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Penthorum chinense Pursh quality detection method

A detection method, the technology of driving yellow grass, which is applied in the field of quality detection of yellow grass, can solve the problems of difficult-to-catch yellow grass medicinal materials and their preparation evaluation and effective quality control, and achieve easy quality control, high separation, and exclusive strong effect

Inactive Publication Date: 2014-06-04
LUZHOU MEDICAL COLLEGE
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  • Description
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Problems solved by technology

However, for many years, there has been no revision of "Hunan Province Traditional Chinese Medicinal Materials Standards" to revise the item of "Huanhuangcao", and the standard mainly uses the content of quercetin as the standard, so it is difficult to conduct a comprehensive evaluation and effective quality control of the medicinal materials and their preparations.

Method used

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  • Penthorum chinense Pursh quality detection method
  • Penthorum chinense Pursh quality detection method
  • Penthorum chinense Pursh quality detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The content of the total flavonoids in the yellow grass of embodiment 1UV method determination

[0032] 1 Preparation of solution

[0033] Preparation of rutin reference substance solution Accurately weigh 10.6mg of rutin in a 10mL beaker, dissolve it with 50% ethanol, and set the volume in a 100mL volumetric flask, the concentration is 0.106mg·mL -1 .

[0034] Preparation of the test solution Precisely weigh about 0.1g of the fine powder into a 50mL conical flask with a stopper, add 10mL of 70% ethanol solution to each of the 5 conical flasks at a ratio of 100:1, shake well, seal, and avoid Stand in the light for 1h, ultrasonically extract in an ultrasonic cleaner for 1h, shake well, let stand to cool, and take the subsequent filtrate.

[0035] 2 Selection of measurement method and measurement wavelength

[0036] Take 0.2mL each of the rutin reference solution and the test solution in a 5mL volumetric flask, add water to the mark, shake well, add 0.1mol·mL -1 Alumi...

Embodiment 2

[0063] Embodiment 2 The selection of the method for measuring the color development of total flavonoids by ultraviolet spectrophotometry of the present invention

[0064] Flavonoids (Flavonoids) are based on C 6 -C 3 –C 6 The derivatives of chromane or chromone, whose structure is the basic core, are favored for their wide range of pharmacological effects. The content determination of total flavonoids in Chinese medicinal materials has become the focus of research on flavonoids in recent years. The colorimetric method used in the determination of total flavonoids in traditional Chinese medicine is the most common method for the determination of total flavonoids adopted by the "Chinese Pharmacopoeia". Among them, NaNO 2 -Al(NO 3 ) 3 -NaOH colorimetry is commonly used. This method uses NaNO 2 -Al(NO 3 ) 3 It is a chromogenic agent, and under alkaline conditions, it is characterized by the formation of red aluminum chelates with flavonoids. Taking rutin as a control, the...

Embodiment 3

[0067] The TLC identification of embodiment 3 gallic acid

[0068] Take 2.0g of Herba japonica fine powder, add 100mL of water, ultrasonically extract for 40min, filter, evaporate the filtrate to dryness, add 10mL of ethyl acetate to dissolve the residue, filter, and use it as the test solution. Take another gallic acid reference substance, add ethyl acetate to make a reference solution containing 0.5mg per 1mL. Take 10 μL of each of the above-mentioned test sample and reference solution, spot on the same silica gel G thin-layer plate, use chloroform-ethyl acetate-formic acid (6:4:1) as the developer, pre-saturate for 10 minutes, and develop upward. Take it out, dry it in the air, spray it with 5% ferric chloride ethanol solution, and bake it at 120°C for about 3 minutes. In the chromatogram of the test product, at the position corresponding to the chromatogram of the reference product, the same purple-black spots appear. see results image 3 .

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Abstract

The invention provides a content detection method of Penthorum chinense Pursh or extract. The detection method realizes simultaneous detection of general flavones and gallic acid in Penthorum chinense Pursh, effectively controls quality of medicinal material Penthorum chinense Pursh and its extract, has strong specificity and a high separation degree and can be used for controlling quality of products obtained in a laboratory and industrial large-scale production.

Description

technical field [0001] The invention relates to a method for detecting the quality of yellow grass. Background technique [0002] C. chinense Pursh is the dry aerial part of Penthorum chinense Pursh, a plant of the saxifrage family C. chinense Pursh. It mainly contains flavonoids, volatile oil and other ingredients. Among them, gallic acid and quercetin have been identified as the ingredients known to have anti-hepatitis B virus and liver protection effects, and are the active ingredients of the yellow grass. According to the existing literature, there are only research reports on its chemical components, and clinical reports on the use of Gansu Granules, a Chinese patent medicine containing this medicinal material, for the treatment of hepatitis and anti-hepatic fibrosis. However, for many years, there has been no amendment to the item of "Hunan Province Traditional Chinese Medicinal Materials" and the standard mainly uses the content of quercetin as the standard, so it i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N30/90G01N30/02
Inventor 税丕先何颖朱烨余昕李婷李建卢娟张春庄元春
Owner LUZHOU MEDICAL COLLEGE
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