Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing organophosphorus

A technology of molecular imprinting and imitating enzymes, which is applied in the fields of alkali metal compounds, chemical instruments and methods, and inorganic chemistry. It can solve the problems of too deep embedding of template molecules, difficulty in removing template molecules, and low activity of imitating enzymes, and achieve low cost. , large surface area, large adsorption capacity

Inactive Publication Date: 2014-11-12
SHAANXI NORMAL UNIV
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the functional monomer 4(5)-vinylimidazole used in this method is not commercially available and needs to be synthesized from urocanic acid. also lower cost
In addition, the polymer mimetic enzyme prepared by the bulk polymerization method has large particles, and the catalytic active center is embedded in the polymer. Due to the influence of mass transfer resistance, the activity of the mimetic enzyme is low. Grinding, crushing and sieving of polymer imitating enzymes is not only a cumbersome process, but also a large number of irregular particles will be produced during the grinding process, and the superfine particles after sieving need to be removed, which is labor-intensive and time-consuming, and the yield is greatly improved. Decline (generally less than 50%)
In addition, due to the high concentration of the cross-linking agent, the resulting polymer is highly cross-linked, resulting in too deep embedding of the template molecules, making the removal of the template molecules very difficult.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing organophosphorus
  • Method for preparing mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing organophosphorus
  • Method for preparing mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing organophosphorus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 0.1530g (1.125mmol) ZnCl 2 , 96 μL (1.125 mmol) methacrylic acid, 408 μL (4.5 mmol) 1-vinylimidazole and 99 μL (0.45 mmol) (4-nitrobenzyl) diethyl phosphate were dissolved in 80 mL of acetonitrile and methanol with a volume ratio of 9: 1, add 2.4mL (16.8mmol) divinylbenzene and 0.2624g (1.6mmol) 2,2'-azobisisobutyronitrile, and then use a wavelength of 365nm under nitrogen protection and room temperature Ultraviolet light irradiation for 12 hours, then centrifuged at 8000 rpm for 10 minutes with a centrifuge, and the resulting solid was shaken and washed with 100 mmol / L bipyridyl methanol solution for 7 hours to remove (4-nitrobenzyl) diethyl phosphate ester, and then washed 3 times with methanol, each time for 30 minutes, and then washed with 100mmol / L ZnCl 2 The methanol solution was incubated for 4 hours, centrifuged at 8000 rpm for 10 minutes, the supernatant was discarded, and vacuum-dried at 45°C to obtain molecularly imprinted polymer microspheres of organophosp...

Embodiment 2

[0025] 0.1224g (0.9mmol) ZnCl 2 , 77 μL (0.9 mmol) of methacrylic acid, 326 μL (3.6 mmol) of 1-vinylimidazole and 99 μL (0.45 mmol) of (4-nitrobenzyl) diethyl phosphate were dissolved in 50 mL of acetonitrile and methanol at a volume ratio of 9: 1, add 1.9mL (13.5mmol) divinylbenzene and 0.2214g (1.35mmol) 2,2'-azobisisobutyronitrile, and then under nitrogen protection and room temperature, use a wavelength of 365nm Ultraviolet light irradiation for 12 hours, then centrifuged at 8000 rpm for 10 minutes with a centrifuge, and the resulting solid was shaken and washed with 100 mmol / L bipyridyl methanol solution for 7 hours to remove (4-nitrobenzyl) diethyl phosphate ester, and then washed 3 times with methanol, each time for 30 minutes, and then washed with 100mmol / L ZnCl 2 The methanol solution was incubated for 4 hours, centrifuged at 8000 rpm for 10 minutes, the supernatant was discarded, and vacuum-dried at 45°C to obtain molecularly imprinted polymer microspheres of organo...

Embodiment 3

[0027] 0.1836g (1.35mmol) ZnCl 2 , 115 μL (1.35 mmol) of methacrylic acid, 612 μL (6.75 mmol) of 1-vinylimidazole and 99 μL (0.45 mmol) of (4-nitrobenzyl) diethyl phosphate were dissolved in 100 mL of acetonitrile and methanol at a volume ratio of 9: 1, add 3.2mL (22.5mmol) of divinylbenzene and 0.3690g (2.25mmol) of 2,2'-azobisisobutyronitrile, and then use a wavelength of 365nm under nitrogen protection and room temperature Ultraviolet light irradiation for 24 hours, then centrifuged at 8000 rpm for 10 minutes with a centrifuge, and the resulting solid was shaken and washed with 100 mmol / L bipyridyl methanol solution for 7 hours to remove (4-nitrobenzyl) diethyl phosphate ester, and then washed 3 times with methanol, each time for 30 minutes, and then washed with 100mmol / L ZnCl 2The methanol solution was incubated for 4 hours, centrifuged at 8000 rpm for 10 minutes, the supernatant was discarded, and vacuum-dried at 45°C to obtain molecularly imprinted polymer microspheres ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing a mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing organophosphorus. A paraoxon transient state analogue (4-nitro benzyl) phosphoric acid diethyl ester serves as a template molecule, 1-vinyl imidazole and methacrylic acid are functional monomers, a molecular imprinting technology is used, a precipitation polymerization method is adopted, and therefore the mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing the organophosphorus is prepared, wherein the mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing the organophosphorus is good in dispersibility and uniform in particle size. The paraoxon hydrolytic enzyme activity of the prepared molecularly imprinted polymer microsphere and the paraoxon spontaneous hydrolyzing are compared, the hydrolyzing efficiency can be improved by 188 times to the maximum and is also improved by 2.3 times compared with the catalyzing efficiency of a non-imprinted polymer synthesized under the condition of not adding template molecules, a measured Km value and a measured Vmax value are 0.064 mmol / L min and 2.41 mmol / L respectively, and Kcat is 0.237 / S. The prepared mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing the organophosphorus can be used for efficiently hydrolyzing organophosphorus pesticide and can also be used for degrading and destroying organophosphorus nervous toxic agent chemical weapons.

Description

technical field [0001] The invention belongs to the technical field of degradation of organophosphorus compounds, and in particular relates to a method for preparing molecularly imprinted polymer microspheres of organophosphorus hydrolysis simulating enzymes. Background technique [0002] Organophosphorus pesticides are widely used because of their unstable chemical properties, easy decomposition, short half-life, and difficult accumulation in crops, animals and humans. However, since organophosphorus is used in a wider and wider range, the frequency of use is higher and higher, and the amount of application is increasing, it has become the most serious problem of food contamination. At the same time, organophosphorus compounds can also be used as nerve agents in wars and terrorist attacks. In order to effectively control organophosphorus pesticide residues and organophosphorus chemical weapons, in addition to strengthening the management and restriction of pesticides and n...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08F226/06C08F220/06C08F212/36C08F2/48C08J9/26B01J20/26B01J20/28B01J20/30
Inventor 张耀东马珂珂徐吉秀岳宣峰张林李梦凡王丽慧
Owner SHAANXI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com