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Tea saponin bio-enzyme decoloring technology

A tea saponin and biological enzyme technology, applied in the field of tea saponin biological enzyme decolorization process, can solve the problems of loss and recovery of tea saponin, operator injury, environmental pollution, etc., and achieves low loss rate, mild reaction conditions, and decolorization time. short effect

Active Publication Date: 2014-06-18
HANGZHOU YIMIN PETOROCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the existing problems in the prior art, which may cause harm to the operator and pollute the environment under strong alkali conditions, or cause great losses and difficult recovery of tea saponin due to adsorption, and provide An environmentally friendly tea saponin recovery and low loss rate tea saponin enzyme decolorization process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Co-immobilization of laccase and glucose oxidase :Mix the 0.05 wt %% laccase aqueous solution and the 0.05 wt% glucose oxidase aqueous solution with equal volume, mix and stir with the 5 wt% sodium alginate solution in a volume ratio of 1:7, and then add the sodium alginate, etc. The volume of 5wt% gelatin solution is evenly mixed, adjust the pH to 4, after slow stirring for 80 minutes, the temperature of the mixture is cooled to 10℃, and then the mixture is injected into the 3% calcium chloride solution at a height of 7cm through the needle of the syringe The microspheres are formed in the microspheres, then keep the temperature at 4±1℃. After hardening for 8 hours, the microspheres are taken out and placed in a solution of 30℃, 5 wt% glutaraldehyde for further hardening for 1 hour, and then washed with saline to remove residual glutaraldehyde. , Get co-immobilized laccase and glucose oxidase;

[0027] (2) Preparation of crude tea saponin solution : Dissolve the crud...

Embodiment 2

[0032] (1) Co-immobilization of laccase and glucose oxidase :Mix 0.08 wt%% laccase aqueous solution and 0.08 wt% glucose oxidase aqueous solution with equal volume, mix and stir with 5 wt% sodium alginate solution in a volume ratio of 1:10, then add sodium alginate, etc. The volume of 5wt% gelatin solution is evenly mixed, the pH is adjusted to 4, and after slow stirring for 90 minutes, the temperature of the mixture is cooled to 8°C, and then the mixture is injected into the 5% calcium chloride solution at a height of 10cm through the needle of the syringe The microspheres are formed in the microspheres, and the temperature is maintained at 4°C. After hardening for 10 hours, the microspheres are taken out and placed in a solution of 30°C with a concentration of 5 wt% glutaraldehyde for further hardening for 2 hours, and then washed with saline to remove residual glutaraldehyde. Co-immobilized laccase and glucose oxidase;

[0033] (2) Preparation of crude tea saponin solution : ...

Embodiment 3

[0037] (1) Co-immobilization of laccase and glucose oxidase :Mix 0.1 wt%% laccase aqueous solution and 0.1 wt% glucose oxidase aqueous solution in equal volume, mix and stir with 6 wt% sodium alginate solution in a volume ratio of 1:9, and then add sodium alginate, etc. The volume of 6wt% gelatin solution is evenly mixed, adjust the pH to 5, after slow stirring for 100min, cool the mixture to 5℃, and then inject the mixture into the 5% calcium chloride solution at a height of 5cm through the needle of the syringe The microspheres are formed in the microspheres, then keep the temperature at 4℃, and after hardening for 8 hours, the microspheres are taken out and placed in a 30±2℃, 6 wt% glutaraldehyde solution for further hardening for 1 hour, and then washed with saline to remove residual glutaraldehyde , Get co-immobilized laccase and glucose oxidase;

[0038] (2) Preparation of crude tea saponin solution : Dissolve the crude tea saponin in a pH 5.5 acetic acid-sodium acetate bu...

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Abstract

The invention discloses a tea saponin bio-enzyme decoloring technology, and aims to solve the problems that: (1) in the prior art the decoloring process must be carried out in a strong alkaline condition, and the strong alkali will harm the operators and pollute the environment; (2) the loss of tea saponin is great and the recovery of the tea saponin is difficult because of the absorption effect. The technology comprises the following main steps: co-immobilization of laccase and glucose oxidase, preparation of a crude tea saponin solution, decoloring of crude tea saponin, and recovering of tea saponin. The technology has the advantages of mild reaction conditions, short decoloring time, and prominent decoloring effect. Moreover, the immobilized enzyme can be recycled and reutilized, the loss rate of tea saponin is low, the tea saponin powder with light color and luster can be recycled only through a spray drying technology, and the technology is simple.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a tea saponin biological enzyme decolorization process. Background technique [0002] Tea saponin is a pentacyclic triterpenoid glycoside compound extracted from the Theaceae plant. It is a nonionic surfactant with excellent performance and has strong emulsifying, dispersing, moisturizing, foaming, and foaming functions. It can be widely used in the fields of light industry, chemical industry, pesticide, feed, textile, mining and construction, etc., and can be used in the manufacture of emulsifiers, detergents, pesticide auxiliaries, feed additives, textile auxiliaries and coagulant inhibitors, etc. At present, the powder obtained by spray-drying the crude extract of tea saponin is used more, which contains a large amount of Maillard pigments, polyphenols and flavonoid pigment substances, and the aqueous solution of 5% crude extract of tea saponin is the color The deep tan color se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/06
Inventor 王莉
Owner HANGZHOU YIMIN PETOROCHEM
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