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Graphene/graphene oxide microarray electrode as well as preparation method and application thereof

A microarray electrode, graphene technology, applied in electrochemical variables of materials, material analysis by electromagnetic means, measurement devices, etc., can solve the problems of high catalytic activity, low response time, etc., and achieve improved sensitivity and high repeatability. , the preparation method is simple and easy to implement

Active Publication Date: 2015-05-27
安徽中科大禹科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Studies have shown that graphene has a certain electrocatalytic oxidation effect on hydrogen peroxide, but its catalytic activity is not as high as that of metal catalysts
Microarray sensors have the advantages of high sensitivity, signal-to-noise ratio, and low response time. Therefore, the development of graphene / graphene oxide microarray biosensors not only improves the catalytic activity, but also maintains the biocompatibility of the electrodes. detection is important, and this design has not been reported so far

Method used

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  • Graphene/graphene oxide microarray electrode as well as preparation method and application thereof
  • Graphene/graphene oxide microarray electrode as well as preparation method and application thereof
  • Graphene/graphene oxide microarray electrode as well as preparation method and application thereof

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Effect test

Embodiment 1

[0026] Embodiment 1: This embodiment prepares graphene / graphene oxide microarray electrode, comprises the following steps:

[0027] (1) Cleaning of ITO glass: put a 6mm×9mm ITO glass in a beaker, use acetone, absolute ethanol, and deionized water to sonicate for 10 minutes, and then dry it with nitrogen gas for use.

[0028] (2) Design a lithography template, in which there are graphics arranged in an orderly manner, and these graphics can be circular, such as figure 2 As shown, it can also be a square, a triangle, a hexagon, etc. In this embodiment, a commonly used circular pattern is used, and the template is prepared by the Institute of Microelectronics, Chinese Academy of Sciences, Beijing.

[0029] (3) Use photolithography technology to carve array pattern on ITO glass: specifically: Spin-coat positive photoresist on ITO glass, coating speed is 5000rpm, spin-coating time is 10s; after spin-coating, ITO glass Bake at 100°C for 90s to obtain a photoresist layer; then plac...

Embodiment 2

[0033] Embodiment 2: The graphene / graphene oxide array electrode prepared in embodiment 1 is used for the mensuration of hydrogen peroxide concentration.

[0034] Figure 5 Graphene / graphene oxide arrays with different reduction times (eg image 3 Shown) in the catalytic current density curve of 10mM hydrogen peroxide, it can be seen from the figure that the electrocatalytic hydrogen peroxide decomposition ability of the sample I-4 reduced for 30s is the strongest, so this embodiment uses the sample I-4 to measure the unknown hydrogen peroxide concentration.

[0035] Use sample I-4 as the working electrode, the silver / silver chloride electrode as the reference electrode, and the platinum sheet electrode as the counter electrode to form a three-electrode system; when measuring the concentration of hydrogen peroxide, place the three-electrode system in a 30ml Na 2 HPO 4 solution and a concentration of 0.1M NaH 2 PO 4 The solution was placed in a beaker of a mixed solution ...

Embodiment 3

[0036] Example 3: The graphene / graphene oxide microarray electrode I-4 prepared in Example 1 was used for the cultivation of nerve cells and the determination of hydrogen peroxide secreted by cells.

[0037] After sterilizing sample I-4, inoculate nerve cell PC12 at a density of 3000 cells / mL, and culture in a medium containing 10% fetal bovine serum and 1% double antibody for 24 hours. The growth state of the cells was observed by immunofluorescent double-staining of the actin and the nucleus of the cells, wherein, the F-actin protein of the cells was stained with Acti-Stain Phalloidin, and the nucleus was stained with DAPI. In the detection of hydrogen peroxide secreted by cells, the graphene / graphene oxide array was inoculated and cultured for 24 hours as the working electrode, the silver / silver chloride electrode was used as the reference electrode, and the platinum sheet electrode was used as the counter electrode to form a three-electrode system. Concentration of 0.2M Na...

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Abstract

The invention discloses a preparation method of a graphene / graphene oxide microarray and application of the graphene / graphene oxide microarray used as a chemical and cell-based sensor. A graphene / graphene oxide microarray electrode is prepared on an ITO glass substrate by combining a micro processing technology and an electrochemical method. By utilizing the electrochemical catalytic oxidation of the microelectrode prepared by the invention on hydrogen peroxide, the in vitro and intravital quantitative analysis and detection of hydrogen peroxide are realized by a timing amperometry. The preparation method is simple and feasible, the sensitivity is high, the response time is short, the biocompatibility is good, and the concentration of hydrogen peroxide can be quickly detected.

Description

technical field [0001] The invention belongs to the field of microprocessing and biological detection, and relates to a graphene / graphene oxide microarray electrode and a preparation method and application thereof. Background technique [0002] Hydrogen peroxide is the ubiquitous and most stable reactive oxygen species (ROS) in cells, which plays an important role in cellular DNA damage, apoptosis, and protein synthesis. Studies have shown that there is a certain relationship between the abnormal concentration of hydrogen peroxide in cells and diseases such as Alzheimer's disease, Parkinson's syndrome, arteriosclerosis, and cancer. Detecting the concentration of hydrogen peroxide secreted by cells is of great significance for the research of disease pathology, disease diagnosis and prevention. At present, electrochemical sensors for hydrogen peroxide are mainly divided into two types: enzymes and non-enzymes. Enzyme sensors are expensive and have strict usage conditions; n...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/413
Inventor 刘爱萍赵明徐盼举
Owner 安徽中科大禹科技有限公司
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