Duck herpesvirus type 1 ul7 truncated recombinant protein and its preparation method and application

Abstract

The invention discloses an anatid herpesvirus 1 (AHV-1) UL7 truncated recombinant protein shown in SEQ ID No.2, as well as a code gene, a recombinant expression vector and engineering bacteria of the AHV-1 UL7 truncated recombinant protein, and a method for preparing the truncated recombinant protein and a polyclonal antibody of the truncated recombinant protein from the engineering bacteria. The truncated recombinant protein is similar to a natural AHV-1 UL7 protein in immunoreactivity, can be specifically bound with an AHV-1 antibody to be served as a reagent for detecting the AHV-1 antibody. As the truncated recombinant protein is not a complete bacteria, the risk of poison diffusion can be avoided during detection; the preparation method is simple, and suitable for industrialized mass production; product standardization can be realized; comparison among results of different laboratories can be facilitated; the polyclonal antibody of the truncated recombinant protein can serve as a reagent for detecting an AHV-1 antigen, is high in specificity, cannot react with duck hepatitis virus and new type gosling enteritis virus in a crossed manner, and can serve as reagent application for positioning and distributing the UL7 protein in an AHV-1 infected host cell.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a virus truncated recombinant protein and its preparation method and application. Background technique [0002] Anatid Herpesvirus 1 (AHV-1) belongs to Herpesviridae, α-Herpesvirinae, and Marekvirus genus, which can cause an acute, febrile, contact-infected infection in waterfowl such as ducks, geese, and swans. Bloody infectious disease, the clinical manifestations of sick ducks are depression, high fever, ataxia, diarrhea, tearing and swelling of the head and neck of some sick ducks. The main clinical feature is the process of acute sepsis. The main habitat of wild waterfowl spreads rapidly, and the morbidity and mortality can be as high as 90%. It often causes serious economic losses and seriously threatens the survival and development of my country's waterfowl breeding industry. AHV-1 antigen or antibody detection is critical to its prevention and treatment. The main met...

AI Technical Summary

Problems solved by technology

However, the previous ELISA method uses the whole virus as the coating antigen. The purity of the whole virus antigen is limited, the stability is poor, and it is difficult to standardize, which affects the accuracy of the test results. The operation is time-consuming and laborious, which further limits the large-scale application of this method. Therefore, The preparation method of a new type of antigen is particularly important for the establishment of a specific and sensitive detection method to prevent and control AHV-1 infection

Similarly, antigen detection methods include neutralization test, immunofluorescence, etc., but the detection reagents also involve highly specific antibodies, and the preparation of antibodies (whether monoclonal or polyclonal antibodies) also involves the difficulty of purifying viral antigens

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