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Protein-grafted natural polysaccharide, preparation method and application thereof

A technology of protein and protein solution, which is applied in the field of protein grafting natural polysaccharide, fish skin collagen and its hydrolyzate grafted chitosan oligosaccharide and its preparation, which can solve the problems of rare animal protein and polysaccharide, toxic and side effects of reaction intermediates, reaction Long time and other problems, to achieve the effect of good moisture absorption, good anti-oxidation, mild reaction conditions

Active Publication Date: 2014-08-13
湖南尔康明胶有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Most of the above-mentioned prior art discloses grafting of plant protein and polysaccharide, but reports on grafting of animal protein and polysaccharide are relatively rare, and the preparation method described in the prior art also has long reaction time, high cost, Reaction intermediates have toxic side effects, etc.

Method used

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  • Protein-grafted natural polysaccharide, preparation method and application thereof
  • Protein-grafted natural polysaccharide, preparation method and application thereof
  • Protein-grafted natural polysaccharide, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Fish skin collagen graft chitosan oligosaccharide, its preparation method is as follows:

[0036] (1) Prepare a phosphate buffer solution with a pH of 5.0, measure 15 ml of phosphate buffer solution into a three-necked flask, add 1.0 g of chitosan oligosaccharides, and dissolve it fully; and dissolve 0.4 g of fish skin collagen in 25 ml of phosphate buffer in solution;

[0037] (2) Catalyzed grafting: Add the fish skin collagen buffer solution obtained in step (1) and 0.10 g transglutaminase to the chitosan oligosaccharide solution obtained in step (1), and stir magnetically at 10°C for 1 Hour;

[0038] (3) Post-treatment: continue to heat up the solution obtained in step (2) to 100°C, and stir it magnetically for 10 minutes, then filter it with suction, purify it by dialysis for three days, and freeze-dry it to obtain fish skin collagen grafted chitosan oligosaccharide .

[0039] It is determined that the substitution degree of the amino group in the fish skin colla...

Embodiment 2

[0041] Hydrolyzed collagen grafted chitosan oligosaccharide, its preparation method is as follows:

[0042] (1) Prepare a phosphate buffer solution with a pH of 6.0, measure 20 ml of the phosphate buffer solution into a three-necked flask, add 1.0 g of chitosan oligosaccharide, and dissolve it fully; another 0.8 g of collagen is hydrolyzed by alkaline protease The obtained hydrolyzed collagen was dissolved in 25 ml of phosphate buffer solution;

[0043] (2) Catalyzed grafting: Add the hydrolyzed collagen buffer solution obtained in step (1) and 0.15 g of transglutaminase to the chitosan oligosaccharide solution obtained in step (1), and stir magnetically at 20°C for 3 hours ;

[0044] (3) Post-treatment: continue to heat up the solution obtained in step (2) to 100°C, and stir it magnetically for 10 minutes, then filter it with suction, purify it by dialysis for three days, and freeze-dry to obtain the hydrolyzed collagen grafted chitosan oligosaccharide.

[0045] It is deter...

Embodiment 3

[0047] Fish gelatin grafted chitosan oligosaccharide, its preparation method is as follows:

[0048] (1) Prepare a phosphate buffer solution with a pH of 7.0, measure 25 ml of the phosphate buffer solution into a three-necked flask, add 1.0 g of chitosan oligosaccharide, and dissolve it fully; Gelatin was dissolved in 25 ml phosphate buffer solution;

[0049] (2) Catalyzed grafting: Add the gelatin buffer solution obtained in step (1) and 0.20 g of transglutaminase to the chitosan oligosaccharide solution obtained in step (1), and stir magnetically at 40°C for 6 hours;

[0050] (3) Post-treatment: continue to heat up the solution obtained in step (2) to 100°C, and stir it magnetically for 10 minutes, then filter it with suction, purify it by dialysis for three days, and freeze-dry to obtain fish gelatin-grafted chitosan oligosaccharide.

[0051] It is determined that the degree of substitution of amino groups in the fish scale collagen grafted chitosan by fish gelatin is 0.52...

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Abstract

The invention discloses protein-grafted natural polysaccharide, a preparation method and an application thereof, and concretely relates to bio-protein-grafted chitosan oligosaccharide, a preparation method and an application, which belongs to the high-molecular modification field. The bio-protein-grafted chitosan oligosaccharide is obtained by dissolved chitosan oligosaccharide and a bio-protein solution under effect of a catalyst microbial transglutaminase. According to the invention, reaction condition is mild, technology is simple, and the reaction substances are natural macromolecule with characteristics of environmental protection. The prepared water-soluble chitosan oligosaccharide bio-protein copolymer has good film forming ability, moisture-absorption effect, moisture-retention effect and antioxidation effect.

Description

technical field [0001] The invention belongs to the field of natural macromolecule modification, and specifically relates to a protein grafted with natural polysaccharides, in particular to fish skin collagen and its hydrolyzate grafted with chitosan oligosaccharides, a preparation method and an application thereof. Background technique [0002] Protein and polysaccharide are the two most important types of biomacromolecules that coexist in the food emulsification system, and are the main factors affecting the structure and texture of food. Proteins are often used as emulsifiers in colloidal systems because they can form an adsorption layer on the liquid-liquid or gas-liquid interface to reduce interfacial tension. Polysaccharides are often used as stabilizers due to their good thickening and water-holding properties, thus endowed The system is different from the functional performance of the two alone. Covalently bonded proteins and polysaccharides form grafts, which not o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/00C08B37/08C08G81/00
Inventor 帅放文王向峰章家伟
Owner 湖南尔康明胶有限公司
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