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Tissue culture method for rapidly propagating wild lilies

A tissue culture, rapid technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as weak growth potential, achieve high differentiation rate, strong root system, and improve induction efficiency and proliferation coefficient.

Active Publication Date: 2014-04-09
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the highest proliferation rate of adventitious buds is only 4.8%, and the growth vigor is relatively weak

Method used

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  • Tissue culture method for rapidly propagating wild lilies
  • Tissue culture method for rapidly propagating wild lilies
  • Tissue culture method for rapidly propagating wild lilies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) The young flower stalks of Lilium sulphureum Baker collected from Yunnan were placed in 500 times liquid containing 50% carbendazim, 50mg.L -1 Agricultural streptomycin and 50mg.L -1 In the aqueous solution of agricultural penicillin, shake at 150rpm for 3h for pre-disinfection treatment;

[0041] 2) Soak and shake the pretreated pedicels in 75% alcohol for 20 seconds, then soak and shake them in 10% sodium hypochlorite solution for 13 minutes, rinse with sterile water for 3 times, each time for 1 minute;

[0042] 3) Cut the young pedicel, cut it in half longitudinally, and cut it into about one centimeter, place the longitudinal section of the pedicel on the induction medium (MS+6-BA 0.5mg.L -1 +NAA 1.5mg.L -1 +Sucrose 30.0g.L -1 +MES 2g.L -1 +Agar 6.0g.L -1 , pH=5.8~6.0) for induction culture, culture condition is temperature (25±2)℃, shading; such as figure 1 , figure 2 shown

[0043] 4) After 5 days, adventitious buds were induced, and the induced advent...

Embodiment 2

[0049] 1) Put the young pedicels of Lilium leucanthum (Baker) Baker collected from Yichang, Hubei into a solution containing 500 times of 50% carbendazim, 50mg.L -1 Agricultural streptomycin and 50mg.L -1 In the aqueous solution of agricultural penicillin, shake at 150rpm for 4h for pre-disinfection treatment;

[0050] 2) Soak and shake the pretreated pedicels in 75% alcohol for 20 seconds, then soak and shake them in 10% sodium hypochlorite solution for 13 minutes, rinse with sterile water for 3 times, each time for 1 minute;

[0051] 3) Cut the young pedicel, cut it in half longitudinally, and cut it into about one centimeter, place the longitudinal section of the pedicel on the induction medium (MS+6-BA 0.5mg.L -1 +NAA 1.5mg.L -1 +Sucrose 30.0g.L -1 +MES 2g.L -1 +Agar 6.0g.L -1 , pH=5.8~6.0) for induction culture, culture condition is temperature (25±2)℃, shading; such as Figure 5 as shown,

[0052] 4) After 5 days, adventitious buds were induced, and the induced ad...

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Abstract

The invention relates to a tissue culture method for rapidly propagating wild lilies and belongs to the field of a flower culture technology. According to the method, bud-stage flower stems of the lilies are used as explants of tissue culture of the lilies and are completely disinfected before culture; besides that 6-BA (Benayl Aminopurine), NAA (Naphthalene Acetic Acid) and IBA (Indole Butyric Acid) are used as auxins or hormones in a culture medium, an MES (Methyl Ester Sulfonate) is added to be used as a buffering agent so as to effectively control the pH value in a tissue culture process, and the pH value is maintained in the optimal range of differentiation, propagation and growth of the lily explants; therefore, the induction efficiency and the propagation coefficient are improved, the induction rate is 98%-100%, the quantity of adventitious buds induced by each explant is not less than 15, the propagation coefficient is 5-7, the rooting rate is 100%, and the root system is healthy and strong. The tissue culture method is applicable to safe propagation of good single plants of filial generations and is an effective manner for protecting wild resources of lilies.

Description

technical field [0001] The invention relates to a tissue culture method suitable for the protection of wild lily resources and the rapid propagation of excellent hybrid offspring, and belongs to the technical field of flower culture. Background technique [0002] Lily is the general term for plants of the genus Lilium in the family Liliaceae. Its flowers are huge and colorful, and have high ornamental, edible and medicinal values. There are about 100 species and variants of lily wild resources in the world, of which about 55 species and variants are native to China, with nearly 10,000 species. The collection and protection of wild lily resources is an important basis for lily breeding. Due to human and natural influences, on the one hand, the original habitat of natural resources has been severely damaged, and many species are facing the danger of extinction. The breeding and protection of wild lily germplasm resources is imminent. On the other hand, modern society has a st...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 明军袁素霞刘春徐雷锋
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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