Quality detection method for liver protection dropping pill of traditional Chinese medicine preparation

A quality inspection method and technology for protecting liver dripping pills, applied in the biological field to achieve the effect of quality control

Active Publication Date: 2014-05-21
HEILONGJIANG KUIHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Yet there is not yet an effective quality detection method for Hugan dripping pills in the prior art

Method used

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  • Quality detection method for liver protection dropping pill of traditional Chinese medicine preparation
  • Quality detection method for liver protection dropping pill of traditional Chinese medicine preparation
  • Quality detection method for liver protection dropping pill of traditional Chinese medicine preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The mensuration of active ingredient content in Hugan dripping pill of the present invention

[0052] (1) Detection of components of Bupleuri

[0053] Chromatographic conditions and system suitability test use octadecyl bonded silica gel as filler; acetonitrile as mobile phase A, water as mobile phase B; during the detection process, during 0-50 minutes, mobile phase A:B with ( 25→90:75→10) for gradient elution, during 50-55 minutes, use mobile phase A:B (90:10) for elution; the detection wavelength is 210nm; the number of theoretical plates is based on the peak of saikosaponin a The calculation is not less than 10000;

[0054] Preparation of reference substance solution Take an appropriate amount of saikosaponin a reference substance, weigh it accurately, add methanol to make a solution containing 0.4 mg of saikosaponin a per 1 ml, shake well, and obtain.

[0055] The preparation of need testing solution takes 100 balls of liver-protecting dripping pills, accurately ...

Embodiment 2

[0068] The identification method of embodiment 2 liver-protecting dripping pills of the present invention

[0069] (1) Identification of Schisandra

[0070] Take 40 pills of Hugan Dropping Pills, add 20ml of methanol, sonicate for 20 minutes, freeze for 2 hours, filter, put the filtrate on a water bath and evaporate to dryness, add 30ml of cyclohexane to the residue, sonicate for 30 minutes, filter, and use the medicine residue for later use; Put it on a water bath and evaporate to dryness, add 2ml of ethyl acetate to the residue to dissolve it, and use it as the test solution. Another Schizandrin B reference substance, add methanol to make a solution containing 1mg per 1ml, as the reference solution. According to the thin-layer chromatography test, draw 2 μl of each of the above two solutions, and spot them on the same silica gel GF respectively. 254 Use toluene-ethyl acetate (9:1) as the developer on the TLC plate, develop it, take it out, dry it, and inspect it under a UV...

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Abstract

The invention relates to a quality detection method for a liver protection dropping pill of traditional Chinese medicine preparation. The quality detection method comprises the following steps: measuring the content of effective components of the liver protection dropping pill, including saikoside a and schizandrin, and identifying schisandra chinensis, pulvis fellis suis and artemisia capillaris in the liver protection dropping pill. The quality detection method also comprises the following detection steps: (1) with the saikoside a as a reference substance, measuring whether a radix bupleuri component is contained in a liver protection dropping pill recipe by adopting a high efficiency liquid chromatography method; (2) with the schizandrin as the reference substance, measuring whether a schizandrin component is contained in the liver protection dropping pill by adopting the high efficiency liquid chromatography method; (3) with the schisandrin b as the reference substance, identifying whether a schisandra chinensis component is contained in the liver protection dropping pill by adopting a thin-layer chromatography method; (4) with hyodeoxycholic acid as the reference substance, identifying whether a pulvis fellis suis component is contained in the liver protection dropping pill by adopting the thin-layer chromatography method; and (5) with chlorogenic acid as the reference substance, identifying whether an artemisia capillaris component is contained in the liver protection dropping pill by adopting the thin-layer chromatography method. The quality detection method disclosed by the invention can be used for effectively and reliably controlling the quality of the liver protection dropping pill, and the method is scientific, feasible and reliable.

Description

technical field [0001] The invention relates to a quality detection method, in particular to a quality detection method of traditional Chinese medicine preparation Hugan dripping pills, and belongs to the field of biotechnology. Background technique [0002] Hugan dripping pills are six Chinese herbal medicines of Bupleurum, capillary, isatidis, schisandra, pig gall powder, and mung bean. , Polyethylene Glycol are mixed uniformly and are prepared into drop pills, and every pill weighs 40mg, and the appearance of this medicine is the drop pill of brown black to black; Slight gas, bitter in the mouth. The preparation is characterized by short dissolution time and high bioavailability. After the active ingredients in the medicinal materials are extracted by appropriate methods and purified to remove impurities, the dosage for each administration is reduced, which is convenient for patients to take. [0003] Hugan tablet is a new drug independently developed by our company in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/90
Inventor 关彦玲曲文华
Owner HEILONGJIANG KUIHUA PHARMA
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