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Online sweeping-micellar electrokinetic chromatography method for separating and measuring protocatechuic acid in acute bronchitis syrup

A protocatechuic acid and electrokinetic chromatography technology, which is applied in the direction of material separation, material analysis by electromagnetic means, and measuring devices, can solve the problems of reduced detection sensitivity, small inner diameter of capillary, short detection optical path, etc., and achieves high sensitivity, Reproducible and easy-to-use results

Inactive Publication Date: 2015-03-25
GUANGXI UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

However, CE also has some disadvantages. Its small capillary inner diameter makes the volume of sample injection relatively small, and also makes its detection optical path relatively short, thereby reducing the detection sensitivity of CE.

Method used

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Experimental program
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Embodiment 1

[0008] On-line sweeping-micelle capillary electrokinetic chromatography for separation and determination of protocatechuic acid in Jizhi syrup. In the micellar capillary electrophoresis, the electrophoresis conditions are: the buffer system is 20 mmol / L sodium dihydrogen phosphate at pH=2.2 + 80 mmol / L SDS + 12.5% ​​acetonitrile (V / V); the detection wavelength is 225nm; the ambient temperature is 25 °C; online sweeping-micelle capillary chromatography is to dilute the standard and sample solutions to be measured with water to a suitable concentration, and the separation voltage is - 20 kV, gravity injection, injection height 16 cm, injection time 60 s; the detection limit of protocatechuic acid was 15.96 μg / L.

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Abstract

The invention discloses an online sweeping-micellar electrokinetic chromatography method for separating and measuring protocatechuic acid in acute bronchitis syrup. In micelle capillary electrophoresis, the electrophoresis conditions are that the buffer system refers to 20mmol / L sodium dihydrogen phosphate, 80mmol / L SDS and 12.5 percent (V / V) of acetonitrile with the pH value of 2.2; the detection wavelength is 225nm; the ambient temperature is 25 DEG C. The online sweeping-micellar electrokinetic chromatography method comprises the following steps: diluting a to-be-detected standard substance and sample solution to a proper concentration by using water, wherein the separation voltage is -20kV; and performing gravity injection, wherein the injection height is 16cm, and the injection time is 60 seconds; and the detection limit of protocatechuic acid is 15.96mu g / L. The method disclosed by the invention has the beneficial effects that the online sweeping-micellar electrokinetic chromatography method has good enrichment effect and detection limit, and the method is simple, rapid, high in sensitivity and high in reproducibility and can be used for separating protocatechuic acid and measuring content of protocatechuic acid in cough medicines.

Description

technical field [0001] The invention relates to the separation and determination of protocatechuic acid in Jizhi syrup by on-line sweeping-micelle capillary electrokinetic chromatography. Background technique [0002] Jizhi syrup is a compound preparation made from Houttuynia cordata, golden buckwheat, Sijiqing, ephedra, peucedanum, licorice and other medicinal materials. The active ingredients are ferulic acid, protocatechualdehyde, protocatechuic acid and so on. Ferulic acid, protocatechualdehyde and protocatechuic acid all have various pharmacological effects. Pharmacological experimental studies have shown that ferulic acid, protocatechuic aldehyde and protocatechuic acid are water-soluble substances, which have pharmacological activities such as anti-inflammatory, anti-rheumatic, promoting platelet depolymerization, and enhancing human sperm vitality and motility. The research of the three substances is quite extensive. [0003] In recent years, the main methods for ...

Claims

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Application Information

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IPC IPC(8): G01N30/00G01N27/447
Inventor 李利军张瑞瑞
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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