Edible fungus liquid seed submerged fermentation packaged seed production technology and medium formulas thereof
A medium formula and deep fermentation technology are applied in the field of complete seed production technology of edible fungus liquid seeds and the medium formula field, which can solve the problems of difficult raw material processing, low utilization rate, inconvenient observation and the like
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Embodiment 1
[0058] The seed production process of the medium formula of embodiment 1—Pleurotus eryngii liquid seed complete set of seed production
[0059] A, Pleurotus eryngii test-tube seed medium formula and its seed production process
[0060] formula
[0061] Potato 300g boiled in 1500ml distilled water, glucose 30g, peptone 5g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, calcium chloride 0.5g, vitamin B1 powder 100mg, agar powder 20g
[0062] Test tube seed production process
[0063] A1. Slice 300g of potatoes, pour into 1500ml of distilled water to cook the juice, take the potato filtrate for later use; dissolve 30g of glucose, 5g of peptone, 2g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 0.5g of calcium chloride, and vitamin B1 powder into the potato filtrate 100 mg, stirred until fully dissolved to obtain a test tube culture medium premix;
[0064] A2. Pour the test tube culture medium premix back into the stainless steel pot, dissolve 20g of agar...
Embodiment 2
[0103] Embodiment 2—the seed production process of the medium formula of the complete set of seed production of shiitake mushroom liquid seeds
[0104] A. Medium formula and seed production process of Lentinus edodes in test tube
[0105] In the present embodiment, the formula of the test-tube seed culture medium of Lentinus edodes and the seed production process thereof are the same as the formula of the test-tube seed culture medium of Pleurotus eryngii and the seed production process thereof in Example 1.
[0106] B. Shake flask seed culture medium formula and seed production process of shiitake mushrooms
[0107] formula
[0108] EXAMPLES Test group: 15g of brown sugar, 15g of glucose, 5g of potato starch, 5g of peptone, 1g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of yeast extract, 0.3g of calcium chloride, and 0.5g of sodium hydroxide.
[0109] Control group 1: Glucose 30g, bran 10g, peptone 5g, potassium dihydrogen phosphate 1g, magnesium su...
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