Biological fermentation protein feed and preparation method thereof
A biological fermentation protein and feed technology, applied in the field of biotechnology fermentation engineering, can solve the problems of lower bioavailability, fishy smell of blood, poor palatability, etc., and achieve the effect of improving feeding value, promoting feed intake, and low cost
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Embodiment 1
[0030] A preparation method of biologically fermented protein feed, comprising the following steps:
[0031] (1) Cultivation of four microorganisms
[0032] 1L solid medium contains the following ingredients: glucose 20g, peptone 10g, yeast extract 5g, beef extract 10g, anhydrous sodium acetate 5g, diammonium citrate 2g, Tween 801mL, MgSO 4 ·7H 2 O 0.58g, K 2 HPO 4 2g, 20g of agar powder; the pH value of the medium is 6.2-6.4.
[0033] 1L liquid medium includes the following ingredients: glucose 20g, peptone 10g, yeast extract 5g, beef extract 10g, anhydrous sodium acetate 5g, diammonium citrate 2g, Tween 801mL, MgSO 4 ·7H 2 O 0.58g, K 2 HPO 4 2g; the pH value of the medium is 6.2-6.4.
[0034] The prepared culture was sterilized in an autoclave at 121°C for 15 minutes.
[0035] Activation of strains: After sterilizing the solid medium under ultraviolet light for 20 minutes, pour it onto the plate. After the medium in the plate cools down, inoculate yeast, Bacillus su...
Embodiment 2
[0049] A preparation method of biologically fermented protein feed, comprising the following steps:
[0050] (1) Cultivation of four microorganisms
[0051]1L solid medium contains the following ingredients: glucose 20g, peptone 10g, yeast extract 5g, beef extract 10g, anhydrous sodium acetate 5g, diammonium citrate 2g, Tween 801mL, MgSO 4 ·7H 2 O 0.58g, K 2 HPO 4 2g, 20g of agar powder; the pH value of the medium is 6.2-6.4.
[0052] 1L liquid medium includes the following ingredients: glucose 20g, peptone 10g, yeast extract 5g, beef extract 10g, anhydrous sodium acetate 5g, diammonium citrate 2g, Tween 801mL, MgSO 4 ·7H 2 O 0.58g, K 2 HPO 4 2g; the pH value of the medium is 6.2-6.4.
[0053] The prepared culture was sterilized in an autoclave at 121°C for 15 minutes.
[0054] Activation of strains: After sterilizing the solid medium under ultraviolet light for 20 minutes, pour it onto the plate. After the medium in the plate cools down, inoculate yeast, Bacillus sub...
Embodiment 3
[0068] A preparation method of biologically fermented protein feed, comprising the following steps:
[0069] (1) Cultivation of four microorganisms
[0070] 1L solid medium contains the following ingredients: glucose 20g, peptone 10g, yeast extract 5g, beef extract 10g, anhydrous sodium acetate 5g, diammonium citrate 2g, Tween 801mL, MgSO 4 ·7H 2 O 0.58g, K 2 HPO 4 2g, 20g of agar powder; the pH value of the medium is 6.2-6.4.
[0071] 1L liquid medium includes the following ingredients: glucose 20g, peptone 10g, yeast extract 5g, beef extract 10g, anhydrous sodium acetate 5g, diammonium citrate 2g, Tween 801mL, MgSO 4 ·7H 2 O 0.58g, K 2 HPO 4 2g; the pH value of the medium is 6.2-6.4.
[0072] The prepared culture was sterilized in an autoclave at 121°C for 15 minutes.
[0073] Activation of strains: After sterilizing the solid medium under ultraviolet light for 20 minutes, pour it onto the plate. After the medium in the plate cools down, inoculate yeast, Bacillus su...
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