Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immobilization hydrolase as well as preparation method and application thereof

A technology of hydrolytic enzyme and amylase, applied in the direction of immobilized on or in inorganic carrier, fixed on/in organic carrier, biological sludge treatment, etc., can solve the problem of separation of immobilized hydrolytic enzyme and sludge, Poor stability, complex recycling and other problems, to achieve good application prospects, strong stability, simple and convenient recycling

Inactive Publication Date: 2014-07-02
CHANGSHA UNIVERSITY
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the sludge contains a large amount of organic particles, the use of hydrolytic enzymes immobilized by conventional organic carriers in the sludge hydrolysis process has disadvantages such as difficulty in separating the immobilized hydrolytic enzyme from the sludge, complicated recycling, and poor stability. Therefore, it is necessary to Find new ways to solve these technical problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] An immobilized hydrolase of the present invention is specifically an immobilized protease, which is prepared by cross-linking the protease on magnetic chitosan composite microspheres through glutaraldehyde adsorption, and the magnetic chitosan composite microspheres are by Fe 3 o 4 Nanoparticles and chitosan were cross-linked by glutaraldehyde.

[0033] A kind of preparation method of the immobilized protease of above-mentioned present embodiment, comprises the following steps:

[0034] 1. Preparation of Fe 3 o 4 nanoparticles

[0035] (1) Weigh 5.56g FeSO 4 ·7H 2O and 6.488g FeCl 3 , was dissolved in 200mL of ultrapure water to prepare iron salt solution, in which Fe 3+ and Fe 2+ The molar ratio is 2:1.

[0036] (2) use N 2 As a protective gas, under the condition of non-magnetic rapid stirring at room temperature, add ammonia water dropwise to the iron salt solution until the pH value is 10, and mature the product at 80°C for 30min to obtain Fe 3 o 4 susp...

Embodiment 2

[0049] An immobilized hydrolase of the present invention is specifically an immobilized amylase, which is prepared by cross-linking the amylase on magnetic chitosan composite microspheres through glutaraldehyde adsorption, and the magnetic chitosan composite The microspheres are made of Fe 3 o 4 Nanoparticles and chitosan were cross-linked by glutaraldehyde.

[0050] A kind of preparation method of the immobilized amylase of above-mentioned present embodiment, comprises the following steps:

[0051] 1. Preparation of Fe 3 o 4 nanoparticles

[0052] With the step 1 of embodiment 1.

[0053] 2. Preparation of Magnetic Chitosan Composite Microspheres

[0054] With the step 2 of embodiment 1.

[0055] 3. Preparation of immobilized amylase

[0056] (1) Dissolve 0.5g of industrial grade amylase (containing 45wt% impurities) in 9mL of phosphate buffer solution with a pH of 6.8 and a concentration of 0.1mol / L, and then add 1mL of sodium chloride solution with a mass concentrat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses immobilization hydrolase as well as a preparation method and application thereof. The immobilization hydrolase is prepared in a way that hydrolase is adsorbed and crosslinked on a magnetic chitosan composite microsphere by virtue of glutaraldehyde, and the magnetic chitosan composite microsphere is formed by crosslinking Fe3O4 nano particles with chitosan by virtue of glutaraldehyde. The preparation method of the immobilization hydrolase comprises the following steps: (1) adding Fe3O4 nano particles into a chitosan solution, and then adding a glutaraldehyde solution, so that a magnetic chitosan composite microsphere is obtained; (2), adding the magnetic chitosan composite microsphere into the glutaraldehyde solution for performing reaction, and adding the obtained carrier product into a hydrolase solution, so that the immobilization hydrolase is obtained. The immobilization hydrolase has strong stability on heat, strong acid, strong alkali, high ion strength, organic solvent and the like, is hardly inactivated and can be recycled. The immobilization hydrolase can be applied to an enhanced sludge hydrolysis process and can be used for solving the problems that an enzyme is used up in the enhanced sludge hydrolysis process and the enzyme can not be recycled.

Description

technical field [0001] The invention belongs to the field of sludge hydrolysis, and in particular relates to an immobilized hydrolytic enzyme and a preparation method and application thereof. Background technique [0002] Activated sludge method is one of the most widely used sewage biological treatment technologies in the world. The biggest drawback of this technology is the large amount of excess sludge. At present, my country's sludge production is about 25 million tons per year (calculated based on a moisture content of 85%). If it is not properly treated and disposed of in time, it will cause direct or potential pollution to the environment. Anaerobic digestion of sludge is a commonly used sludge treatment technology at home and abroad. Hydrolysis is the rate-limiting step of this process, and the poor degradability of excess sludge is the bottleneck problem restricting anaerobic digestion. Therefore, a technology to increase the hydrolysis rate of sludge is developed. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N11/14C12N11/10C02F11/04
Inventor 罗琨廖兴盛庞娅李雪裴习君
Owner CHANGSHA UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com