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Method and device for identifying and measuring phytoplankton based on discrete three-dimensional fluorescence spectroscopy

A phytoplankton and three-dimensional fluorescence technology, applied in the field of environmental science, can solve the problems of high operational stability, large volume, high power consumption, etc., and achieve high recognition and measurement capabilities, simple and compact structure, and easy to carry

Active Publication Date: 2016-04-20
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

However, due to the large size, high power consumption, and high requirements for operational stability of traditional three-dimensional fluorescence spectroscopy measuring instruments, the phytoplankton identification and determination method based on three-dimensional fluorescence spectroscopy remains at the stage of laboratory sample analysis, and it is difficult to meet the requirements of phytoplankton on-site. Identify assay needs

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  • Method and device for identifying and measuring phytoplankton based on discrete three-dimensional fluorescence spectroscopy
  • Method and device for identifying and measuring phytoplankton based on discrete three-dimensional fluorescence spectroscopy
  • Method and device for identifying and measuring phytoplankton based on discrete three-dimensional fluorescence spectroscopy

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Embodiment

[0045] Choose Microcystisaeruginosa (Microcystisaeruginosa), Chlorellavulgaris (Chlorellavulgaris), Fragilariasp (Fragilariasp) of the phylum Chlorophyta, Glenodiniumgymnodinium (Glenodiniumgymnodinium) and ovoid of Cryptophyta Cryptomonasovata (Cryptomonasovata) experimental algae species, take 15-day pure culture of the above five algae, use high performance liquid chromatography to measure the chlorophyll a concentration of each pure culture, and dilute the pure culture to a certain multiple with distilled water as a sample For the mother liquor, the chlorophyll a concentration of the sample mother liquor is calculated according to the dilution ratio, and the chlorophyll a concentration of the mother liquor is controlled in the range of 80-100μg / L. Then, the mother liquor of the five kinds of algae was diluted in half with distilled water into 3 pure samples of concentration gradient, and then mixed according to the volume ratio shown in Table 2 to prepare mixed samples of 26...

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Abstract

The invention discloses a discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement method and a discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement device. The measurement method comprises the following steps: selecting a characteristic fluorescence peak which corresponds to different photosynthetic pigments as an analytic target according to fingerprint characteristics of a phytoplankton fluorescence spectrum, maintaining the phytoplankton characteristic information in the three-dimensional fluorescence spectrum to the greatest degree, fitting a characteristic spectrum of the sample according to each characteristic spectrum in a standard discrete three-dimensional fluorescence spectrum library, and inverting the types and the content of the phytoplankton in the sample. According to the measurement device, a multiband light-emitting diode (LED) array serves as an excitation light source, and a multiband light filter group serves as a band selector for receiving fluorescence. The device is simple and compact in structure, small in size and convenient to carry and is suitable for identification and measurement of field phytoplankton, the phytoplankton identification and measurement method provided by the invention is combined, so that rapid classified measurement on the phytoplankton site can be realized.

Description

Technical field [0001] The invention relates to the field of environmental science, in particular to a method and device for identifying and measuring phytoplankton based on discrete three-dimensional fluorescence spectroscopy. Background technique [0002] The species and quantity of phytoplankton can reflect the ecological status of the area. Phytoplankton has been used as an indicator of water quality for a long time. Especially for large areas of water, the determination of the species and quantity of phytoplankton has more important practical significance for water quality evaluation. . With the eutrophication of inland lakes and offshore waters in my country, resulting in excessive proliferation of phytoplankton and frequent occurrence of red tides and water blooms, there is an urgent need to develop direct and rapid phytoplankton species and data analysis technologies and equipment for red tides and water China monitoring and early warning provide important means. [0003] ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 殷高方赵南京胡丽石朝毅余晓娅方丽段静波肖雪甘婷婷董欣欣张玉钧刘建国刘文清
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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