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An injectable hydrogel with controlled release capability for tissue engineering and its application

A tissue engineering and injection technology, applied in the field of biomedical engineering materials, can solve problems such as poor mechanical properties of collagen gels, achieve good biocompatibility and degradability, excellent controllability, gel time controllable effect

Inactive Publication Date: 2016-08-10
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical properties of collagen-based gels are poor. How to improve the mechanical properties of collagen-based gels so that they can better support the new soft tissue structure has not yet been well resolved.

Method used

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  • An injectable hydrogel with controlled release capability for tissue engineering and its application
  • An injectable hydrogel with controlled release capability for tissue engineering and its application
  • An injectable hydrogel with controlled release capability for tissue engineering and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The synthesis of embodiment 1 gelatin-tyramine-heparin complex

[0051] The concrete steps of synthetic method are as follows:

[0052] (1) Synthesis of gelatin-tyramide complex

[0053] Weigh 4g of B-type gelatin and dissolve it in 50mM 2-(N-morpholine)ethanesulfonic acid (MES) solution, heat to 60°C to fully dissolve it, then cool to room temperature, weigh 1g of tyramine and dissolve it in the above solution, and Add cross-linking agent (EDC / NHS) and stir at room temperature for 12-24 hours; put the reacted solution into a dialysis bag with a molecular weight cut-off of 10,000 and dialyze in deionized water for 2-3 days, freeze-dry the sample after the dialysis, Obtain gelatin-tyramide complex;

[0054] (2) Synthesis of gelatin-tyramine-heparin complex

[0055] Weigh 4 g of the gelatin-tyramine complex prepared in step (1) and dissolve it in 50 mM 2-(N-morpholine) ethanesulfonic acid (MES) solution, heat to 60° C. to fully dissolve it, then cool to room temperatur...

Embodiment 2

[0057] Example 2 Preparation of Injectable Hydrogel with Controlled Release Capability for Tissue Engineering

[0058] Dissolve the gelatin-tyramine-heparin complex synthesized in Example 1 in deionized water to form a gel solution with a mass fraction of 2%, add 1 unit / ml of HRP, mix well, and then add 1mM H 2 o 2 , forming a hydrogel within five minutes, the chemical reaction formula of the preparation method is as follows figure 2 As shown, the hydrogel can be injected using a syringe.

Embodiment 3

[0059] Example 3 Effect of polymer (gelatin-tyramine-heparin complex) concentration on hydrogel pores

[0060] Dissolve the gelatin-tyramine-heparin complexes synthesized in Example 1 in deionized water to form gel solutions with mass fractions of 2.5%, 5%, 7.5%, and 10%, respectively, and then add 1 unit / ml HRP, mix well, then add 1mM H 2 o 2 , the hydrogel can be formed, and the hydrogel pores formed by gel solutions with different mass fractions are as follows image 3 shown. The mechanical strength of hydrogels can be tuned by changing the degree of crosslinking, such as Figure 4 The appearance of hydrogels with different mechanical strengths is given in .

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PUM

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Abstract

The invention belongs to the field of biomedical engineering materials, and specifically relates to an injectable hydrogel with controlled release capacity and for tissue engineering, and an application thereof. The injectable hydrogel (shown as a formula I) with controlled release capacity and for the tissue engineering is formed by bonding two or more polymers shown as the formula II via dehydrogenation reactions between phenol or aniline parts of adjacent polymers. The polymers shown as the formula II are formed by co-grafting heparin and tyramine on skeleton of the polymers in a parallel manner. The injectable hydrogel with controlled release capacity and for the tissue engineering can form a gel in situ after being injected, has good controlled release capacity and good biological stability and mechanical strength. The formula I and the formula II are shown in the description.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, and in particular relates to an injectable hydrogel with controlled release capability for tissue engineering tools and an application thereof. Background technique [0002] Soft tissue defects caused by various reasons such as trauma, tumor resection, congenital malformation, and aging not only affect the appearance of the patient, but also affect the mental health of the patient, and even affect the function of the body in severe cases. [0003] In recent years, the use of tissue engineering principles and injectable degradable biomaterials has provided a new idea for the repair of soft tissue defects. Injectable biomaterials can form hydrogel-like substances through physical or chemical cross-linking methods after injection into the body, and these materials have good biocompatibility, biodegradability and can provide good seed cells. Space living environment. Due to their hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/22A61L27/20A61L27/52A61L27/54A61L31/04A61L31/14A61L31/16A61K47/36A61K9/06C08J3/075C08J3/24C08G81/00C08H1/00
Inventor 李喆刘晓华李世荣毋巨龙王珍祥
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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