Allergen colloidal gold diagnostic test strip for allergic diseases and preparation method thereof

Abstract

The invention discloses an anaphylactic disease allergen colloidal gold diagnostic test strip. The test strip comprises a sample hole, a colloidal gold pad, a film strip, an absorbent pad and a PVC board, wherein the film strip is coated with inhalation group allergen I, inhalation group allergen II, a food group allergen and a mouse anti-goat IgG antibody, so as to form three detecting strips and one quality control strip. Each allergen energy included in the test strip can represent allergen molecules of 80 percent of patients, and on the detecting film strip, a symbolic allergen coated molecule replaces an allergen extract, so that the purpose of quickly diagnosing the anaphylactic disease technically is achieved. The time for detecting by using the test strip lasts for 5 to 30 minutes, the specificity is high, the sensitivity is high, the testing is simple and quick, on-site detecting is achieved, special training for operators is not required, operation can be finished according to specification, and quick diagnosis to anaphylactic diseases can be achieved.

Description

technical field [0001] The invention relates to the field of allergen detection, in particular to an allergic disease allergen colloid gold diagnostic test strip and a preparation method thereof. Background technique [0002] Allergic diseases (also known as allergic diseases) include atopic dermatitis, food allergy, allergic rhinitis and allergic asthma, etc., and their incidence is increasing day by day, and the condition is becoming more and more complicated. WHO has listed allergic diseases as key research and prevention diseases in the 21st century. In recent years, with the development of immunohistochemistry, molecular biology techniques and clinical new technologies, such as fiberoptic bronchoscopy, there has been a consensus that this disease belongs to allergic inflammation, and there are a large number of inflammatory cells (including eosinophils) in the inflammatory area. granulocytes, lymphocytes, mast cells, basophils, etc.) The pathogenesis of the disease ma...

AI Technical Summary

Problems solved by technology

[0011] Currently on the market, the allergen in vitro diagnostic test strips for allergic diseases are based on Western blot and enzyme-linked immunoassay. The operation time is long (150min-240min), and the operation steps are complicated (including incubation, elution, etc.) , the supporting equipment is expensive (100,000 to 300,000 yuan), and the operation requires professionally trained technicians to operate

Seriously affected the popularization of allergen in vitro detection methods. At present, only large domestic hospitals have the ability to detect allergens in vitro, while most grassroots hospitals and army field hospitals cannot carry out in vitro allergen detection methods.

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