Solid-state fermentation method for feeding bacillus subtilis with high germination rate and high stress resistance
A Bacillus subtilis, solid-state fermentation technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve the problems that plague the industrial production of Bacillus subtilis, the production effect is not ideal, etc., and solve the problem of bacterial growth. Metabolic stagnation, high survival rate, effect of improving spore yield
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Embodiment 1
[0028] (1) Bacterial strain: Bacillus subtilis CICC 10453 was selected and purchased from China Industrial Microorganism Culture Collection Management Center;
[0029] (2) Streak the bacterial strain described in step (1) in three zones on the nutrient agar medium, and culture at 37° C. for 24 hours;
[0030] Under sterile conditions, pick a single colony and inoculate it into 20 mL of nutrient broth medium, shake it at 37°C and 180 rpm for 5 hours to obtain the primary seed liquid;
[0031] Inoculate the primary seed liquid in the nutrient broth medium with 5% inoculum amount, shake and culture at 37°C and 180rpm for 4 hours to obtain the secondary seed liquid;
[0032] The above-mentioned nutrient agar medium is composed of 0.3% beef extract, 1.0% peptone, 0.5% sodium chloride, 2.0% agar, prepared in distilled water, pH 7.2-7.4; the nutrient broth medium is composed of 0.3% beef extract, 1.0 peptone %, sodium chloride 0.5%, prepared with distilled water, pH 7.2-7.4;
[003...
Embodiment 2
[0040] (1) Bacterial strain: Bacillus subtilis CICC 10453 was selected and purchased from China Industrial Microorganism Culture Collection Management Center;
[0041] (2) Streak the bacterial strain described in step (1) in three zones on the nutrient agar medium, and culture at 37° C. for 24 hours;
[0042] Under sterile conditions, pick a single colony and inoculate it into 20 mL of nutrient broth medium, shake it at 37°C and 180 rpm for 6 hours to obtain the primary seed liquid;
[0043]Inoculate the primary seed liquid in the nutrient broth medium with 1% inoculation amount, shake and culture at 37°C and 180rpm for 6 hours to obtain the secondary seed liquid;
[0044] The above-mentioned nutrient agar medium is composed of 0.3% beef extract, 1.0% peptone, 0.5% sodium chloride, 2.0% agar, prepared in distilled water, pH 7.2-7.4; the nutrient broth medium is composed of 0.3% beef extract, 1.0 peptone %, sodium chloride 0.5%, prepared with distilled water, pH 7.2-7.4;
[0...
Embodiment 3
[0050] (1) Bacterial strain: Bacillus subtilis CICC 10453 was selected and purchased from China Industrial Microorganism Culture Collection Management Center;
[0051] (2) Streak the bacterial strain described in step (1) in three zones on the nutrient agar medium, and culture at 37° C. for 24 hours;
[0052] Under sterile conditions, pick a single colony and inoculate it into 20 mL of nutrient broth medium, shake it at 37°C and 160 rpm for 8 hours to obtain a first-grade seed liquid;
[0053] Inoculate the primary seed liquid in the nutrient broth medium with 2% inoculation amount, shake and culture at 37°C and 160 rpm for 4 hours to obtain the secondary seed liquid;
[0054] The above-mentioned nutrient agar medium is composed of 0.3% beef extract, 1.0% peptone, 0.5% sodium chloride, 2.0% agar, prepared in distilled water, pH 7.2-7.4; the nutrient broth medium is composed of 0.3% beef extract, 1.0 peptone %, sodium chloride 0.5%, prepared with distilled water, pH 7.2-7.4; ...
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