Application of dimethyl sulfoxide to bacterial infection control
A technology of dimethyl sulfoxide and bacterial infection, applied in the direction of antibacterial drugs, sulfur/selenium/tellurium active ingredients, etc., can solve the problems of unreported application of DMSO, and achieve the solution of bacterial drug resistance and avoid drug resistance. Effect
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Embodiment 1
[0028] Embodiment 1: the construction of bacterial anti-pathogenic substance detection system
[0029] The specific principle of the detection system is: usually the strength of gene expression depends on the strength of the promoter of the gene itself, and the promoter of the toxicity-related gene is connected to the front of the reporter gene luciferase gene luxCDABE without promoter, so as to start the expression of the reporter gene . If the drug to be tested has an inhibitory effect on the promoter of the virulence factor, the expression of the reporter gene will also be weakened, that is, the luminescence of the bacteria will be weakened; on the contrary, if the drug has an activation effect on the promoter, the luminescence of the bacteria will be enhanced. The effect of DMSO on the expression of virulence factors can be known from the changes in the apparent luminescence of the bacteria.
[0030] The toxicity factors of the detection system used in the research includ...
Embodiment 2
[0032]Example 2: Effect of DMSO on the expression of Pseudomonas aeruginosa virulence factors
[0033] Through the experimental results, it was found that DMSO with a concentration below 10% had no obvious inhibitory effect on the growth of Pseudomonas aeruginosa. The embodiment takes 2% concentration as an example to study the effect of DMSO on the expression of various virulence factors without affecting the growth of bacteria.
[0034] Specifically, the gene expression detection was carried out by an enzyme label detector: the detection strain was streaked on LB solid medium and cultured overnight at 37°C. A single clone was picked and inoculated into a white 96-well plate containing 100 μL LB medium, and cultured at 37° C. and 200 rpm for 12 hours. Then transfer to fresh LB medium according to 5% inoculum amount, activate for 3 hours, take 95 μL of fresh LB containing 2% (v / v) DMSO or without DMSO (control group) to culture based on sterilized black transparent bottom In...
Embodiment 3
[0040] Example 3: Detection of the impact of DMSO on toxicity-related phenotypes of Pseudomonas aeruginosa
[0041] From the above detection results of DMSO on the expression of toxic genes, it was found that DMSO had a significant inhibitory effect on the expression of synthetic genes of various toxic factors. On this basis, the influence of DMSO on the production of related toxic factors was further tested.
[0042] 3.1 Inhibitory effect of DMSO on pyocyanin production of Pseudomonas aeruginosa
[0043] Pyocyanin (PYO) is an important phenazine compound secreted by Pseudomonas aeruginosa, which can regulate the redox state of cells, and can also act as a signal molecule to regulate some pathogenicity of Pseudomonas aeruginosa The expression of related genes plays an important role in the infection process it initiates. like figure 1 , figure 2 As shown, DMSO has a very obvious inhibitory effect on the expression of toxic genes phzA1 and phzA2. Therefore, this embodiment...
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