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Preparation method of absorbent for absorbing and degrading water pollutants

A water pollutant, adsorption and degradation technology, applied in the direction of adsorption water/sewage treatment, chemical instruments and methods, other chemical processes, etc., can solve the problems of long cycle of bioremediation technology

Active Publication Date: 2014-04-09
海南椰国热带水果食品加工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cycle of bioremediation technology is long, and at the same time, it is limited by whether there are efficient petroleum hydrocarbon degrading bacteria. The physical adsorption method faces the problem of reprocessing the adsorbent after the adsorption is saturated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Activate the strain Acetobacter xylinus for fermentation production of biological cellulose, and prepare seed liquid for fermentation production: the seed liquid medium contains mannitol 25g / l, yeast extract 5g / l, peptone 3g / l, each 1 Put 500ml of liquid culture medium in a 1-liter shaker flask, and cultivate it for 24 hours at 180 rotations and 30 degrees to obtain a seed solution;

[0024] (2) Add the seed liquid to the fermentation medium with an inoculum volume of 10% by volume. The fermentation medium contains: acetic acid: 0.2%, sucrose: 10%, and the rest is coconut water; then add it to the fermentation medium Petroleum-degrading bacteria, which are mixed bacteria of Pseudomonas 2, Arthrobacter petroleum phage 2 and Phanerochaete chrysosporium 3, the effective number of bacteria in the fermentation medium after inoculation is 10 8 cfu / mL;

[0025] (3) 30°C, culture in a 250ml culture dish for 14 days;

[0026] (4) Collect the bacterial film formed on the s...

Embodiment 2

[0030] (1) Activate the strain Acetobacter xylinus for fermentation production of biological cellulose, and prepare seed liquid for fermentation production: the seed liquid medium contains mannitol 25g / l, yeast extract 5g / l, peptone 3g / l, each 1 Put 500ml of liquid culture medium in a 1-liter shaker flask, and cultivate it for 24 hours at 180 rotations and 30 degrees to obtain a seed solution;

[0031] (2) Add the seed liquid to the fermentation medium with an inoculum volume of 8% by volume. The fermentation medium contains acetic acid: 0.2%, glucose: 10%, and the rest is coconut water; then add oil to the fermentation medium The degrading bacteria are the mixed bacteria of Corynebacterium 1, Bacillus methane-extremophilus 3, Umbrella bursa 1, Boletus pine cone 1, and silk film 1, and the effective number of bacteria in the fermentation medium after inoculation is 10 6 cfu / mL; then add 0.01% octylphenol ethoxylate to the fermentation medium;

[0032] (3) 30°C, culture in a 2...

Embodiment 3

[0037] (1) Activate the strain Acetobacter xylinus for fermentation production of biological cellulose, and prepare seed liquid for fermentation production: the seed liquid medium contains mannitol 25g / l, yeast extract 5g / l, peptone 3g / l, each 1 Put 500ml of liquid culture medium in a 1-liter shaker flask, and cultivate it for 24 hours at 180 rotations and 30 degrees to obtain a seed solution;

[0038](2) Add the seed liquid to the fermentation medium with an inoculation amount of 12% by volume, and the fermentation medium contains acetic acid: 0.2%, sucrose: 5%, fructose: 5%, and the rest is coconut water; Petroleum-degrading bacteria were inserted into the base, including Pseudomonas 1, Corynebacterium 1, Bacillus methanosaurus 1, Arthrobacter petroleum-phagia 1, Nocardia 1, Umberella bursa 1, Lactobacillus 1, The mixed bacteria of pine cone boletus 1, silk film fungus 1, silver hamster thorn sporum 1, Phanerochaete chrysosporium 1, the effective number of bacteria in the fe...

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Abstract

The invention relates to a preparation method of an absorbent for absorbing and degrading seawater pollutants. The preparation method comprises the following steps: (1) activating a strain for fermentation production of biological cellulose so as to prepare a seed broth for fermentation production; (2) adding the seed broth into a fermentation culture medium with the inoculation scale of 8%-12% in volume, and inoculating the fermentation culture medium with a petroleum degrading strain; (3) fermenting at 25-35 DEG C in a standing state for 8-20 days; and (4) collecting a biological cellulose film generated on the surface of the culture medium, washing the biological cellulose film by an alkali liquor, and drying the washed film until the water content of the film is lower than 50%. The absorbent obtained by the preparation method can fix the petroleum degrading strain in the three-dimensional structure of the biological cellulose to form the film structure, the film structure can be directly laid on the water surface, and thus the contact area of the absorbent and the water surface is increased; simultaneously, the property of the biological cellulose is stable, the petroleum degrading strain in the biological cellulose can be slowly and efficiently released to absorb and degrade the petroleum pollutant in water, so that the absorbent has the features of immobilization, high efficiency, long-term effectiveness and the like.

Description

technical field [0001] The invention relates to a method for degrading water body pollutants, in particular to a method for preparing an adsorbent for adsorbing and degrading water body pollutants. [0002] Background technique [0003] In recent years, with the increasing demand for oil and its products in various countries around the world, oil spill accidents have occurred frequently in the process of offshore mining, loading and unloading, transportation and utilization of oil, which not only caused a large amount of crude oil loss, but also caused serious pollution to the environment. It is increasingly threatening the ecological environment of water bodies and land areas, and has long-term harm to water body environment, natural resources and aquaculture resources. Therefore, oil spill pollution in water bodies has become a major environmental problem that people must face. [0004] At present, there are mainly physical methods, chemical methods and biological methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30C02F1/28
Inventor 钟春燕钟宇光
Owner 海南椰国热带水果食品加工有限公司
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