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Preparation method of meso-porous silicon nanoparticle with reducing/enzyme dual response and targeting property

A nanoparticle and composite nanoparticle technology, applied in the field of preparation of mesoporous silicon nanoparticles, can solve the problems of large side effects, cell damage, etc., and achieve the effects of low cost, good stability, and long circulation time in the body

Inactive Publication Date: 2015-04-08
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the traditional smart mesoporous silicon drug-loaded particles show non-specific distribution in the body, which will cause great damage to normal tissues and cells, and have great side effects

Method used

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  • Preparation method of meso-porous silicon nanoparticle with reducing/enzyme dual response and targeting property

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Embodiment 1

[0029] Preparation of Magnetic Mesoporous Silicon Nanoparticles for Responsive Targeted Drug Delivery Capable of Magnetic Resonance Imaging. attached figure 1 TEM images of the prepared magnetic nanoparticles and magnetic mesoporous silicon composite particles.

[0030] (1) Fe(acac) 3 As a precursor, oleylamine is a reducing agent and a stabilizer, and diphenyl ether is a solvent, and oleylamine is used to prepare monodisperse ferric oxide nanoparticles stabilized by pyrolysis, wherein Fe(acac) 3 The molar ratio of oleylamine to oleylamine is 15:1, and diphenyl ether is 15mL;

[0031] (2) Fe stabilized by oleylamine 3 o 4 Conversion to CTAB-stabilized Fe 3 o 4 Nanoparticles, and add water, sodium hydroxide, tetraethyl orthosilicate in sequence, and grow mesoporous silicon shells on the surface by sol-gel method to obtain composite particles;

[0032] (3) Disperse the composite particles in absolute ethanol, add an appropriate amount of KH-550, and reflux at 80°C for 12...

Embodiment 2

[0038] Preparation of composite mesoporous silicon nanoparticles wrapped with gold nanorods for responsive targeted drug delivery with CT imaging function.

[0039] (1) Gold nanorods were prepared by seed growth method. The formula of the seed solution is as follows: 2.5 mL of CTAB solution with a concentration of 0.2 M, to which 1.5 mL of HAuCl with a concentration of 1.0 mM was added 4 solution, add 0.6 mL of ice-cold NaBH with a concentration of 0.01 M under vigorous stirring 4 solution, stirred for 2 minutes and then used after standing at 28°C for 2 hours; the growth solution formula is as follows: mix 50mL 0.2M CTAB, 2.8mL 4mM AgNO 3 , 5mL 15mM HAuCl 4 Mix with 45mL deionized water, add 1.25mL 0.08M ascorbic acid solution to it, mix well, add 1mL seed solution to it, and react at room temperature for 6h to obtain CTAB-stabilized gold nanorods;

[0040] (2) Using CTAB as a template, grow mesoporous silicon shells on gold rods. Take 15 mL of the prepared gold nanorods ...

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Abstract

The invention relates to a preparation method of meso-porous silicon nanoparticle with reducing / enzyme dual response and targeting property. The preparation method of the meso-porous silicon nanoparticle with the reducing / enzyme dual response and targeting property comprises the following steps: synthesizing a meso-porous silicon nanoparticle; modifying a disulfide bond on the surface of the meso-porous silicon nanoparticle by adopting a chemical method, and grafting the disulfide bond with hyaluronic acid as a targeting molecule; by taking carboxyl on the targeting molecule as a reaction site, modifying polyethylene glycol to the surface of the compound nanoparticle, thus improving biocompatibity of the nanoparticle, and connecting the hyaluronic acid molecule with the targeting effect to the surface of the nano particle, thus obtaining the meso-porous silicon nanoparticle diagnosis agent with medicinal response releasing and imaging functions. The prepared particle has good dispersity, is uniform in particle size and is easy to prepare, the prepared multifunctional diagnosis agent has good biocompatibility, medicine can be released in a tumour part in a responding manner, and diagnosis integration of the tumour can be realized.

Description

technical field [0001] The invention belongs to the field of preparation of nanometer materials with double response of reduction / enzyme, in particular to a preparation method of mesoporous silicon nanoparticles with double response of reduction / enzyme and targeting. Background technique [0002] Due to the advantages of large specific surface area, large pore volume, adjustable particle size, controllable shape, and good biocompatibility, mesoporous silicon nanoparticles have good application prospects in the field of drug delivery. In order to achieve "zero release" before the drug reaches the target site, people design various "gates" on the surface of mesoporous silicon, and construct a stimuli-responsive smart drug controlled release system. Since Tanaka et al. (Nature 2003, 421, 350–353) used coumarin as the “gateway” of mesoporous silicon to construct a light-responsive mesoporous silicon controlled release system in 2003, various stimuli-responsive mesoporous silicon...

Claims

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Application Information

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IPC IPC(8): A61K49/08A61K49/18A61K49/00A61K9/51A61K47/04A61K47/36A61K31/4745A61K49/04A61P35/00
Inventor 何创龙陈良冯炜贾亚听常安妮
Owner DONGHUA UNIV
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