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Novel Dictyophora rubrovolvata polysaccharide with immunocompetence, and preparation method and application thereof

A kind of technology of bamboo fungus and immune activity, which is applied in the field of immune activity polysaccharide and its preparation, and achieves the effect of simple and reasonable preparation process, high purity and good immune promotion effect

Active Publication Date: 2015-01-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] To sum up, at present, the fruiting body of Dictyophora edulis is used as raw material, followed by hot water extraction, Savege method to remove protein, ethanol precipitation, and then "ion exchange column chromatography-gel filtration column chromatography-ultrafiltration Separation-Gel Filtration Column Chromatography" four-step separation method to obtain after separation and purification, and there is no report on the immune activity experiment of mouse macrophages with the separated and purified Dictyophora polysaccharide

Method used

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  • Novel Dictyophora rubrovolvata polysaccharide with immunocompetence, and preparation method and application thereof
  • Novel Dictyophora rubrovolvata polysaccharide with immunocompetence, and preparation method and application thereof
  • Novel Dictyophora rubrovolvata polysaccharide with immunocompetence, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0055] (1) Dried 100 g of Dictyophora edulis at 40 °C for 2 h, and then pulverized it with a powder machine;

[0056] (2) Extract in hot water at 100 °C for 1 h, the ratio of solid to liquid is 1 : 40 g / mL, and the number of extractions is 1 time; centrifuge at 4000 r / min for 15 min, collect the supernatant and depressurize by rotary evaporation at 60 °C concentrate.

[0057] (3) Use the Sevage method to deproteinize the hot water extract. The preparation method of the Sevage reagent is to mix chloroform and n-butanol in a volume ratio of 4:1; Mix 1:1, shake for 20 min, centrifuge at 3000 r / min for 10 min, take out the polysaccharide solution in the upper layer, and dry it to obtain the crude polysaccharide of Dictyophora; the deproteinization process is repeated 10 times.

[0058] (4) Add 3 times the volume of absolute ethanol to the crude polysaccharide solution after deproteinization, and let stand overnight at 4 °C; then centrifuge at 4000 r / min for 20 min, collect the pr...

Embodiment 2

[0065] (1) Dry 100 g of Dictyophora hongtuo fruiting body at 70 ℃ for 0.5 h, and then pulverize it with a powder machine;

[0066] (2) Extract in hot water at 90 °C for 2 h, the ratio of solid to liquid is 1 : 20 g / mL, and the number of extractions is 3 times; centrifuge at 4000 r / min for 15 min, collect the supernatant and depressurize by rotary evaporation at 60 °C concentrate.

[0067] (3) Use the Sevage method to deproteinize the hot water extract. The preparation method of the Sevage reagent is to mix chloroform and n-butanol in a volume ratio of 4:1; Mix 1:1, shake for 20 min, centrifuge at 3000 r / min for 10 min, take out the polysaccharide solution in the upper layer, and dry it to obtain the crude polysaccharide of Dictyophora; the deproteinization process is repeated 10 times.

[0068] (4) Add 3 times the volume of absolute ethanol to the crude polysaccharide solution after deproteinization, and let stand overnight at 4 °C; then centrifuge at 8000 r / min for 10 min, c...

Embodiment 3

[0074] (1) Dry 100 g of the fruiting body of Dictyophora edulis at 60 °C for 3 h, and then pulverize it with a powder machine;

[0075] (2) Extract in hot water at 100 °C for 4 h, the ratio of solid to liquid is 1 : 30 g / mL, and the number of extraction times is 3 times; centrifuge at 8000 r / min for 10 min, collect the supernatant and depressurize by rotary evaporation at 60 °C concentrate.

[0076] (3) Use the Sevage method to deproteinize the hot water extract. The preparation method of the Sevage reagent is to mix chloroform and n-butanol in a volume ratio of 4:1; Mix 1:1, shake for 20 min, centrifuge at 3000 r / min for 10 min, take out the polysaccharide solution in the upper layer, and dry it to obtain the crude polysaccharide of Dictyophora; the deproteinization process is repeated 10 times.

[0077] (4) Add 3 times the volume of absolute ethanol to the crude polysaccharide solution after deproteinization, and let stand overnight at 4 °C; then centrifuge at 4000 r / min fo...

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Abstract

The invention discloses a novel Dictyophora rubrovolvata polysaccharide with immunocompetence, and a preparation method and application thereof. The method uses Dictyophora rubrovolvata as the raw material and comprises the following steps: leaching with hot water, removing proteins by a Savege process, carrying out ethanol precipitation, and carrying out separation and purification by ion-exchange column chromatography, gel filtration chromatography, ultrafiltration separation and gel filtration chromatography. The preparation technique is reasonable and simple, and can easily implement industrialized mass production. The novel Dictyophora rubrovolvata polysaccharide has favorable immunological enhancement action, and can be used as an auxiliary therapeutic drug for tumors and other immunocompromised diseases or as a functional health food or food capable of enhancing immunity.

Description

technical field [0001] The invention relates to an immunologically active polysaccharide and its preparation method and application, in particular to a new immunoactive Dictyophora polysaccharide as well as its preparation method and application. Background technique [0002] Dictyophora( Dictyophora indusiata ), alias "bamboo fungus", is a general term for a large class of fungi of the genus Dictyophora, belonging to the fungal phylum ( Eumycota ), Basidiomycotina ( Basidiomycotin a), Abdomycetes ( Gasteromycetes ), Ghostwriting ( Phallales ), Phallus Branch ( Phallaceae ). [0003] Dictyophora is a cryptogamous fungus parasitic on the roots of dry bamboos. Its shape is slightly resembling a net-shaped dry white snakeskin. It has a dark green cap, a snow-white cylindrical stipe, and a pink egg-shaped receptacle. There is a circumference at the top of the stipe [0004] The fine and white mesh skirt spreads downward from the mushroom cap, and is called "Snow Skir...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P35/00A23L1/09A23L29/30A23L33/125
Inventor 杨继国林泽华廖文镇任娇艳张榕宁正祥
Owner SOUTH CHINA UNIV OF TECH
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