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Fermented Radix Astragali preparation method, and method for extracting total saponins of fermented Radix Astragali

An extraction method and technology for astragalus, which are applied in the field of preparation of fermented astragalus, can solve problems such as no research report, unclear changes of total saponins, etc., and achieve the effects of high extraction efficiency, favorable utilization and high purity

Inactive Publication Date: 2014-11-19
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]A lot of enzymes secreted by non-lactolytic streptococcus LZMYFGM9 (preservation number: CGMCC No.4227) can participate in the biotransformation process. The change of the total saponins is not clear at present, and there is no research report on the separation and extraction method of the saponins of Astragalus fermented by this bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation method of fermented Radix Astragali of the present invention is:

[0051] After crushing the clean astragalus crude drug pieces, passing through a 20-mesh sieve, fermenting astragalus in a 10L fermenter, the concentration of the fermented strain LZMYFGM9 is 2×10 8 each / mL, the inoculation amount is 5% (accounting for the total fermentation volume), the dosage of Astragalus is 8% (the mass of Astragalus: the volume of fermentation broth, g:mL), the temperature is 39°C, the rotation speed is 200r / min, the pH value is 6.4, and the dissolved oxygen The concentration is 10%, the neutralizing agent is 6mol / L NaOH, and the fermentation time is 48h. After the fermentation is finished, the fermented astragalus liquid is freeze-dried to obtain the freeze-dried fermented astragalus powder. The mass fraction of astragalus in fermented astragalus was measured to be 78%, and the mass of astragalus contained in 400 g of fermented astragalus freeze-dried powder...

Embodiment 2

[0055] The preparation method of fermented Radix Astragali of the present invention is:

[0056] After crushing the clean astragalus crude drug pieces, passing through a 20-mesh sieve, fermenting astragalus in a 10L fermenter, the concentration of the fermented strain LZMYFGM9 is 2×10 6 each / mL, the inoculation amount is 4% (accounting for the total fermentation volume), the dosage of Astragalus is 8% (the mass of Astragalus: the volume of fermentation broth, g:mL), the temperature is 38°C, the rotation speed is 200r / min, the pH value is 5.5, and the dissolved oxygen 5%, the neutralizing agent is 6mol / L NaOH, and the fermentation time is 44h. After the fermentation is finished, the fermented astragalus liquid is freeze-dried to obtain the freeze-dried fermented astragalus powder. The mass fraction of astragalus in fermented astragalus was measured to be 78%, and the mass of astragalus contained in 400 g of fermented astragalus freeze-dried powder was 312 g.

[0057] ...

Embodiment 3

[0060] The preparation method of fermented Radix Astragali of the present invention is:

[0061] After crushing the clean astragalus crude drug pieces, passing through a 20-mesh sieve, fermenting astragalus in a 10L fermenter, the concentration of the fermented strain LZMYFGM9 is 2×10 7 each / mL, the inoculation amount is 4.5% (accounting for the total fermentation volume), the dosage of Astragalus is 8% (the mass of Astragalus: the volume of fermentation broth, g:mL), the temperature is 39°C, the rotation speed is 200r / min, the pH value is 6.0, and the dissolved oxygen The concentration is 8%, the neutralizing agent is 6mol / L NaOH, and the fermentation time is 52h. After the fermentation is finished, the fermented astragalus liquid is freeze-dried to obtain the freeze-dried fermented astragalus powder. The mass fraction of astragalus in fermented astragalus was measured to be 78%, and the mass of astragalus contained in 400 g of fermented astragalus freeze-dried powde...

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Abstract

The invention provides a fermented Radix Astragali preparation method. Fermented Radix Astragali is obtained through fermenting Radix Astragali by Streptococcus alactolyticus strains LZMYFGM9 with the preservation number of CGMCC No.4227. The invention also provides a method for extracting total saponins of fermented Radix Astragali. The extraction method comprises the following steps: 1, carrying out warm immersion extraction on the fermented Radix Astragali by 80-90% ethanol, preferably 85% ethanol in order to obtain a fermented Radix Astragali alcohol extract fluid extract; 2, extracting the fermented Radix Astragali alcohol extract fluid extract obtained in step 1 by water saturated n-butanol in order to obtain an extract liquid; and 3, adding the extract liquid into a macro-porous adsorption resin column for absorption, euting, collecting the obtained eluate, and post-processing the eluate to obtain purified total saponins of the fermented Radix Astragali. The purity of saponins is further improved through the extraction method, and reaches 86-92%.

Description

technical field [0001] The invention belongs to the technical fields of bioengineering and medicine, and in particular relates to a preparation method of fermented astragalus membranaceus and an extraction method of total saponins. Background technique [0002] Radix Astragalus (Radix Astragalus) is the dried root of the leguminous plant Astragalus mongolica or Astragalus membranaceus. Sweet in taste, slightly warm in nature, has the effect of replenishing qi and tonifying deficiency. Astragalus total saponins include more than a dozen saponins, which have antibacterial, anti-inflammatory, anti-tumor, blood pressure-lowering, anti-myocardial hypoxia, central analgesic, and sedative effects. They are the main active ingredients of Astragalus, and their content in crude drugs is 0.095%~0.223%. Due to the low content, on the one hand, researchers continue to develop the efficient separation and extraction technology of total astragalosides, and on the other hand, they are com...

Claims

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Application Information

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IPC IPC(8): C12P1/04A23K1/16A61K36/481C12R1/46A23K10/12
Inventor 秦哲李建喜杨志强王学智张景艳王旭荣王磊孔晓军孟嘉仁张凯
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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