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A kind of water locking damage treatment agent based on biosurfactant and preparation method thereof

A technology of water lock damage and biological surface, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as difficulty in large-scale promotion, achieve good compatibility, and prevent and relieve water lock damage.

Active Publication Date: 2016-09-28
大连知微生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention uses the fermented liquid of microbial strains producing rhamnolipids, lipopeptides, trehalolipids and sophorolipids as the main agent, supplemented by various chemical surfactants to prepare water-locking damage The treatment agent solves the problem that the existing water lock damage treatment agent does not have a relatively broad-spectrum effect, effectively removes water lock, and is difficult to promote in a large area

Method used

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  • A kind of water locking damage treatment agent based on biosurfactant and preparation method thereof
  • A kind of water locking damage treatment agent based on biosurfactant and preparation method thereof
  • A kind of water locking damage treatment agent based on biosurfactant and preparation method thereof

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Embodiment 1

[0027] A preparation method based on biosurfactant-based water lock damage treatment agent, said preparation method comprising the steps of:

[0028] ①At 35°C, stir 65% of the microbial fermentation broth, 2.5% of alkylphenol polyoxyethylene ether, and 0.5% of non-ionic fluorocarbon surfactant for 30 minutes;

[0029] ②Add 5% cetyltrimethylammonium bromide and 27% distilled water to the solution obtained in step ①, and react at 50°C for 1 hour.

[0030] The preparation method of above-mentioned microorganism fermented liquid is:

[0031] ① Inoculate Pseudomonas aeruginosa ATCC 9027 in solid medium and culture at 35°C for 24 hours;

[0032] ② Inoculate the single colony of the solid medium obtained in step ① into the seed medium, and culture with shaking at 35°C and 150rpm for 1 day.

[0033] ③Add the seed liquid obtained in step ② into the fermentation medium, incubate at 35°C for 48 hours, and filter to obtain a rhamnolipid microbial fermentation liquid with a surface tensi...

Embodiment 2

[0038] A preparation method based on biosurfactant-based water lock damage treatment agent, said preparation method comprising the steps of:

[0039] ①At 35°C, stir 50% by mass microbial fermentation broth, 3% alkylphenol polyoxyethylene ether, and 0.7% nonionic fluorocarbon surfactant for 30 minutes;

[0040] ②Add 5% cetyltrimethylammonium bromide and 41.3% distilled water to the solution obtained in step ①, and react at 50°C for 1 hour, and the surface tension is 30.0mN / m.

[0041] The preparation method of above-mentioned microorganism fermented liquid is:

[0042] ① Inoculate Bacillus subtilis ATCC 21332 in solid medium and culture at 37°C for 20h;

[0043] ② Inoculate a single colony on the solid medium obtained in step ① into the seed medium, and culture with shaking at 37°C and 170rpm for 24h.

[0044] ③ adding the seed liquid obtained in step ② into the fermentation medium, culturing at 37° C. for 48 hours, and filtering to obtain lipopeptide microbial fermentation l...

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Abstract

The invention relates to a water block damage treating agent based on a biological surface active agent and a preparation method thereof, and belongs to the technical field of oil field working fluid additives. The water block damage treating agent comprises the following components in percentage by weight: 30%-70% of microbe fermentation liquor, 1%-5% of non-ionic surface active agents, 0.5%-1% of fluorocarbon surface active agents, 5%-8% of cationic surface active agents and the balance of distilled water, wherein the microbe fermentation liquor is the microbe strain fermentation liquor of the biological surface active agent which generates one of rhamnolipid, lipopeptid, trehalose glycolipid and sophorolipid. The water block damage treating agent disclosed by the invention has the advantages of relatively broad-spectrum effect, high-efficiency water block release and easiness for large-area popularization.

Description

technical field [0001] The invention relates to a biosurfactant-based water lock damage treatment agent and a preparation method thereof, belonging to the technical field of oilfield well-entry fluid additives. Background technique [0002] In my country, the development potential of low-permeability oil and gas fields is huge. In 2008 alone, the production of low-permeability crude oil accounted for 37.6% of the total national crude oil production, and the production of low-permeability natural gas accounted for 42.1% of the total national natural gas production. At the same time, most of the current main production layers in the old oil regions in eastern my country are medium-high permeability reservoirs that are already in the high water-cut period, with a recovery rate of about 28%, a recovery rate of recoverable reserves of more than 70%, and a water cut generally as high as 90%. , the natural decline rate is more than 11%. If there is no replacement of new reserves, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K8/584C12N1/20C12P19/02C12P21/00C12R1/385C12R1/125
CPCC09K8/582C09K8/584C12P19/02C12P19/12C12P21/00
Inventor 闫海涛赵静付娜白剑李轩石明杰肖伟刘琳珊
Owner 大连知微生物科技有限公司
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