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Application of ciclopirox olamine in preparing medicine for treating or preventing influenza virus infection

A ciclopirox amine and influenza prevention technology, applied in the field of medicine, can solve the problems such as no reports that ciclopirox amine is anti-influenza virus, and achieve the effect of broad-spectrum anti-influenza virus activity

Inactive Publication Date: 2014-04-02
武汉威立得生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have shown that it can inhibit HIV-I gene expression, but so far there has been no report on the anti-influenza virus of ciclopirox olamine

Method used

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  • Application of ciclopirox olamine in preparing medicine for treating or preventing influenza virus infection
  • Application of ciclopirox olamine in preparing medicine for treating or preventing influenza virus infection
  • Application of ciclopirox olamine in preparing medicine for treating or preventing influenza virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Evaluation of the anti-influenza virus activity of ciclopirox olamine

[0032] 1. Experimental Materials

[0033] 1.1 Cells, viruses and drugs

[0034] MDCK cells were purchased from American Type Culture Collection (ATCC); virus strain: A / PuertoRico / 8 / 34 (H1N1); drug: ciclopirox was purchased from Sigma.

[0035] 1.2 Experimental Instruments

[0036] Multilabel microplate reader (Perkin Elmer).

[0037] 2. Experimental methods and results

[0038] 2.1 Cell culture: 37°C, 5% CO 2 cultured in a humidified incubator. A DMEM medium containing 10% FBS, 100 U / mL of penicillin and streptomycin was used. Cells were subcultured after reaching 90% confluence, and the subculture ratio was 1 / 3 – 1 / 4.

[0039] 2.2 Virus culture: Take SPF chicken embryos aged 9 to 11 days, check them with an egg tester before inoculating the virus, and mark them at a position far away from the embryos, disinfect and punch holes, and inoculate 2- 4 The virus liquid wi...

Embodiment 2

[0049] Example 2: Evaluation of the broad-spectrum anti-influenza virus effect of ciclopirox olamine

[0050] In this experiment, ciclopirox olamine was tested against influenza A subtype H1N1 strains, influenza A virus subtype H3N2 strains, influenza A virus subtype H1N1 amantadine-resistant strains, influenza A virus subtype H6N6 strains, and influenza A virus subtype H6N6 strains. Antiviral activity of influenza virus subtype H7N8 strain, influenza A virus subtype H9N2 strain and influenza B virus.

[0051] 1. The virus strains used in the experiment include: A / Human / Hubei / 1 / 2009(H1N1), A / human / Hubei / 3 / 2005 (H3N2), A / human / WSN / 33 / (H1N1,S31N), A / Duck / Hubei / 5 / 2010(H6N6), A / Duck / Hubei / 216 / 1983 (H7N8), A / Chicken / Jiangsu / 1 / 2005(H9N2), B / human / Hubei / 1 / 2007.

[0052] 2. Divide MDCK cells into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates, cultured in a 37°C cell culture incubator for 14-18 hours, and the cells were grown into a single layer before use. D...

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PUM

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Abstract

The invention discloses application of ciclopirox olamine in preparing a medicine for preventing or treating influenza virus infection. According to the application, firstly, the toxicity of ciclopirox olamine to MDCK (madin-darby canine kidney) cells is detected, and results indicate that maximum non-cytotoxic concentration of the ciclopirox olamine is 50.0 mu M; secondly, the anti-virus activity of the ciclopirox olamine is detected in a complete non-cytotoxic concentration range, and results indicate that the small molecular compound has a remarkable anti-virus activity, and the anti-virus effect shows a dose dependent effect; finally, the anti-virus activity of the ciclopirox olamine to different types and sub-type influenza viruses is detected, and results indicate that ciclopirox olamine can be used for inhibiting replication of all detected influenza virus strains in a dose dependent manner, and the anti-influenza virus activity of the ciclopirox olamine has broad spectrum. Therefore, the ciclopirox olamine is a novel broad-spectrum anti-influenza virus medicine, and can be used for preparing the medicine for preventing or treating influenza virus infection.

Description

technical field [0001] The invention relates to the application of ciclopirox olamine in the preparation of medicines for treating or preventing influenza virus infection, and belongs to the technical field of medicine. Background technique [0002] Influenza viruses belong to the family Orthomyxoviridae, the genus Influenzavirus. According to the differences in the antigenic and genetic characteristics of the virion nucleoprotein (NP) and matrix protein (M), influenza viruses are divided into three types: A, B, and C, also known as A, B, and C. The whole genome of influenza A virus consists of eight single-stranded negative-strand RNAs of different sizes, named after segment 1 to segment 8, respectively. The full-length viral genome is about 13.6 kb, encoding 10 structural proteins (PB2, PB1, PA, HA, NP, NA, M1, M2, PB1-F2 and NS2 / NEP) and non-structural proteins (NS1). Influenza A viruses can be further divided into 17 H (H1-H17) and 10 N (N1-N10) subtypes according to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4412A61P31/16
Inventor 陈绪林安利伟刘锐
Owner 武汉威立得生物医药有限公司
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