Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Freeze-dried powder for treating focal cerebral ischemia reperfusion inflammation reaction and preparation method thereof

A technology for focal cerebral ischemia and inflammatory response, applied in the field of medicine, can solve the problems of restricting the popularization and application of thrombolytic therapy and short treatment time, and achieve the effects of reducing cerebral infarction volume, reducing inflammatory response and good therapeutic effect

Inactive Publication Date: 2015-03-11
JINING MEDICAL UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, ultra-early intravenous administration of recombinant tissue-type plasminogen activator thrombolytic therapy is the only drug therapy supported by evidence-based medicine and approved by the US Food and Drug Administration for the treatment of acute ischemic stroke, but The short treatment time is the biggest bottleneck restricting thrombolytic therapy, and the secondary nerve injury caused by reperfusion after thrombolysis also limits the popularization and application of thrombolytic therapy.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Freeze-dried powder for treating focal cerebral ischemia reperfusion inflammation reaction and preparation method thereof
  • Freeze-dried powder for treating focal cerebral ischemia reperfusion inflammation reaction and preparation method thereof
  • Freeze-dried powder for treating focal cerebral ischemia reperfusion inflammation reaction and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036]The present invention also includes a preparation method of freeze-dried powder for treating focal cerebral ischemia-reperfusion inflammatory reaction, comprising the following steps:

[0037] (1) Add 1-5 parts by weight of Apelin-13, 5-20 parts of human serum albumin, 5-10 parts of mannitol, 1-5 parts of dextran and 5-15 parts of arginine into 300-500 parts dissolved in water for injection to obtain a mixed liquid medicine;

[0038] (2) Add 0.02 to 0.05% activated carbon for injection to the mixed medicinal liquid obtained in step (1), stir and absorb, and filter to obtain the filtrate;

[0039] (3) Store the filtrate in step (2) under sterile conditions with a microporous membrane filter at 0 to 5° C. to obtain a microfiltered solution;

[0040] (4) Divide the microfiltered solution and place it on the plate of the product chamber of the freeze dryer, lower the temperature of the condenser to -30°C at a rate of 15°C per hour, keep it warm for 1 to 2 hours, and then re...

Embodiment 1

[0061] A freeze-dried powder for treating focal cerebral ischemia-reperfusion inflammatory reaction, which consists of the following components: 1 part of Apelin-13, 20 parts of human serum albumin, 5 parts of mannitol, 5 parts of dextran and 15 parts of arginine .

[0062] The preparation steps are as follows:

[0063] (1) 1 part by weight of Apelin-13, 20 parts of human serum albumin, 5 parts of mannitol, 5 parts of dextran and 15 parts of arginine were dissolved in 300 parts of water for injection to obtain a mixed medicinal solution;

[0064] (2) Add 0.05% activated carbon for injection to the mixed medicinal liquid obtained in step (1), stir and absorb, and filter to obtain the filtrate;

[0065] (3) Store the filtrate in step (2) under sterile conditions with a microporous membrane filter at 0 to 5° C. to obtain a microfiltered solution;

[0066] (4) Divide the microfiltered solution and place it on the product chamber plate of the freeze dryer, lower the temperature o...

Embodiment 2

[0068] A freeze-dried powder for treating focal cerebral ischemia-reperfusion inflammation, which consists of the following components in parts by weight: 5 parts of Apelin-13, 5 parts of human serum albumin, 10 parts of mannitol, 1 part of dextran and Arginine 5 servings.

[0069] The preparation steps are as follows:

[0070] (1) 5 parts by weight of Apelin-13, 5 parts of human serum albumin, 10 parts of mannitol, 1 part of dextran and 5 parts of arginine are dissolved in 500 parts of water for injection to obtain a mixed medicinal solution;

[0071] (2) Add 0.02% activated carbon for injection to the mixed medicinal liquid obtained in step (1), stir and adsorb, filter to obtain the filtrate;

[0072] (3) Store the filtrate in step (2) under sterile conditions with a microporous membrane filter at 0 to 5° C. to obtain a microfiltered solution;

[0073] (4) Divide the microfiltered solution and place it on the plate of the product chamber of the freeze dryer, lower the temp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a freeze-dried powder for treating focal cerebral ischemia reperfusion inflammation reaction and a preparation method thereof. The freeze-dried powder is composed of Apelin-13, human serum albumin, mannitol, dextran and arginine. The freeze-dried powder is prepared by steps of raw materials mixing, activated carbon adsorption, filtering, micro-filtration and freeze drying. The freeze-dried powder can obviously reduce cerebral infarction volume and has good treatment effect for treating focal cerebral ischemia reperfusion inflammation injury; the freeze-dried powder can reduce MPO activity in hippocampus, performs up-regulation of expression level of APJ protein, decreases Iba1, GFAP and HMGB1 protein expression level; inhibits activation, adhesion and aggregation of neutral granulocyte in a cerebral ischemia zone, minimizes release of cytotoxic substances, mitigates inflammation reaction in an ischemic zone and performs neuroprotection effect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a freeze-dried powder for treating focal cerebral ischemia-reperfusion inflammation and a preparation method thereof. Background technique [0002] Ischemic stroke is currently one of the important causes of human disability and death. Most acute cerebral infarctions are caused by thrombus or embolus blocking cerebral arteries. Cerebral blood vessels, timely restoration of cerebral blood flow, is expected to avoid necrosis of ischemic brain tissue. So far, ultra-early intravenous administration of recombinant tissue-type plasminogen activator thrombolytic therapy is the only drug therapy supported by evidence-based medicine and approved by the US Food and Drug Administration for the treatment of acute ischemic stroke, but The short treatment time is the biggest bottleneck restricting thrombolytic therapy, and the secondary nerve injury caused by reperfusion after thrombolysis a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/38A61K38/10A61K9/19A61P9/10
Inventor 白波陈京刘文彦程葆华王春梅路海武菲潘衍有杨春青薛建军薛庆节
Owner JINING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com