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Method for preparing brown ginseng extract with high antitumor activity and application of method

A technology of extract and brown ginseng, which is applied in the field of medicine and health, can solve the problems of consumption of organic solvents, energy and resource waste of ginseng raw materials, complicated extraction and transformation technology and technological process, etc., and achieves high anti-tumor and combined anti-tumor activity. Effect

Inactive Publication Date: 2015-04-22
吉林玉参医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the industrialized production process proves that this product process method and production mode, extraction and transformation technology and process are complicated, consume organic solvents, energy and ginseng raw material resources are greatly wasted, and 1000 kg of ginseng can only extract 50 kg of total ginseng at most. Saponin, to be transformed into rare saponin Rg3 requires patented technology and further energy consumption

Method used

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  • Method for preparing brown ginseng extract with high antitumor activity and application of method
  • Method for preparing brown ginseng extract with high antitumor activity and application of method
  • Method for preparing brown ginseng extract with high antitumor activity and application of method

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Experimental program
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Effect test

Embodiment 1

[0018] Example 1 Preparation of brown ginseng extract

[0019] The common ginseng was pierced with needles, immersed in a container filled with glycosidase dissolved in phosphate buffer solution pH 8, and soaked for 4 h. Subsequently, the soaked ginseng was put into a device with pressurization capacity, and treated at 0.1 MPa, 121 °C for 4 h under high temperature and high pressure. The treated ginseng is dehydrated in a drying oven, dried and crushed in a pulverizer, then extracted with ethanol under ultrasonic conditions, and rotated under low pressure to obtain the brown ginseng extract;

[0020] Sample number: S1: Sun-dried ginseng without treatment;

[0021] S2: raw sun to participate in heat treatment;

[0022] S3: raw sun-dried ginseng + enzyme treatment + heating;

[0023] H1: No treatment for red ginseng;

[0024] H3: Red ginseng + enzyme treatment + heating.

Embodiment 2

[0025] Example 2 Cytotoxicity test

[0026] Tumor cell lines: A549, human non-small cell lung cancer cell line; MCF-7 / S, human breast cancer cell line sensitive to doxorubicin; K-562, human chronic myelogenous leukemia cell line;

[0027] Experimental method: in vitro anti-tumor cell experiment, adding ginseng extract samples at different doses;

[0028] Experimental results:

[0029]

[0030] The results of this experiment showed that for non-small cell lung cancer A549 cells, the anticancer activity of common ginseng was significantly improved (P<0.05) after heat treatment with enzymes. It shows that heating and enzyme treatment can improve the anti-A549 activity of common ginseng. For human leukemia K562 cells, the effects of treatment groups of raw sun-dried ginseng and red ginseng on the survival rate of K562 cells were significantly improved compared with the untreated group (P<0.05), indicating that raw sun-dried ginseng and red ginseng were treated with enzymes an...

Embodiment 3

[0031] Embodiment 3 animal model test

[0032] 1.1 Establishment of animal models

[0033] Tumor formation experiment of model mice H20 cells were purchased from Nanjing Kaiji Biotechnology Co., Ltd. 192 healthy mice (purchased from Dalian Medical University), all male, Kunming mice, weighing 18-22 g, were randomly divided into 8 groups, 12 in each group. Each mouse was subcutaneously inoculated with 0.2 ml (2 x l0 6 cells);

[0034] 1.2 Administration

[0035] 24 hours after inoculation, intragastric administration was started, 1 time / day, and the doses were Group A: normal saline 5 mL / kg body weight, Group B: brown ginseng extract 50 mg / kg; group C: brown ginseng extract 150 mg / kg , Group D: brown ginseng extract 300 mg / kg, group E: cyclophosphamide positive control (subcutaneous injection of cyclophosphamide 25mg / kg), group F: cyclophosphamide + brown ginseng extract 50mg / kg; group G: Cyclophosphamide + brown ginseng extract 150 mg / kg, group H: cyclophosphamide + bro...

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Abstract

The invention discloses a method for preparing a brown ginseng extract with high antitumor activity and an application of the method. The whole process is characterized in that the brown ginseng extract with high antitumor activity is quickly and conveniently prepared finally by the steps of enzymatic hydrolysis, high-temperature high-pressure heating, crushing, extraction and the like. Compared with untreated ginseng extracts, the activity of the extract obtained is increased by about 80 times. Cytotoxicity test and animal model experiment results show that compared with untreated common ginsengs, the brown ginseng extract has relatively high anti-tumor and combined anti-tumor activity, has an obvious inhibiting effect on formation of cyclophosphamide-induced bone marrow micronucleus of tumor-bearing mice and has a remarkable improving effect on immunosuppressive action induced by cyclophosphamide, which shows that the brown ginseng extract combined with cyclophosphamide in use can inhibit the toxic and side effects of cyclophosphamide and promote clinical application of cyclophosphamide.

Description

technical field [0001] The invention belongs to the technical field of medicine and health, in particular to the deep processing technology and technology of agricultural and sideline products. Background technique [0002] The type and content of ginsenoside (panaxoside) monomers are the main components that determine the efficacy of ginseng. A large number of studies have shown that ginsenoside Rg3, Rh2 and other important ginsenoside monomers have important anti-cancer and cardiovascular function regulation. Therefore, the isolation and chemical conversion of these monomers from ginseng and preparation of these monomers are important ginseng deep-processing products. However, the industrialized production process proves that this product process method and production mode, extraction and transformation technology and process are complicated, consume organic solvents, energy and ginseng raw material resources are greatly wasted, and 1000 kg of ginseng can only extract 5...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P35/00
CPCA61K36/258A61K2236/10A61K2236/15A61K2236/17A61K2236/19A61K2236/33
Inventor 霍虹窦同意丁乐乐
Owner 吉林玉参医药科技有限公司
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