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Bio-organic fertilizer containing highly cadmium-resistant strains and its preparation method and application

A technology of bio-organic fertilizer and organic fertilizer, which is applied in the field of agricultural microorganism application, can solve the problems of difficult large-scale promotion and large funds required for repair, and achieves strong operability, reduced bioavailability, and advanced and scientific preparation methods Effect

Inactive Publication Date: 2018-08-24
HUNAN RANSUN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latter is difficult to carry out large-scale promotion due to the large funds required for engineering restoration

Method used

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  • Bio-organic fertilizer containing highly cadmium-resistant strains and its preparation method and application
  • Bio-organic fertilizer containing highly cadmium-resistant strains and its preparation method and application
  • Bio-organic fertilizer containing highly cadmium-resistant strains and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Contains the composition of the bio-organic fertilizer of highly cadmium-resistant strain

[0024] It consists of the following components in parts by weight: 5 parts of core bacterial agents; 2 parts of ion antagonists; and 93 parts of refined organic fertilizers.

[0025] The core bacterial agent is that Rhodopseudomonas palustris-RP3, which is highly resistant to cadmium pollution, is cultured on a slant, a shaking table, and a fermenter in sequence under sterile conditions, and the obtained fermentation broth is concentrated and dried to obtain spore powder. , and then mix spore powder, enzyme active substance and bentonite according to the weight ratio of 1:1:8, and the effective number of viable bacteria is 50.0×10 8 cfu / g.

[0026] Wherein said microbial strain is the dominant bacterial strain specially screened out from the sludge of the paddy field fish pond of the cadmium-contaminated area of ​​Datangpo, Tianyuan District, Zhuzhou City, named aft...

Embodiment 2

[0041] Embodiment 2 Contains the composition of the bio-organic fertilizer of highly cadmium-resistant strain

[0042] It consists of the following components in parts by weight: 5 parts of core bacterial agent; 1 part of ion antagonist; 94 parts of refined organic fertilizer.

[0043] The core bacterial agent is that the Rhodopseudomonas palustris-RP3 with high resistance to cadmium pollution in Example 1 is successively cultivated on a slant, a shaker, and a fermenter under aseptic conditions, and the fermented liquid obtained is passed through Concentrate and dry to obtain spore powder, then mix spore powder with enzyme active substance and bentonite according to the weight ratio of 1:1:8, and the effective number of viable bacteria is 55.0×10 8 cfu / g.

[0044] Wherein the enzyme active substance is protein powder and active enzyme, which is prepared by uniformly mixing protein powder: active enzyme at a weight ratio of 300:1 to 100:1, and the active enzyme is extracted fr...

Embodiment 3

[0059] Embodiment 3 Containing the composition of the bio-organic fertilizer of highly cadmium-resistant strain

[0060] It consists of the following components in parts by weight: 6 parts of core bacterial agents; 3 parts of ion antagonists; and 91 parts of refined organic fertilizers.

[0061] The core bacterial agent is that the Rhodopseudomonas palustris-RP3 with high resistance to cadmium pollution in Example 1 is successively cultivated on a slant, a shaker, and a fermenter under aseptic conditions, and the fermented liquid obtained is passed through Concentrate and dry to obtain spore powder, and then mix spore powder with enzyme active substance and bentonite according to the weight ratio of 1:1:8, and the effective number of viable bacteria is 40.0~60.0×10 8 cfu / g.

[0062] Wherein the enzyme active substance is protein powder and active enzyme, which is prepared by uniformly mixing protein powder: active enzyme at a weight ratio of 300:1 to 100:1, and the active enz...

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Abstract

The invention provides a biological organic fertilizer with high-cadmium-pollution-resistance bacterial strains. The biological organic fertilizer is composed of, by weight, 4 parts to 6 parts of core microbial agents, 1 part to 3 parts of ion antagonists, and 91 parts to 95 parts of refined organic fertilizers. The invention further provides production and application of the biological organic fertilizer with the high-cadmium-pollution-resistance bacterial strains. The microorganism bacterial strains in the core microbial agents are dominant strains which are specially screened out of sludge of rice field fishponds in cadmium pollution areas. The microorganism bacterial strains are powerful in function and can rapidly grow in soil in a field planting mode to form strong bacterial colonies. The chemical form of cadmium in soil is changed through the live and multiply effect of the microorganism bacterial strains so that the cadmium can be fixed or passivated or detoxified, and the mobility and the bio-availability of cadmium in the soil environment are reduced. The bacterial strains can directly adsorb heavy metal ions Cd+ to microbial cell walls or cells for metabolism, and the cadmium is reduced, purified and fixed through the combined action of the ion antagonists and the refined organic fertilizers.

Description

technical field [0001] The invention belongs to the application field of agricultural microorganisms, and in particular relates to a bio-organic fertilizer containing an active bacterial agent and a preparation method and application thereof. Background technique [0002] With the development of industrialization and agricultural intensification economy, the heavy metal pollution of cultivated land in my country is becoming more and more serious. In the early 1990s, the area of ​​cultivated land polluted by cadmium in my country reached 1.3×10 4 hm 2 , involving 25 regions in 11 provinces and cities. According to the special investigation of heavy metal pollution in Hunan Province and the monitoring results of agricultural environment quality in recent years, the cultivated land area polluted by cadmium in Hunan Province has accounted for 23.7% of the total cultivated land area in the province, and about 27% of farmland irrigation water and 25% The atmosphere of the left ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/40C05G3/04C09K101/00C05G3/80
Inventor 唐毓杰胡九洲孟三宏刘海涛杨丽
Owner HUNAN RANSUN BIOTECH
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