Traditional Chinese medicine preservative for fish stripping specimen and preparation method of traditional Chinese medicine preservative
A technology of preservatives and traditional Chinese medicines, applied in applications, preservation of human or animal bodies, animal husbandry, etc., can solve the problems of inconsistent ideas, high prices, waste of resources, manpower and material resources, etc., and achieve a good broad-spectrum bactericidal effect, preparation The method is simple and the antibacterial effect is good
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Embodiment 1
[0029] (1) Wash five kinds of traditional Chinese medicines such as rhubarb, coptis, astragalus, galangal, and forsythia, and dry them in an oven (60°C, 48 hours) respectively, and crush them into powder with a pulverizer. Mesh sieve silk.
[0030] (2) Weigh 350g of rhubarb, 250g of coptis, 150g of astragalus, 100g of ginger, and 100g of forsythia, add 2850ml of warm water at 60°C, stir well and put it in a water bath (60°C, 12 hours); or electric heating at 90°C Boil over low heat to make a paste, stir once a minute, add 100g of alum after cooling.
[0031] (3) Measure 1900ml of 95% alcohol, dissolve 10g of p-nitrophenol in it, then pour the alcoholic solution into the standby medicinal materials in step (2), stir well, pour into an airtight container and extract for 24 hours, and prepare Get the traditional Chinese medicine preservative for fish skinned specimens.
[0032] Instructions:
[0033] Taking the grass carp specimen as an example, peel off the skin of the grass ...
Embodiment 2
[0035] Weigh 50g each of rhubarb, coptis, astragalus, galangal, forsythia and alum respectively, place the above-mentioned traditional Chinese medicines in an oven at 60°C for 48 hours, crush them to below 40 mesh, put them into triangular flasks after weighing, and add 150ml of water was placed in a water bath (60°C, 12 hours), and then 250ml of 95% alcohol was added, sealed, extracted at 40°C, 100rpm constant temperature and vibration for 12 hours. Centrifuge at 3000r / min for 30 minutes, rotate the supernatant to 100% (W / V) concentration of the crude drug, and store in a sealed container at 4°C.
[0036] Preparation of nutrient broth medium, weigh 5g of beef extract, 5g of sodium chloride, 10g of peptone, and 10g of glucose, add water to 1000ml, boil to fully dissolve each component, and adjust the pH to 7.6-7.8 after cooling to room temperature. Then add agar 1.5-2%. For the cultivation of Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae. Potato medium...
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