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A freshwater chlorella sorokiniana GS03 and its application

A technology of chlorella, CCTCCM2013607, applied in the field of biomass energy, can solve the problem of high production cost of microalgae biodiesel, achieve high carbon dioxide fixation efficiency, biomass yield and oil yield, and high carbon dioxide tolerance , the effect of efficient use

Inactive Publication Date: 2016-03-02
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the defects of the prior art, to provide a strain of freshwater chlorella with advantages, which can convert industrial flue gas CO 2 The raw material for biodiesel preparation, this invention can effectively solve the problem of the current treatment of air pollutants from industrial sources while coupling the production of microalgae oil to reduce the high cost of microalgae biodiesel production

Method used

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  • A freshwater chlorella sorokiniana GS03 and its application
  • A freshwater chlorella sorokiniana GS03 and its application
  • A freshwater chlorella sorokiniana GS03 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Under sterile conditions, single algae were picked from a solid plate and dropped into a 100ml Erlenmeyer flask containing 30ml sterilized BG-11 culture solution, and cultured statically in a light culture rack at a temperature of 28°C and a light intensity of 7500lux for 12 days. Grow to the end of the index and expand the culture with an inoculation ratio of 1:10.

[0050] Prepare 2.4L of BG-11 medium, insert the expanded cultured cells, and inoculate the cells with optical density OD 680 = 1.2. Initial pH 4.0, temperature 15°C, light-to-dark ratio 8:16, 0.5vvm air (CO 2 The content is 0.03% (v / v)), cultured for 14 days.

[0051] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the algal powder was weighed and the dry weight was calculated. The biomass concentration of algal powder is 680mg / L, the biomass production rate is 70mg / L / d, CO 2 The fixation efficiency is 110mg / L / d, and the oil content measured by the chloroform-metha...

Embodiment 2

[0053] Pick a single algae on a solid plate under sterile conditions and drop it into a 100ml Erlenmeyer flask containing 30ml of sterilized culture solution, and place it in a light culture rack for static culture. The temperature is 28°C, and the light intensity is 7500lux. Cultivate for 12 days and grow to an index. At the end of the period, expand the culture with a 5% inoculation ratio.

[0054] Prepare 3.1L of BG-11 medium and insert it into the expanded cultured cells. The initial pH is 7.0, the temperature is 45°C, the light-to-dark ratio is 24:0, under the light intensity of 2500lux, 1vvm of 30% CO is introduced 2 (v / v), cultured for 10 days.

[0055] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the dry weight of the algal powder was weighed and calculated. The biomass concentration of algae powder is 2400mg / L, the biomass production rate is 360mg / L / d, CO 2 The fixation efficiency is 660mg / L / d, the oil content determined by ...

Embodiment 3

[0057] Pick a single algae on a solid plate under sterile conditions and drop it into a 100ml Erlenmeyer flask containing 30ml of sterilized culture solution, and place it in a light culture rack for static culture. The temperature is 28°C, and the light intensity is 7500lux. Cultivate for 12 days and grow to an index. In the final stage, the inoculation ratio was 1:10 to expand the culture.

[0058] Prepare 3.1L of BG-11 medium and insert it into the expanded cultured cells. Initial pH 11.0, temperature 10°C, light-to-dark ratio 12:12, 45% CO at 0.2vvm under light intensity of 9500lux 2 (v / v), cultured for 14 days.

[0059] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the dry weight of the algal powder was weighed and calculated. The biomass concentration of algae powder is 2500mg / L, the biomass production rate is 260mg / L / d, CO 2 The fixation efficiency is 480mg / L / d, and the oil content determined by the chloroform-methanol method i...

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Abstract

The invention relates to the technical field of biomass energy, and in particular to a freshwater chlorella strain (i) Chlorella sorokiniana ( / i) GS03 and application thereof to industrial flue gas CO2 fixation and production of microalgae biodiesel. The chlorella strain (i) C.sorokiniana ( / i) GS03 provided by the invention has a preservation number of CCTCC M2013607, and higher CO2 tolerance, higher fixation efficiency of high-concentration carbon dioxide and higher biomass yield and oil yield compared with the reported microalgae strains. Therefore, the invention can greatly reduce microalgal oil production cost, and provides a good production strain for algae for efficient fixation of CO2 in typical industrial flue gas environment and biodiesel production from microalgae oil, and shows great application prospect in the field of biomass energy.

Description

technical field [0001] The invention relates to the technical field of biomass energy, in particular to a freshwater chlorella ChlorellasorokinianaGS03 and its use in industrial flue gas CO 2 Application of immobilization and production of microalgae to biodiesel. Background technique [0002] Due to the extensive use of fossil fuels in the world, the "greenhouse effect" is increasing, and the CO caused by the burning of fossil fuels 2 It accounts for more than 65% of the total "greenhouse effect" gases. Therefore, petrochemical, smelting, electric power and other industries CO 2 Emission reduction has become the focus of sustainable development. [0003] In recent years, some developed countries have used various physical, chemical and biological methods to study CO 2 collection, concentration, fixation and transformation. But compared to traditional physical and chemical CO 2 The sequestration method has the characteristics of high cost, high energy consumption, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P7/64C11B1/00C10L1/02C12R1/89
CPCY02E50/10
Inventor 缪晓玲巩三强
Owner SHANGHAI JIAOTONG UNIV
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