Detection device for electrochemical immune biosensor and detection and analysis method

A biosensor and detection device technology, applied in the field of biosensors, can solve the problems of detection limit, sensitivity and repeat consistency reduction, background noise, interference, etc., and achieve the effect of speeding up and actual reaction process, improving detection efficiency and improving efficiency

Active Publication Date: 2015-07-15
浙江象立医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Secondly, even if the antibody / antigen that has crossed this energy barrier collides or binds within the effective reaction scale, there is still a certain probability of non-specific binding, which will bring background noise and interference, resulting in detection limits, sensitivity and Reduction in performance such as repeat consistency

Method used

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  • Detection device for electrochemical immune biosensor and detection and analysis method
  • Detection device for electrochemical immune biosensor and detection and analysis method
  • Detection device for electrochemical immune biosensor and detection and analysis method

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Example 1. If figure 1 , figure 2 , image 3 As shown, the device of the present invention includes an acoustic energy transmission and electrochemical immune reaction detection device part, and a control detection part.

[0053] The part of the electrochemical immunoreaction detection device includes, from bottom to top, a transducer fixed substrate 110, an acoustic energy damping and attenuating sheet 109, an ultrasonic transducer 101, an ultrasonic coupling colloid 102, an acoustic focusing lens array integrated glass substrate 103, and synaptic contacts. Silicone rubber sealing ring 108, replaceable electrochemical immunobiological sensitive film substrate 104, polymer gold film electrode substrate 107, multi-channel weak electric signal detection circuit 106;

[0054] The integrated glass substrate 103 of the acoustic focusing lens array, the contact silicon rubber sealing ring 108, and the polymer gold film electrode substrate 107 are sequentially arranged from...

Embodiment 2

[0067] Embodiment 2. An analysis method for electrochemical immunobiological reaction measurement, specifically a microfluidic injection electrochemical immunobiological detection analysis method that utilizes controllable ultrasonic radiation energy to improve the efficiency of electrochemical immune reaction.

[0068] Step (1). Adjust the radiation energy field of the ultrasonic transducer 101

[0069] The focal length is adjusted by the size of the concave spherical surface of the acoustic lens on the ultrasonic coupling colloid 102, so that the vertical distance from the focal point to the surface of the replaceable electrochemical immunobiological sensitive membrane substrate 104 is 1-2 mm.

[0070] The characteristic size of the concave spherical surface of the acoustic lens on the ultrasonic coupling colloid 102 is determined by the characteristics of the ultrasonic frequency, the focused energy, the material and thickness of the polymer gold film electrode substrate 107...

Embodiment 3

[0083] Example 3. The typical preparation process of the replaceable electrochemical immunobiologically sensitive membrane substrate 104: ① Dissolving the antigen to be tested in a buffer solution, the selected buffer solution varies with the type of antigen, the principle is to make the activity and chemical reaction of the immune reaction Luminescent signal response is maximized. ② Pre-treat the surface of the sensor carrier to obtain a flat, clean and hydrophilic surface. ③ Prepare a certain concentration of epoxypropylene trimethylsilane solution, let it stand for 60 minutes to fully hydrolyze, then pipette 50 microliters of the solution and drop it on the surface of the carrier, and heat it at about 90°C for 60 minutes. Drop 50 microliters of 1% chitosan-acetic acid solution on the glass slide treated with silanization of GPS, and then place it in an oven and heat it at 105°C for 60 minutes to obtain a silane-crosslinked glutaraldehyde-based film. ④ Drop 50 microliters o...

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Abstract

The invention discloses a detection device for an electrochemical immune biosensor and a detection and analysis method. The device comprises a sound energy transmission and electrochemical immune reaction detection device part and a control detection part. The electrochemical immune reaction detection device part comprises an ultrasonic transducer, an ultrasonic coupling colloid, a sound focusing lens array integrated glass base plate, a replaceable electrochemical immune bio-sensitive membrane substrate, a macromolecular gold membrane electrode base plate and a multi-channel weak electric signal detection circuit; the control detection part comprises an ultrasonic sensor, a PID (Proportion Integration Differentiation) ultrasonic energy mode control module, a weak electric signal processing module, a data analysis and feedback control module and an energy control mode and immune electrochemical reaction system database. The method comprises the following steps: adjusting a radiation energy field of the ultrasonic transducer; carrying out a micro-flow injection analysis; and carrying out intelligent measure and control on ultrasonic emission energy. According to the detection device for the electrochemical immune biosensor and the detection and analysis method, predominant detection low limit, sensitivity, accuracy, repeatability and stability are represented and a preparation method is developed and simple.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to an electrochemical immune biosensor detection device and a detection analysis method, in particular to an electrochemical immune sensor detection device based on the sonochemical principle and an analysis method for electrochemical immune biological reaction measurement. Background technique [0002] As an immunoassay method combining immunoassay method with electrochemical detection, the electrochemical immunoassay biosensor is easy to form due to its good selectivity, fast analysis speed, simple operation, high sensitivity of electrochemical analysis, and immunoassay. The high selectivity and specificity of the analysis, the characteristics of the test instrument with high performance and low price ratio, are being widely used in various fields such as medical health, food safety, and environmental monitoring. However, there are also some problems in its application process. F...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N33/53
Inventor 刘军
Owner 浙江象立医疗科技有限公司
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