourea, adjust the pH value to 10.5 with the prepared NaOH solution with a concentration of 1 mol / L, under magnetic stirring, reflux the oil bath at 100°C, and react for 2 h after magnetic stirring Put it into the refrigerator and quickly cool it to room temperature to obtain light yellow water-soluble ZnSeZnS quantum dots with high luminous efficiency.

[0048] 2.2 Preparation of Fe by hydrothermal method 3 o 4 nanoparticles. Take 2.78 g FeSO 4 ·7H 2 O, 4.32 g FeCl 3 ·6H 2 Dissolve O in 30 mL of distilled water, stir and dissolve with magnetic force, then add 30 mL of ethylene glycol, ...

Embodiment 3

[0055] 3.1 Preparation of CdTe quantum dots in aqueous phase. Under nitrogen protection, 0.0945g NaBH 4 Dissolve 0.0063g Te powder in 5 mL distilled water, heat to 40°C, and obtain NaHTe solution after completely dissolving; take 0.293 g Cd(Ac) 2 Dissolve in 100 mL of distilled water, add 2.0 mmol after it is completely dissolved, adjust the pH=11 with 1mol / L NaOH solution, and obtain the precursor solution of Cd; transfer the precursor of Cd into a three-neck flask, Quickly inject NaHTe solution, reflux in an oil bath at 100°C, magnetically stir for 6 hours, take it out and put it in the refrigerator to quickly cool to room temperature to obtain a colorless and transparent solution, which is the prepared CdTe QDs solution.

[0056] 3.2 Preparation of magnetic nanoparticles. Adopting the patent publication number to prepare Fe 3 o 4 / CoO core-shell magnetic nanoparticles.

[0057] 3.3 Carboxymethyl chitosan magnetic nanocomposites (CMCH-MNPs) were prepared by blending emb...

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Abstract

The invention relates to a preparation method of multifunctional nanometer composite microspheres and concretely relates to a magnetic fluorescent composite nanometer biological probe and a preparation method thereof. The nanometer composite microsphere is prepared by connecting carboxymethyl chitosan-modified magnetic nanoparticles and water soluble quantum dots through crosslinking effects of a cross-linking agent based on a natural organic polymer as a matrix, wherein the fluorescent quantum dots are distributed on the surface of the magnetic nanoparticle, the size of the magnetic nanoparticle is in a range of 10-200nm and the size of the quantum dot is in a range of 1.5-10nm, and after surface functional group modification, the nanometer composite particles can be connected to a biomolecule so that a bioprobe is formed. The preparation method allows mild reaction conditions and has simple processes. The prepared magnetic fluorescent composite nanometer particles have good magnetic properties and fluorescence properties and have a wide application prospect in the fields of biomarking, bioseparation, immunodetection, targeting release of drugs and target-oriented imaging.

Description

technical field [0001] The present invention relates to a method for preparing multifunctional nanocomposite microspheres, in particular to a magnetic fluorescent composite nanobiological probe and a method for preparing the same. The composite particle has both the fluorescent properties of quantum dots and the magnetic properties of magnetic nanoparticles. It can be used for targeting and bioluminescent imaging in vivo. Background technique [0002] Luminescent semiconductor quantum dots show great promise for biomarkers. Compared with organic luminescent materials, luminescent semiconductor quantum dots have a narrow emission spectrum, can realize tunable luminescence from ultraviolet, visible to infrared, and have a wide excitation band, and can be excited by a single wavelength of light to obtain emissions of different wavelengths. Light; at the same time, semiconductor quantum dots have good light stability and photobleaching resistance, and their inherent narrow emis...

Claims

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Application Information

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IPC IPC(8): C09K11/88B82Y30/00B82Y20/00B82Y25/00G01N21/64C07K1/13C07K1/14C12N15/10C12N9/00
Inventor 刘福田丁永玲孙康宁綦育仕闫君
Owner UNIV OF JINAN
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