Culturing device, culturing liquid and culturing system for external fertilization of human ovums and sperms and early development of embryo

Abstract

The invention discloses a culturing device, a culturing liquid and a culturing system for the external fertilization of human ovums and sperms and the early development of an embryo. The culturing device comprises at least two thermostatic culturing chambers, two small-sized air bottle chambers, a mixed gas filtering device, an air pressure protecting device, a mixed gas thermostatic preheating chamber, an air supplying pipe and the like, wherein each culturing chamber is a confined space with the volume of at least 100ml. The culturing liquid contains sodium chloride, magnesium sulfate, monopotassium phosphate, sodium bicarbonate, ethylenediamine tetraacetic acid, taurine, sodium pyruvate, L-lactic sodium lactate, glucose, gentamicin, alanine-glutamine dipeptide, 18 types of L-lactic amino acids except alanine and glutamine, and the like. When the culturing device and the culturing liquid are used, the external fertilization of the human ovums and sperms and the whole process of the development of the embryo before embryo implantation can be finished; the high-quality blastocyst forming rate and the embryo implantation rate are obviously increased.

Description

technical field [0001] The invention relates to a culture device and a culture system for in vitro fertilization of human eggs and sperm and early embryo development. Background technique [0002] Human in vitro fertilization-embryo transfer technology (In Vitro-Fertilization and Embryo Transfer, IVF-ET) has been developed for more than 30 years and has made great progress. The current in vitro fertilization and embryo culture are all completed in a carbon dioxide incubator, relying on the interaction and communication between the constant temperature and constant concentration of carbon dioxide in the incubator and the chemical components in the medium to maintain the sperm and egg fertilization and embryo growth. physiological environment. However, there are some defects or deficiencies in the existing culture system, whether in the hardware equipment (cultivation device or incubator) or in the culture medium. As far as the hardware is concerned, the existing culture dev...

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Problems solved by technology

As far as the hardware is concerned, the existing culture device (incubator) is an open system. The gas in the incubator needs to communicate with the air outside the case, that is, the air in the embryo laboratory to maintain the gas in the incubator or in the device. Balance and stability, so such an open system has the following obvious deficiencies or defects: First, the gas concentration and temperature in the device are actually in a dynamic equilibrium state, and the gas concentration and temperature fluctuate within a certain range. The size depends on the type and quality of the device or incubator; second, volatile organic compounds and harmful physical and chemical factors such as ozone in the laboratory can enter the incubator through this open gas exchange system, resulting in embryonic The third is that when the door of the incubator is opened, a large amount of gas in the incubator overflows, which inevitably causes the gas concentration and temperature in the incubator to fluctuate violently. The degree of damage also increases

In addition to the increase in the cost of the increase in the types of culture media, the main defect is that different media need to be replaced in a timely and accurate manner in different stages of insemination, cleavage and blastocyst culture. Unavoidable, which not only increases the number of times the door of the incubator is opened, resulting in a decrease in the culture effect, but also increases the risk of culture failure due to the use of the wrong medium type

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