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Preparation method of cartilage regeneration scaffold material

A scaffold material and cartilage technology, applied in the field of preparation of cartilage regeneration scaffold materials, can solve the problems of lack of immunogenicity and virus inactivation, and achieve the promotion of attachment and growth, good tissue affinity and compatibility, and repair of joints. defect effect

Active Publication Date: 2014-05-14
北京科健生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The scaffold material or carrier material of the second-generation technology has a certain activity or induction function. This scaffold can compound bone marrow stromal stem cells, and can make the composite bone marrow stromal stem cells differentiate into chondrocytes. At the same time, this scaffold material can also induce cells from other sources. Form cartilage; this tissue-engineered cartilage has a stronger ability to form cartilage than the first-generation tissue-engineered cartilage, but the surgery still uses an implantation method that does not destroy the subchondral bone
ZL201110099363.1 discloses a preparation method of a scaffold material for cartilage repair. Due to the lack of cryogenic treatment to reduce immunogenicity and virus inactivation process, there are potential biosafety risks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 Novel cartilage regeneration scaffold material and preparation method thereof

[0038] The whole layer of facial skin of calves that died shortly after birth was taken out, placed in a -80°C refrigerator, and frozen for 30 days. Then take it out, and after rewarming, depilate.

[0039] The depilatory process uses freshly prepared 8% Na 2 S solution, treated for 2h.

[0040] The virus was inactivated with peracetic acid-ethanol solution, the mass percentage of peracetic acid was 0.05%, the mass percentage of ethanol was 0.15%, the virus inactivation time was 2h, and the temperature was 24°C.

[0041] After virus inactivation, wash with normal saline for 8 times, each time for 20 minutes. After washing, check the pH value of the cleaning solution. When the pH reaches 6.5-7.5, it is the end point of washing.

[0042] Decellularization process: Soak the material with a concentration of 0.02% sodium dodecyl sulfate solution for 4 hours. The decellularization...

Embodiment 2

[0049] Embodiment 2 Novel cartilage regeneration scaffold material and preparation method thereof

[0050] The whole layer of skin on the back of suckling pigs that died shortly after birth was taken out, placed in liquid nitrogen at -196°C, and frozen for 4 days. Then take it out, and after rewarming, depilate.

[0051] The hair removal process uses a freshly prepared trypsin solution with a concentration of 0.5% for 18 hours.

[0052] Inactivate the virus with 0.15% ethanol solution for 4 hours at a temperature of 24°C.

[0053] After virus inactivation, wash with purified aqueous solution for 5 times, each time for 15 minutes. After washing, check the pH value of the cleaning solution. When the pH reaches 6.5-7.5, it is the end point of washing.

[0054] Decellularization process: Soak the material with 0.1% polyethylene glycol octyl phenyl ether solution for 6 hours. The decellularization process is carried out in an oscillating constant temperature ultrasonic cleaner i...

Embodiment 3

[0061] Example 3 Novel cartilage regeneration scaffold material and preparation method thereof

[0062] The whole layer of buttock skin of the pony that died shortly after birth was taken out and frozen at -20°C for 60 days. Then take it out, after rewarming, depilate with 7% thioglycolic acid for 2 hours, and then wash it with purified aqueous solution 5 times, 10 minutes each time.

[0063] Use tributyl phosphate plus surfactant Tween 80 for virus inactivation, the mass percentage content of tributyl phosphate in the virus inactivation solution is 0.3%, the mass percentage content of Tween 80 is 1%, and the treatment temperature is 24°C , the inactivation time is 6 hours.

[0064] After virus inactivation, wash with purified aqueous solution for 5 times, each time for 15 minutes. After washing, check the pH value of the cleaning solution. When the pH reaches 6.5-7.5, it is the end point of washing.

[0065] Decellularization process: Soak the material with 0.3% trypsin for...

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Abstract

The invention provides a preparation method of a cartilage regeneration scaffold material. The preparation method is characterized in that fresh skin at back, face or buttock of a dead animal is taken as a raw material and is subjected to treatment processes such as deep and low-temperature treatment, depilation, inactivation of virus, decellularization, structure remodeling, crosslinkage, freeze-drying, re-modification and sterilization, thus obtaining a finished product. The cartilage regeneration scaffold material prepared by the method disclosed by the invention can be used for promoting adhesion and growth of cartilage cells and promoting healing of articular cartilage injuries. The product belongs to a natural extracellular matrix ingredient which is beneficial to adsorption, proliferation and differentiation of the cells, also has a certain mechanical strength, can exist in the body for a long time in the form of a tissue filler, also has good tissue affinity and compatibility, and can be used for inducing cartilage cells to grow therein so as to rebuild the cartilage, promote the adhesion and growth of the cartilage cells, promote the healing of the articular cartilage injuries and restore articular defects, so the cartilage regeneration scaffold material is a novel regeneration scaffold material for effectively restoring articular cartilage defects.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for preparing a cartilage regeneration scaffold material. Background technique [0002] In the field of orthopedics, articular cartilage defects are very common diseases. The repair and treatment of articular cartilage injury has always been a difficult problem in the field of orthopedics. At present, artificial knee replacement is mostly used clinically to treat cartilage defects. Artificial joint replacement can easily lead to some complications, such as easy loosening, need for revision, high cost and excessive medical treatment. Therefore, further research and development of cartilage scaffold repair materials that can achieve endogenous tissue regeneration, improve domestic medical level and related treatment effects, and reduce domestic medical expenses have become an important direction in the field of clinical cartilage regeneration scaffold material...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/54C08J3/24
Inventor 陈德夫田伟孙磊牛睿
Owner 北京科健生物技术有限公司
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