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Durably-transparent silk fibroin film and preparation method thereof

A silk fibroin, transparent technology, used in medical science, absorbent pads, prostheses, etc., can solve the problems of high strength, slow degradation, and low elongation of silk fibroin membranes, and achieve good light transmittance. The effect of biocompatibility and broad application prospects

Active Publication Date: 2014-06-18
PHARSUN MEDICAL BIOTECHNICS (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the Chinese invention patent "regenerated silk fibroin film and its preparation method" (CN101760027A), the insoluble silk film is prepared by adding ethanol and stretching. On the one hand, the addition of ethanol will affect the biocompatibility, on the other hand, the formation The β-sheet structure of silk fibroin film has high strength and low elongation, and the degradation rate is slow

Method used

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  • Durably-transparent silk fibroin film and preparation method thereof
  • Durably-transparent silk fibroin film and preparation method thereof
  • Durably-transparent silk fibroin film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation steps of the long-time transparent silk fibroin film provided in this embodiment are as follows:

[0023] 1. ① put 80g of raw silk into 4 liters of sodium carbonate aqueous solution with a mass concentration of 0.075%, treat it at 98-100°C for 30min, and wash it with deionized water. ②Then put silk fibroin in 0.05% aqueous sodium carbonate solution and treat it at 98-100°C for 30 minutes, take it out and wash it. ③Repeat ② once to get pure silk fibroin (ie silk fibroin) after fully washing. The dried pure silk fibroin was heated and dissolved at 60° C. with 1 liter of lithium bromide solution with a concentration of 9.0 mol / liter to obtain a silk fibroin mixed solution.

[0024] 2. Using cellulose membrane as the dialysis material, dialyze the obtained silk fibroin mixed solution with deionized water to remove impurities such as lithium bromide to obtain a pure silk fibroin solution. The mass concentration of silk fibroin is about 5 wt.%.

[0025] 3. A...

Embodiment 2

[0034]1. ① put 80g of raw silk into 4 liters of sodium carbonate aqueous solution with a mass concentration of 0.075%, treat it at 98-100°C for 30min, and wash it with deionized water. ②Then put silk fibroin in 0.05% aqueous sodium carbonate solution and treat it at 98-100°C for 30 minutes, take it out and wash it. ③Repeat ② once to get pure silk fibroin (ie silk fibroin) after fully washing. The dried pure silk fibroin was heated and dissolved at 60° C. with 1 liter of lithium bromide solution with a concentration of 9.0 mol / liter to obtain a silk fibroin mixed solution.

[0035] 2. Using cellulose membrane as the dialysis material, dialyze the obtained silk fibroin mixed solution with deionized water to remove impurities such as lithium bromide to obtain a pure silk fibroin solution with a mass concentration of silk fibroin of about 5%.

[0036] 3. Add formamide with a molecular weight of less than 150 to the pure silk fibroin solution, and the mass ratio of formamide to si...

Embodiment 3

[0044] 1. Prepare a 5wt.% silk fibroin solution according to the method described in Example 1, mix acetamide with a molecular weight of less than 150 with silk, and the mass ratio of the amount of acetamide added to silk fibroin is 20:80.

[0045] 2. Cut the above silk fibroin film sample into powder for testing. Using a fully automatic X’PERT PRO MPD ray diffractometer, test conditions: CuKα rays, tube voltage 40Kv, tube current 35mA, scanning speed 10 (°) / min, see the results in the attached figure 1 .

[0046] 3. Take the silk fibroin film, and press out the sample according to the GB / T1040-2006 standard I-shaped 3 cutter. The tensile mechanical properties were measured using an Instrong 3365 universal material testing instrument with a clamp distance of 28 mm and a tensile speed of 20 mm / min. Test environment: temperature 25 ℃, RH 65%, each group of samples was repeated 6 times, and the average value was taken. The main performance indicators of the transparent ...

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Abstract

The invention relates to a durably-transparent silk fibroin film and a preparation method thereof. The preparation method comprises the steps of: by taking silk fibroin as a raw material, adding amide with the molecular weight of less than 150 into fibroin solution to serve as a transparent crosslinking agent, and pouring into a film. The transmittance of the fibroin film in the visible light range of 400-800nm is more than 90%, and has durability. The prepared silk fibroin film is easily degradable and good in biocompatibility, and can be used as a cell carrier material on which cells normally grow. The material can maintain stable transparency for a long time, and thus has certain advantages as a cornea carrier or cornea substitution material, and can be used for contact lenses, artificial cornea, cell culture, wound repair materials and temporary coverage, tissue isolation and drug controlled release materials, and the like.

Description

technical field [0001] The present invention relates to a silk fibroin film and a preparation method thereof, in particular to a technique for preparing a silk fibroin film that remains transparent for a long time using silk fibroin as a raw material. The prepared material can be applied to artificial cornea and corneal tissue Engineering materials and wound covering materials and other fields. Background technique [0002] The cornea is the most transparent part of the eye that covers the iris, pupil, and anterior chamber and provides most of the eye's optical power. Corneal ulcers, injuries, and scars caused by various reasons are the main causes of corneal blindness. According to the United Nations World Health Organization report, corneal disease is the second most common blindness after cataract, and the number of cases is increasing at a rate of 1.5 to 2 million cases per year. Corneal transplantation is currently the only effective way to treat corneal blindness. Wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/50A61L15/32
Inventor 张珊珊卢神州李姣姣李翔胡柯豪解传芳
Owner PHARSUN MEDICAL BIOTECHNICS (SHANGHAI) CO LTD
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