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Colloidal gold immunochromatography quick test card for pyrethriods pesticides, and preparation method and using method of quick test card

A technology of pyrethroids and immunochromatography, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of low sensitivity, inconvenient operation, and poor specificity, and achieve high sensitivity, improved specificity, and stability Improved effect

Active Publication Date: 2015-01-21
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are still many problems in applying the existing immune colloidal gold technology to the detection of pesticide residues, such as poor specificity, poor stability, low sensitivity, high cost, and inconvenient operation. Therefore, those skilled in the art urgently need to understand the existing Immunogold technology was improved to develop a new method for the detection of various pyrethroid pesticides with strong specificity, high sensitivity, good stability and good repeatability

Method used

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  • Colloidal gold immunochromatography quick test card for pyrethriods pesticides, and preparation method and using method of quick test card
  • Colloidal gold immunochromatography quick test card for pyrethriods pesticides, and preparation method and using method of quick test card
  • Colloidal gold immunochromatography quick test card for pyrethriods pesticides, and preparation method and using method of quick test card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] see Figure 1-7 , a pyrethroid pesticide colloidal gold immunochromatography quick test card, which includes a stuck case 1 and a test paper strip 2 inside the stuck case 1, wherein the test paper 2 includes a bottom plate 3, and samples adhered to the bottom plate 3 in sequence Pad 4, gold standard pad 5, reaction membrane 6 and absorbent pad 7. The cartridge 1 is provided with a sample addition hole 8 and an observation window 9, the sample addition hole 8 is arranged on the upper casing corresponding to the sample pad 4, and the observation window 9 is arranged on the upper casing corresponding to the reaction membrane 6 . A repeating area of ​​1-2 mm is provided between the sequentially connected sample pad 4 , gold standard pad 5 , reaction membrane 6 and water-absorbing pad 7 . The bottom plate 3 is a PVC plate, and the reaction membrane 6 is a nitrocellulose membrane. The sample pad 4 is made of glass fiber or filter paper, and the absorbent pad 7 is made of f...

Embodiment 2

[0054] Example 2 Sensitivity Test of Colloidal Gold Immunochromatography Quick Test Card for Pyrethroid Pesticides

[0055] Prepare pyrethroid pesticide colloidal gold immunochromatography quick test card according to the method in Example 1. The colloidal gold immunochromatography quick test card was tested separately for cypermethrin, fenvalerate, deltamethrin, phenemethrin, cyfluthrin, cyfluthrin, cyfluthrin, fenvalerate, fluramine Detection sensitivity of 9 kinds of pyrethroid pesticides including cyanothrin. The nine kinds of pyrethroid pesticide standards were diluted to a certain concentration, and the lowest concentration that could detect a positive result was the detection sensitivity of the quick test card for the pesticide.

[0056] After testing, the test results of 9 permethrin pesticides were all positive, and the detection limits are shown in Table 1: For 10mg / kg cypermethrin, fenpropathrin, deltamethrin, phenemethrin, cyfluthrin, and cyfluthrin , fenvalerate...

Embodiment 3

[0059] Example 3: Specificity test of colloidal gold immunochromatography quick test card for pyrethroid pesticides

[0060] Prepare pyrethroid pesticide colloidal gold immunochromatography quick test card according to the method in Example 1. DDT, HCH and other organochlorine pesticides and organophosphorus pesticides such as chlorpyrifos, methamidophos, dimethoate, dichlorvos, and malathion, and carbamic acids such as carbaryl, carbofuran, and methomyl The ester pesticides were diluted to a certain concentration and tested with a quick test card.

[0061] The test results show that the positive detection rate of the quick test card for the samples of organochlorine, organophosphorus and carbamate pesticides with a concentration of 1 mg / ml is all lower than 0.5%, that is, the cross-reaction rate is lower than 0.5%. On the surface, the quick test card has strong detection specificity for pyrethroid pesticides, and is suitable for specific residue detection of various pyrethro...

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Abstract

The invention relates to a colloidal gold immunochromatography quick test card for pyrethriods pesticides, and a preparation method and a using method of the quick test card. The quick test card comprises a card shell and a test strip in the card shell, wherein the test strip comprises a bottom plate, and a sample pad, a gold-labeled pad, a reaction film and a water absorption pad which are stuck onto the bottom plate and sequentially connected with one another; the gold-labeled pad is enveloped by a pyrethriods pesticide gold-labeled wide-spectrum antibody; a detection line of the reaction film is enveloped by a pyrethriods pesticide antibody; a control line of the reaction film is enveloped by a goat anti-rabbit second antibody; the pyrethriods pesticide gold-labeled wide-spectrum antibody is enveloped by nanoscale chloroauric acid colloidal particles. After the colloidal gold immunochromatography quick test card is used, at least nine pyrethriods pesticides can be detected at the same time, and the limit of detection reaches up to 0.01mg / kg; the colloidal gold immunochromatography quick test card can be stably stored for more than 1.5 years at room temperature, and is high in specificity and sensitivity, good in stability, simple, convenient and accurate in operation and short in detection time, thus being widely used for rapidly detecting pyrethriods residues in agricultural products, water environment and soil.

Description

technical field [0001] The invention belongs to the technical field of detection of pyrethroid pesticide residues, and in particular relates to a colloidal gold immunochromatography quick test card for pyrethroid pesticides, a preparation method and a use method thereof. Background technique [0002] Pyrethroid pesticides (Pyrethriod insecticids) are a class of natural biomimetic insecticides, which have the characteristics of fast insecticide, low toxicity, and safe use. It is one of the types of pesticides with a high detection rate of pesticide residues in agricultural products in my country. At present, the output of pyrethroid pesticides accounts for 20% of the output of insecticides, and the application area accounts for about 25% of the total area of ​​pesticide application. Since some pyrethroid pesticides have a long residual period, are highly toxic to non-target organisms, and have environmental endocrine disrupting effects, it is of great significance to establi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/558
Inventor 唐雪明王金斌武国干刘华吴潇赵凯李鹏吕贝贝蒋玮
Owner SHANGHAI ACAD OF AGRI SCI
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