Anaphylactic disease allergen colloidal gold diagnostic test strip and preparation method thereof
A technology for allergic diseases and test strips, applied in the field of allergen detection, can solve the problems of complex operation steps, affecting the popularization of in vitro detection methods for allergens, and expensive supporting equipment.
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Embodiment 1
[0079] Preparation of Example 1 Allergen Colloidal Gold Diagnostic Test Strip for Allergic Diseases
[0080] 1. Coating the allergen marker molecular mixture to form three bands on the membrane strip
[0081]Among them, the allergens in the inhalation group I include: House dust mite, Dermatophex farinae, A. tropicalis, Blattella germanica, cat dander, dog dander, Alternaria, Aspergillus fumigatus, and a mixture of allergen marker molecules of Penicillium pointis wherein the signature allergen molecules in the mixture are: Derp1, Derp2, Derf1, Derf2, Blot5, Blag1, Blag2, Canf1, Canf2, Feld1, Feld2, Alta1, Aspf1, Aspf2, Aspf3, Aspf4, Aspf5, Aspf6, Penl3.
[0082] Inhalation group allergen II, including: artemisia pollen, ragweed pollen, humulus pollen, corn pollen, quinoa pollen, sycamore pollen, birch pollen, poplar pollen, willow pollen, elm, tree pollen; mixtures thereof The iconic allergen molecules in the medium are: Artv1, Artv4, Amb1, Humj1, Humj2, Zeam12.3, Cheal, Plaa...
Embodiment 2
[0108] Example 2 Quality Inspection of Allergen Colloidal Gold Diagnostic Test Strips for Allergic Diseases
[0109] The quality standard of the test strip prepared in Example 1 of the present invention is based on the three parts of the "Pharmacopia" of the People's Republic of China, and the minimum detection limit, the compliance rate of negative reference products, the compliance rate of positive reference products, and precision all meet the national standards (Table 1). .
[0110] Table 1 Quality standard and inspection of rapid diagnostic test strips for allergic diseases
[0111]
Embodiment 3
[0112] Example 3 Stability investigation of allergic disease allergen colloidal gold diagnostic test strips
[0113] The test strip prepared in Example 1 of the present invention was placed at 37°C for 8 days, and then tested with standard serum, CV<15% (Table 2).
[0114] The stability test result of table 2 test strip
[0115]
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