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Isaria fumosorosea and beta-cypermethrin oil suspension and applications thereof

A technology of Corynebacterium rosacea and high-efficiency cypermethrin, applied in the application, insecticide, biocide and other directions, can solve the problems of low content of active ingredients, unstable control effect, difficulty in transportation and storage, etc., and achieve a low lethal concentration , alleviate the problem of drug resistance and improve the effect of insecticidal effect

Inactive Publication Date: 2014-04-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its active ingredient content is low and its volume is large, which brings great difficulties to transportation and storage. In addition, the amount of bacterial powder used is large, the adhesion is not good, and the control effect is very unstable.

Method used

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  • Isaria fumosorosea and beta-cypermethrin oil suspension and applications thereof
  • Isaria fumosorosea and beta-cypermethrin oil suspension and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 The preparation of rose fume-colored I. spp. conidia powder

[0044] The I. fumigatus used in the examples of the present invention is I. fumigatus strain SCAU-IFCF02, which was deposited in the China Center for Type Culture Collection on November 1, 2013, with a preservation number of CCTCC NO: M 2013526.

[0045] The preparation steps of the rose fume-colored clavula conidia powder of the present invention are as follows:

[0046] S1. First-level solid culture, take 50uL of the original bacterial liquid of I. fumigatus strain SCAU-IFCF02 and spread it on the SDAY solid medium (SDAY medium is the most suitable medium), and cultivate it at 24°C, the best culture The time is 15 days;

[0047] S2. Secondary liquid shaking culture, wash the spores on the SDAY solid medium with sterile water containing 0.03% Tween-80, and then filter with gauze to make 1×10 7 1 spore / mL of spore suspension, 100uL of the above spore suspension was inoculated into 200mL of Sabou...

Embodiment 2

[0054] Embodiment 2 prepares 15.8% rose fume-colored I. spp. and beta-cypermethrin oil suspension

[0055] 1. Preparation method:

[0056] S1. According to the formula requirements, add I. fumospora conidia powder, vegetable oil, and emulsifier into the mixing kettle. In a high-shear emulsifier machine, stir at 4000r / min for 10-15min to fully mix the materials evenly;

[0057] S2. Add the mixed product of S1 to beta-cypermethrin, stabilizer, suspending agent, dispersant, mineral oil or methyl oleate, continue high-shear emulsification in the mixing kettle, and stir at 4000r / min for 10-15min ;

[0058] S3. Put the product obtained in S2 into a sand mill for sufficient sand grinding to obtain a rose-colored fume-colored I. spp. and beta-cypermethrin oil suspension concentrate, which are measured and packaged to obtain the finished product.

[0059] 2. The 15.8% I. fumigatus and beta-cypermethrin oil suspension concentrate prepared in the present embodiment comprises the foll...

Embodiment 3

[0060] Example 3 Preparation of 15% rose fume-colored I. spp. and beta-cypermethrin oil suspension

[0061] 1, preparation method: with embodiment 2.

[0062] 2. The 15% I. fumigatus and beta-cypermethrin oil suspension concentrate prepared in the present embodiment comprise the following components in weight percent: I. fumigatus powder 14.6%, 0.4% beta-cypermethrin, soybean oil 18%, Alkylphenol polyoxyethylene ether 10%, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate 5%, epoxy linseed oil 3%, organic bentonite 1%, sodium alginate 2%, methyl oleate to 100% %.

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Abstract

The invention discloses an isaria fumosorosea and beta-cypermethrin oil suspension and applications thereof. The isaria fumosorosea and beta-cypermethrin oil suspension comprises the following ingredients in percentage by mass: 8-30% of isaria fumosorosea and beta-cypermethrin, 1-20% of plant oil, 10-20% of emulsifier, 4-10% of dispersing agent, 2-7% of stabilizer, 2-7% of suspending agent, and the balance of mineral oil or methyl oleate. The oil suspension is prepared by taking isaria fumosorosea and beta-cypermethrin as active ingredients, and is mainly used for preventing pests of coptotermes formosanus, plutella xylostella and the like, the complex insecticide has excellent compatibility, the insecticide toxicity of single agents can be obviously enhanced, the complex insecticide can kill various pests, and has the advantages of being synergistic, good in permeability, strong in adhesion, evaporation-resistant, environment-friendly, long in lasting effect period and the like.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides. More specifically, it relates to an oil suspension of I. fumigatus and beta-cypermethrin and its application. Background technique [0002] At present, in the prevention and control of agricultural pests in my country, the use of chemical pesticides to control pests and diseases is still one of the main means to increase crop yields. Extensive use of chemical pesticides seriously pollutes the environment on which human beings depend, destroys the ecological balance, and poses a serious threat to human health. Therefore, biological control is more and more concerned and valued by the world. As an important part of pest biological control, entomogenic fungi play an irreplaceable role in continuously controlling pests and protecting ecological balance. Rose smoke-colored Isospora ( Isaria fumosorosea ) is an important entomogenic fungus, widely distributed all over the world, with ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/04A01P7/04A01N53/08
Inventor 何余容念晓歌吕利华谢梅琼
Owner SOUTH CHINA AGRI UNIV
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