Natural bacteriostat composition applied to synergistic inhibition to common microbes

A composition and technology of antibacterial effect, applied in food science, food preservation, application, etc., can solve problems such as large dosage, hidden safety hazards, and narrow antibacterial spectrum

Inactive Publication Date: 2014-02-05
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The use of a single antibacterial agent often has the problem of narrow antibacterial spectrum and high dosage, which has potential safety hazards

Method used

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  • Natural bacteriostat composition applied to synergistic inhibition to common microbes
  • Natural bacteriostat composition applied to synergistic inhibition to common microbes
  • Natural bacteriostat composition applied to synergistic inhibition to common microbes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Nisin and ε-polylysine synergistically inhibit Escherichia coli

[0012] Take a 96-well polystyrene microwell plate, after ultraviolet disinfection, use a micropipette to add: 15.626, 7.813, 3.907, 1.953 to the wells of each column along the X-axis direction (from left to right) in the 1st to 8th columns in turn: , 0.977, 0.488, 0.244, 0.122 μg / mL ε-polylysine 25 μL, in the same way, add 125.00, 62.50, 31.25 , 15.625, 7.8125, 3.906, 1.953, 0.977 μg / mL nisin solution 25 μL, mix the two drugs evenly. Add 50 μL of the prepared Escherichia coli bacterial solution in turn, cover the upper plate after adding the solution, shake and mix slightly, place in a constant temperature incubator at 37°C for 24 hours and observe the results, repeat 3 times. Table 2 shows the results of the synergistic inhibition of Escherichia coli by nisin and ε-polylysine. Wells marked with * are synergistic antibacterial wells, and FIC is obtained by substituting into the formula I =0.2...

Embodiment 2

[0017] Example 2: Nisin and ε-polylysine synergistically inhibit Staphylococcus aureus

[0018] Take a 96-well polystyrene microwell plate, after ultraviolet disinfection, use a micropipette to add: 31.26, 15.63, 7.815, 3.908 to the wells of each column along the X-axis direction (from left to right) in the 1st to 8th columns , 1.954, 0.977, 0.488, 0.244 μg / mL ε-polylysine 25 μL, in the same way, add 250.00, 125.00, 62.50 , 31.25, 15.625, 7.813, 3.906, 1.953 μg / mL nisin solution 25 μL, mix the two drugs evenly. Add 50 μL of the prepared Staphylococcus aureus bacterial solution in turn, cover the upper plate after adding the solution, shake and mix slightly, place in a constant temperature incubator at 37°C for 24 hours and observe the results, repeat 3 times. Table 3 shows the results of synergistic inhibition of Staphylococcus aureus by nisin and ε-polylysine, and wells marked with * are synergistic antibacterial wells. Substitute into the formula to get FIC I =0.281. Tha...

Embodiment 3

[0023] Example 3: Nisin and ε-polylysine synergistically inhibit Bacillus subtilis

[0024] Take a 96-well polystyrene microwell plate, after ultraviolet disinfection, use a micropipette to add: 62.50, 31.25, 15.625, 7.812 to the wells of each column along the X-axis direction (from left to right) in the 1st to 8th columns , 3.906, 1.953, 0.977, 0.488 μg / mL ε-polylysine 25 μL, add 125.00, 62.50, 31.25 , 15.625, 7.8125, 3.906, 1.953, 0.977 μg / mL nisin solution 25 μL, mix the two drugs evenly. Add 50 μL of the prepared Bacillus subtilis bacterial solution in turn, cover the upper plate after adding the solution, shake and mix slightly, place in a constant temperature incubator at 37°C for 24 hours and observe the results, repeat 3 times. Table 4 shows the results of the synergistic inhibition of Bacillus subtilis by nisin and ε-polylysine. Wells marked with * are synergistic antibacterial wells, and FIC is obtained by substituting into the formula I =0.500. That is, the mass ...

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Abstract

The invention relates to a natural bacteriostat composition applied to synergistic inhibition to common microbes. The composition of epsilon-polylysine and nisin according to different proportions realizes the synergistic function on inhibition of escherichia coli, micrococcus luteus, bacillus subtilis, staphylococcus aureus and aspergillus niger, and is also applied to the freshness preservation of fresh-cut carrots. According to the natural bacteriostat composition applied to the synergistic inhibition to the common microbes, which is disclosed by the invention, the problem that the use amount of single bacteriostat is relatively large in the conventional bacteriostasis method is solved by the composition of the epsilon-polylysine and the nisin, the antibacterial spectrum is expanded by simultaneously utilizing the composition of the two bacteriostat, and the natural bacteriostat composition disclosed by the invention can be applied to food preservation under the condition of lower dosage and is high in safety.

Description

(1) Technical field [0001] The invention relates to a natural inhibitor composition synergistically inhibiting common microorganisms, which belongs to the category of food safety preservation. (2) Background technology [0002] With the improvement of people's living standards, the concept of green food and healthy food is more and more accepted by people. Long-term use of chemical preservatives will cause harm to people's health. People hope to use natural food preservatives to replace chemical preservatives. At present, the foreign food preservative industry is quite mature, and dozens of natural food preservatives have been developed. Nisin, also known as nisin (nisin), is a small molecular polypeptide substance produced by lactic acid streptococcus. It is a white solid powder and is relatively stable at room temperature and acidic conditions. It is the first safe bacteriostatin certified by the United States. At present, nisin has been approved by more than 60 countries...

Claims

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Application Information

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IPC IPC(8): A23L3/3463
CPCA23L3/3463
Inventor 李大鹏刘洪霞
Owner SHANDONG AGRICULTURAL UNIVERSITY
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