Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A high-affinity anti-vomitoxin monoclonal antibody and its preparation method

A technology of monoclonal antibody and deoxynivalenol, which is applied in the field of immunoassay, can solve the problem of low affinity of anti-vomitin monoclonal antibody, and achieve the effects of overcoming low affinity, accurate detection, and cost reduction

Active Publication Date: 2018-01-19
SHANDONG LVDU BIO SICIENCE & TECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection methods of vomitoxin include chemiluminescence, liquid chromatography, enzyme-linked immunoassay, and immunocolloidal gold method. Among them, chemiluminescence and liquid chromatography require large-scale instruments and professionals, while other methods The core is to prepare high-affinity anti-vomitoxin monoclonal antibodies. At present, domestic anti-vomitoxin monoclonal antibodies have low affinity and are mostly imported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A high-affinity anti-vomitoxin monoclonal antibody and its preparation method
  • A high-affinity anti-vomitoxin monoclonal antibody and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation method of high-affinity anti-emetic toxin monoclonal antibody

[0027] (1) Deoxynivalenol complete antigen synthesis

[0028] Dissolve deoxynivalenol and N, N-carbonyldiimidazole (CDI) in pyridine at a mass volume ratio of 10:1 (mg:mL), and derivatize at 37°C for 3 hours to obtain the deoxynivalenol hapten. Add the hapten dropwise to Put into 0.01M PBS (3mL 0.01M PBS containing 20mg BSA) equivalent to 3 times the volume of pyridine, stir at room temperature for 1 hour, and dialyze with 0.01mol / LPBS for 2 days to obtain vomitoxin immune antigen. The synthesis process of vomitoxin complete antigen is as follows: figure 1 shown;

[0029] (2) Preparation of vomitoxin monoclonal antibody

[0030] ①Immunization program

[0031] The immune antigen obtained in step (1) was mixed with 501 adjuvant (Shandong Lvdu Biotechnology Co., Ltd., product number LD003) at a ratio of 1:1, and the 8-10-week-old Balb / c mice (purchased from Beijing Weitongli China Exp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a high-affinity anti-DON monoclonal antibody and a preparation method thereof. The preparation method includes the following steps: (1) complete antigen synthesis of DON; (2) preparation of DON monoclonal antibody. Step (2) includes immunization procedure, cell fusion, hybridoma screening and cloning, and antibody preparation and purification. The invention can quickly, sensitively and accurately detect the vomitoxin, and overcomes the problem that the domestic anti-vomitoxin monoclonal antibody has low affinity and is mostly imported.

Description

technical field [0001] The invention relates to a high-affinity anti-vomitoxin monoclonal antibody and a preparation method thereof, belonging to the technical field of immune detection. Background technique [0002] The toxin-producing bacteria of vomitoxin are mainly the dwarf fungus of the genus Fusarium, and vomitoxin mainly contaminates corn, wheat, rice, barley, millet and oats. Deoxynivalenol has an estrogen effect, mainly acting on the reproductive system, and can cause hyperestrogenism in livestock and poultry. Eating pasta made from vomitoxin-containing flour can cause central nervous system poisoning, with symptoms such as nausea, chills, headache, mental depression, and ataxia. Pregnant humans and animals eating food containing vomitoxin can easily cause miscarriage, stillbirth and teratogenicity. Therefore, it is of great significance to establish a rapid, sensitive and accurate detection technology for vomitoxin. [0003] At present, the detection methods of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/14G01N33/74G01N33/577C12N5/20
Inventor 武玉香沈志强王金良张莎莎于金枝
Owner SHANDONG LVDU BIO SICIENCE & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com