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Method for conveying microorganisms to stratum

A technology of microbes and microbial liquids, which is applied in the field of geological microbiology engineering applications, can solve the problems of low permeability reservoirs with low permeability, difficult microbial injection, and inability to achieve migration, so as to achieve broad application prospects and improve the effect of fracturing operations , the effect of flexible construction methods

Inactive Publication Date: 2014-07-09
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology mainly uses the oilfield water flooding process, adding microbial strains to the injected water, and using its characteristic metabolites such as acids, gases, biosurfactants, solvents, etc. to improve the development effect of low-permeability reservoirs. Reservoir permeability is low, conventional microbial injection is difficult, effective migration cannot be achieved, and bacteria filter cakes are more likely to be formed near the wellbore. Although this technology proposes that microbial oil recovery technology can be applied to fracturing wells, it does not specifically propose Practical ways to deliver bacteria using hydraulic fracturing

Method used

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  • Method for conveying microorganisms to stratum
  • Method for conveying microorganisms to stratum
  • Method for conveying microorganisms to stratum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation of spore solution : Bacillus subtilis ATCC21332 and Bacillus mohjavens ATCC39037 were selected according to Lin, S.C., Minton, M.A., Sharma, M.M., Georgiou, G.1994.Structural and immunological characterization of abisurfactant produced by Bacillus licheniformis JF-2. Appl Environ Microbiol, 60(1), 31-8. and Sun, H., Bie, X., Lu, F., Lu, Y., Wu, Y., Lu, Z. 2009.Enhancement of surfactin production of Bacillus subtilis fmbR by replacement of the native promoter with the Pspac promoter. Can J Microbiol, 55 (8), the method in 1003-6. cultivates vegetative cells, and the prepared vegetative cells are left standstill After 3 days to 10 days, the spore formation rate reached 95% and 98%, respectively, and placed in a water bath at 30°C to 100°C for 1min to 100min to kill residual vegetative cells. After cooling to room temperature, prepare 1.0 ×10 9 CFU / mL concentration of spore suspension.

Embodiment 2

[0051] Preparation of cell suspension during starvation phase : The vegetative cell culture solutions of Bacillus subtilis ATCC21332 and Bacillus mohjavens ATCC39037 were respectively prepared according to the method for culturing vegetative cells in Example 1. Collect the cells by centrifugation and resuspend them in sterile water, let them stand for different days to make the cells in the suspension enter the starvation period, and prepare 1.0×10 8 The starvation period cell suspension at the concentration of CFU / mL was compared with the normal vegetative somatic cell morphology under the microscope under the microscope, and the experimental results are shown in Table 1.

[0052] Table 1 Comparison of cell morphology between vegetative cells and starvation cells

[0053]

[0054] It can be seen from Table 1 that due to the lack of nutrition, the morphology of the microbial cells in the starvation period will change significantly, the lysates in the cells will decrease, ...

Embodiment 3

[0056] Anti-pressure performance experiment of microorganisms : Get the vegetative cell suspension, starvation period cell suspension and spore suspension of Bacillus subtilis (Bacillus subtilis ATCC21332) and Bacillus mohjavens (Bacillus mohjavens ATCC 39037) prepared respectively according to the method in Example 1 and 2 , respectively formulated as

[0057] 1.0×10 7 The suspension of CFU / mL bacterial cell concentration. These three kinds of suspensions were placed in intermediate containers respectively, pressurized to 60MPa and 80MPa respectively for 10 hours, and then taken out after treatment, and the concentration of viable bacteria was detected by the frequency dilution method (Most probable number, MPN). The experimental results are shown in Table 2 .

[0058] Table 2 Comparison results of the anti-compressive performance of bacteria under three cell forms

[0059]

[0060] It can be seen from the table that with the increase of the treatment pressure, the co...

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Abstract

The invention provides a method for conveying microorganisms to a stratum. The method is characterized in that microorganisms are carried in a viscous fluid and then go into the stratum by use of a hydraulic fracturing technology, wherein the microorganisms are pretreated so that the microorganisms are in at least one of the following existence forms: a vegetative cell suspension, a starvation-stage cell suspension and a spore suspension. The method realizes carrying the microorganisms deep into the stratum by use of the hydraulic fracturing technology; the microorganisms are appropriately pretreated so that higher conveying efficiency is achieved, and therefore, the microorganisms are enabled to be better in pressure resistance and migration capability, and higher in survival rate. The provided method is advantageous for guaranteeing that related engineering technical operations can be carried out deep into the stratum by using the microorganisms, and especially advantageous for utilizing the microorganisms in the fields of improving the production capacity of a fractured well, improving the oil recovery factor and the like.

Description

technical field [0001] The invention relates to a method for transporting microorganisms in the application field of geological microbiology engineering, and furthermore, relates to a method for transporting microorganisms into formations. Background technique [0002] Microbial oil recovery technology has the characteristics of low investment, economical and environmental protection, and long validity period. Its mechanism is generally believed to come from two aspects: the role of microbial cells themselves and the role of microbial metabolites. The former mainly includes the physical effects of microbial cells and the effect of microorganisms on Degradation of crude oil and other pollutants, the latter mainly including various metabolites of microorganisms, such as biological surface active substances, small molecule organic acids, gases, organic solvents, biopolymers, etc. The target of microbial metabolism and its metabolites can be formation fluid (formation water, cru...

Claims

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Application Information

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IPC IPC(8): E21B43/22E21B43/267
Inventor 郑承纲李宗田苏建政张汝生刘长印赵梦云黄志文孙志宇林鑫贺甲元杨科峰
Owner CHINA PETROLEUM & CHEM CORP
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