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Preparation method of low-molecular weight chitosan dressing

A low-molecular-weight, chitosan technology, used in medical science, bandages, absorbent pads, etc., to achieve the effect of reducing the amount of use, good antibacterial, and reducing the degree of pollution

Active Publication Date: 2015-05-20
GUANGDONG TAIBAO MEDICAL SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of preparing chitosan into powder antibacterial and hemostatic dressings has not been reported at present

Method used

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  • Preparation method of low-molecular weight chitosan dressing
  • Preparation method of low-molecular weight chitosan dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) After thawing Antarctic krill at 4°C, mash the whole shrimp with a tissue masher, weigh 100 g of the mashed tissue fluid and put it into a fermenter, inoculate the fermenter with a volume fraction of 10% activated After the lactic acid bacteria, the activated medium is MRS medium, the initial pH of fermentation is 6.0, the fermentation temperature is 37°C, and the fermentation time is 48 h. Impurities such as protein, calcium lactate, astaxanthin, rinse until colorless, and then dry the filter residue. Lactic acid bacteria can produce a large amount of lactic acid in the fermentation tank, and use the lactic acid produced during the fermentation process and endogenous protease to remove minerals and proteins in Antarctic krill without adding acidity and alkalinity such as hydrochloric acid, acetic acid, citric acid, and sodium hydroxide Substance that demineralizes and proteins from Antarctic krill.

[0020] (2) Mix the filter residue obtained in step (1) and NaOH ...

Embodiment 2

[0025] (1) After thawing Antarctic krill at 4°C, mash the whole shrimp with a tissue masher, weigh 100 g of the mashed tissue fluid and put it into a fermenter, inoculate the fermenter with a volume fraction of 10% activated After the lactic acid bacteria, the activated medium is MRS medium, the initial pH of the fermentation is 7.5, the fermentation temperature is 45°C, and the fermentation time is 96 h. Impurities such as protein, calcium lactate, astaxanthin, rinse until colorless, and then dry the filter residue.

[0026] (2) Mix the filter residue obtained in step (1) and NaOH solution with an alkali mass concentration of 25% at a mass volume ratio of W / V=1:15, and then ultrasonically treat it at 80°C for 12 h.

[0027] (3) After the ultrasonication is over, adjust the mixed solution to neutrality with an acidic solution of 4 mol / L HCl solution, then repeatedly rinse with distilled water several times, and dissolve the washed solids in acetic acid with a volume concentrat...

Embodiment 3

[0031] test group:

[0032] (1) After thawing Antarctic krill at 4°C, mash the whole shrimp with a tissue masher, weigh 100 g of the mashed tissue fluid and put it into a fermenter, inoculate the fermenter with a volume fraction of 10% activated After the lactic acid bacteria, the activated medium is MRS medium, the initial pH of fermentation is 7.0, the fermentation temperature is 42°C, and the fermentation time is 72 h. Impurities such as protein, calcium lactate, astaxanthin, rinse until colorless, and then dry the filter residue.

[0033] (2) Mix the filter residue obtained in step (1) and NaOH solution with a mass concentration of 20% at a mass volume ratio of W / V=1:10, and then ultrasonically treat it at 65 °C for 8 h.

[0034] (3) After ultrasonication, adjust the mixed solution to neutral with 4 mol / L HCl solution, then rinse repeatedly with distilled water for several times, dissolve the washed solid in 1% acetic acid by volume, filter Remove the insoluble matter, c...

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Abstract

The invention belongs to the field of medical chitosan dressings, and in particular relates to a preparation method of a low-molecular weight chitosan dressing. The preparation method of the low-molecular weight chitosan dressing comprises the following steps: (1) by using euphausia superba as raw materials, and preparing chitin through a lactic acid bacteria fermenting method; (2) removing acetyl on the chitin through the combination of alkali liquor and ultrasonic technology to prepare the chitosan; (3) purifying the acetic acid to extract the low-molecular weight chitosan; and (4) preparing the low-molecular weight chitosan dressing by using the low-molecular weight chitosan powder. Through the adoption of the preparation method disclosed by the invention, the pollution of the acid-base to the environment is greatly reduced, and the low-molecular weight chitosan prepared by using the euphausia superba through the microorganism fermentation and ultrasonic technology and the dressing thereof have good antibacterial and hemostatic effect, the allergic reaction is reduced, and the dressing can be sprayed to effectively achieve the hemostatic effect when the wound is bruised and massive in bleeding.

Description

technical field [0001] The invention belongs to the field of medical chitosan dressings, in particular to a preparation method of low molecular weight chitosan dressings. Background technique [0002] Chitosan has a wide range of applications in chemical, food, medical and other industries. Chitosan is the deacetylation product of chitin, which is very abundant in nature and widely exists in the shells of shrimps, crabs and insects, as well as in the cell walls of algae and fungi. It is a natural polymer and the only natural alkaline polysaccharide found so far. The molecular weight of chitosan usually ranges from hundreds of thousands to several million. Because of the strong hydrogen bond in the molecule, it can only be dissolved in dilute acid solution, but not directly in water, resulting in limited range of use. Big restrictions. [0003] At present, there are many researches on the preparation methods of low molecular weight chitosan in various countries in the worl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26A61L15/28A61L15/46
Inventor 卢亢陈泽楚李婷陈锦涛
Owner GUANGDONG TAIBAO MEDICAL SCI TECH
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