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Method for determining lignocelluloses component content of plant straw

A technology of lignocellulose and plant straw, applied in the field of agronomy, can solve the problems of misunderstanding, insufficient sample pretreatment, and inability to be universal.

Inactive Publication Date: 2015-05-27
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination of plant straw lignocellulose content is mainly divided into material pretreatment (chemical method, physical method, biological method and physical chemical method) and quantitative determination (gravimetric method, concentrated acid hydrolysis sugar determination method, ultraviolet spectrophotometry and isotope method). Marking method, etc.), the predecessors have carried out the determination of the lignocellulose content of corn, rice and other food crops, and biomass energy crops such as miscanthus, sugarcane, etc., because of insufficient sample pretreatment, extensive operation process, and later quantitative determination The method is imprecise or even erroneous, resulting in large errors in the final measurement results, and cannot be used universally in various plant straws

Method used

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  • Method for determining lignocelluloses component content of plant straw
  • Method for determining lignocelluloses component content of plant straw
  • Method for determining lignocelluloses component content of plant straw

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1: Accurate Determination of Lignocellulose Components in Miscanthus Straw

[0095] Miscanthus is a perennial high-efficiency C4 plant. It is considered to be the most potential non-grain cellulose energy plant in the world. The cellulose component can evaluate its biomass utilization value, evaluate the biological quality of different Miscanthus species, and tap the application potential of Miscanthus plants. Molecular mechanism research provides guidance.

[0096] 1) Material pretreatment

[0097] 1. Take materials

[0098] Time for collecting materials: the end of February or the beginning of March of the following year (preferably it has been sunny for several consecutive days)

[0099] Material collection equipment: fruit branch scissors, cotton gloves, rope about 30cm, mesh bag, material distribution map, signature pen, label paper

[0100] Material collection method: For species with relatively large single plants such as Nandi, Wujiemans, awns and hy...

Embodiment 2

[0218] Example 2: Accurate Determination of Lignocellulose Components of Corn Stalks

[0219] Corn is the world's largest food crop and an important feed crop. Corn stalks are important biomass energy sources. The lignocellulose components of corn stalks affect the conversion efficiency of biomass energy, the mechanical strength of straws and the lodging resistance of plants. We used this method to determine the lignocellulose components of corn stalks with different biological traits, including brittle corn, lodging corn and normal corn. This method was verified and the lignocellulose components of different types of corn materials were analyzed at the same time. Provide guidance for biomass production and lodging resistance breeding of maize.

[0220] 1) Material pretreatment

[0221] 1 draw

[0222] (1) Material collection period: corn growth and maturity period

[0223] (2) Part of the material: the third stem node of the aboveground part of the corn stalk

[0224] (3)...

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Abstract

The invention provides a method for determining the lignocelluloses component content of plant straw, and relates to the field of agronomy. The method particularly comprises the following steps: firstly, selecting the plant straw of a tested material for pretreatment; then carrying out two-step acidolysis respectively by adopting 72% concentrated sulfuric acid and 4% dilute sulfuric acid, and degrading and separating a lignocelluloses component in the plant straw; then accurately determining the content of cellulose and semi-cellulose by adopting an HPLC method, determining the content of acid-soluble lignin in a degraded liquid by adopting a UV method, and determining the content of acid-insoluble lignin by adopting a firing method. The method remedies the defects of insufficient pretreatment, extensive treatment process and inaccurate quantitative determination method caused by adopting a traditional method, gets a new breakthrough of determining the content of semi-fibers of lignin of the plant straw by adopting the HPLC method, can be used for accurately measuring the content of the lignocelluloses component in various plant straw, is accurate and reliable in result, and has wide reference and use values in the fields of agricultural crop production, biochemical analysis, biomass novel energy utilization and the like.

Description

technical field [0001] The invention relates to the field of agronomy, and specifically provides a method for measuring the content of lignocellulose components in plant straws. Background technique [0002] Plant straw lignocellulose mainly includes three components of cellulose (38%-50%), hemicellulose (23%-32%) and lignin (15%-30%) (Rubin, 2008), which constitute the The basic skeleton of the plant cell wall supports and protects the plant. The content of cellulose, hemicellulose and lignin in the plant straw is directly related to the mechanical strength of the plant, lodging resistance and other agronomic traits; the lignocellulose in the plant straw The components can be directly burned to generate heat, generate electricity, or be converted into ethanol, synthetic gas, and hydrogen after degradation (Xie Guanghui et al., 2007). It is a renewable resource with abundant sources that can be used to produce alternative energy (Himmel et al., 2007), the content of cellulo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N21/33G01N5/04
Inventor 陈翠霞贾生风赵东波葛春霞
Owner SHANDONG AGRICULTURAL UNIVERSITY
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