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Gingko, ganoderma lucidum and cordyceps spirit and preparation method thereof

A technology of ginkgo and ganoderma, applied in the field of bioengineering, can solve problems such as hindering the consumption of ginkgo in large quantities, achieve good social benefits, and prevent cardiovascular and cerebrovascular diseases

Inactive Publication Date: 2014-07-16
董铸华 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to these side effects hinder the consumption of a large amount of ginkgo

Method used

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  • Gingko, ganoderma lucidum and cordyceps spirit and preparation method thereof
  • Gingko, ganoderma lucidum and cordyceps spirit and preparation method thereof
  • Gingko, ganoderma lucidum and cordyceps spirit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Accurately weigh 200g of ginkgo biloba that has been shelled, beat it, add 2000mL of water, boil and gelatinize, cool down to 80°C, add 100mL of liquefied enzyme (Wuxi Saide Biological Engineering Co., Ltd., 160,000 units / mL) 80 ℃ hydrolysis for 10 minutes, lower the temperature to 70 ℃, add 100mL glucoamylase (Wuxi Saide Biological Engineering Co., Ltd., 20,000 units / mL) hydrolysis for 10 minutes at 70 ℃, lower the temperature to 50 ℃, add 30g papain (Guangxi Nanning Donghenghua Road Biotechnology Co., Ltd., 800,000 units / g), hydrolyzed for 0.5 hour, made Ginkgo hydrolyzate;

[0032] (2) 1000mL PDA medium (potato dextrose agar medium) containing 200g potato extract (peeled potatoes cut into pieces, boiled in 1000mL distilled water for 30 minutes, filtered through eight layers of gauze, and the filtrate was adjusted to 1000mL, and the filtrate was the potato extract) Add 20 g of sucrose and 20 g of agar, boil until the agar is completely melted, divide into test tub...

Embodiment 2

[0037] (1) Accurately weigh 200g of shelled ginkgo ginkgo, beat it, add 1000mL of water, boil for gelatinization, cool down to 80°C, add 100mL of liquefied enzyme (Wuxi Saide Biological Engineering Co., Ltd., 160,000 units / mL) 80 ℃ hydrolysis for 10 minutes, lower the temperature to 70 ℃, add 100mL glucoamylase (Wuxi Saide Biological Engineering Co., Ltd., 20,000 units / mL) hydrolysis for 10 minutes at 70 ℃, lower the temperature to 50 ℃, add 30g papain (Guangxi Nanning Donghenghua Road Biotechnology Co., Ltd., 800,000 units / g), hydrolyzed for 0.5 hour, made Ginkgo hydrolyzate;

[0038] (2) 1000mL PDA medium (potato dextrose agar medium) containing 200g potato extract (peeled potatoes cut into pieces, boiled in 1000mL distilled water for 30 minutes, filtered through eight layers of gauze, and the filtrate was adjusted to 1000mL, and the filtrate was the potato extract) Add 20 g of sucrose and 20 g of agar, boil until the agar is completely melted, divide into test tubes, plug w...

Embodiment 3

[0043] 10000 parts of ginkgo ganoderma lucidum cordyceps wine obtained in Example 1 is mixed with 10000 parts of kudzu root extract, 40-150 parts of honey, and 20-220 parts of syrup.

[0044]

[0045] Verify Example 1

[0046] Sixty ICR mice were randomly divided into 5 groups, 12 in each group. The normal control group was fed at 25°C; the cold-shock control group,

[0047] Each group of cold-shocked ginkgo ganoderma lucidum and cordyceps wine group was placed in a light incubator at (4±2)°C from 8:00 to 12:00 every morning, and then taken out and placed back in an environment at 25°C. Day 16:00 gavage embodiment 1, embodiment 2 wine, dosage is 10mL / Kg body weight, and normal control group, cold shock control group gavage equivalent normal saline every day is made contrast; All groups are fed feed normally, drink tap water . After 14 days, the mice were anesthetized by intraperitoneal injection of 5 mL / Kg body weight of 20% ethyl carbamate, and the blood pressure was me...

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Abstract

The invention relates to gingko, ganoderma lucidum and cordyceps spirit and a preparation method thereof.In this experiment, gingko is hydrolyzed by adopting a double-enzyme method to obtain a gingko hydrolysate; the hydrolysate contains active compounds such as gingko flavone, terpenoid, amino acid, peptides and starch, wherein the content of amino acid and the peptides is more than or equal to 1%, and the content of the starch is more than or equal to 2%. The hydrolysate is inoculated with ganoderma lucidum and cordyceps strains for transforming and fermented by adding rice yeast to obtain fermentation broth, and the fermentation broth is filtered and blended to obtain a gingko, ganoderma lucidum and cordyceps spirit. The alcohol content is 8-16%, the content of total flavone is 1-5mg / L, the ginkgolide is 1-8mg / L, the content of total terpenoid is 20-600mg / L, the protein is 70-260mg / L, the ganoderic acid is 12-20mg / L, the soluble sugar is 0-3.2%, the cordycepic acid is 12-25mg / L, the cordycepin is 0.2-1.6mg / L, the ginkgolic acid is 0-4ppm, and the hydrocyanic acid is 0-0.16ug / L. The spirit containing the active ingredients of gingko, ganoderma lucidum and cordyceps has the effects of preventing hypertension, resisting oxidation, enhancing the immunity and the like.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a ginkgo ganoderma lucidum cordyceps wine and a preparation method thereof. Background technique [0002] Ginkgo biloba is a plant fruit with both medicinal and edible properties. It is rich in various valuable nutrients such as ginkgo biloba and ginkgo lactone. It has a good effect on improving human immunity, preventing and treating cardiovascular and cerebrovascular diseases. [0003] Ancient books of traditional Chinese medicine in my country have always listed ginkgo as an important medicinal material. Ginkgo acid inhibits a variety of bacilli and skin fungi, and has different effects on staphylococcus, streptococcus, diphtheria, anthrax, Bacillus subtilis, Escherichia coli, typhoid bacteria, etc. Degree of inhibitory effect o ginkgo acid extracted from fresh ginkgo fruit has a hypotensive effect and increases the permeability of blood vessels. In recent years, clinic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12G3/02C12G3/04
Inventor 董铸华房舒
Owner 董铸华
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