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Method for preparing high bio-availability blueberry anthocyanin

A blueberry anthocyanin, utilization technology, applied in the direction of organic chemistry, fermentation, etc., can solve the problems of blueberry anthocyanin activity reduction, lack of specificity in hydrolysis reaction, unsatisfactory application effect, etc., to achieve elimination of inhibition, high Health care value, the effect of shortening the production cycle

Active Publication Date: 2014-01-15
江苏沃田集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acid hydrolysis method requires high temperature, and the hydrolysis reaction lacks specificity. In addition to hydrolyzing glycosidic bonds, it can also destroy other groups on the mother nucleus, resulting in the reduction or even disappearance of blueberry anthocyanin activity, and acid hydrolysis will pollute the environment. The application effect is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A specific preparation operation steps of blueberry anthocyanins with high bioavailability are as follows:

[0026] (1) Preparation of blueberry anthocyanin solution; take commercially available blueberry anthocyanin extract, dissolve in acetic acid-sodium acetate buffer solution with a pH value of 5.0, and prepare 30g blueberry anthocyanin per 100 ml buffer solution blueberry anthocyanin solution of blueberry extract;

[0027] (2) Glycosidase-catalyzed conversion of blueberry anthocyanins; put the blueberry anthocyanin solution into the enzyme reactor, and add the activity of 500 U / mg according to the ratio of blueberry anthocyanin solution: glycosidase = 100 ml: 1g β-glucosidase, the temperature is 40 ° C, the stirring speed is 50 rpm, and the enzyme-catalyzed conversion reaction is carried out for 5 hours under the conditions of avoiding light to obtain the blueberry anthocyanin conversion liquid;

[0028] (3) Membrane separation of blueberry anthocyanin conversion ...

Embodiment 2

[0032] A specific preparation operation steps of blueberry anthocyanins with high bioavailability are as follows:

[0033] (1) Preparation of blueberry anthocyanin solution: take the blueberry anthocyanin extract and dissolve it in a citric acid-sodium citrate buffer solution with a pH value of 3.0, and prepare 50 g of blueberry anthocyanin extract per 100 ml of buffer solution blueberry anthocyanin solution;

[0034] (2) Glycosidase-catalyzed conversion of blueberry anthocyanins; put the blueberry anthocyanin solution into the enzyme reactor, add the activity according to the ratio of blueberry anthocyanin solution: glycosidase = 100 ml: 5g, respectively, 100 U / mg and 150 U / mg of α-arabinosidase and β-galactosidase, at a temperature of 35 ° C, a stirring speed of 80 rpm, and dark conditions, the enzyme-catalyzed conversion reaction was carried out for 3 hours to obtain blueberry anthocyanins prime conversion solution;

[0035] (3) Membrane separation of blueberry anthocyani...

Embodiment 3

[0039] A specific preparation operation steps of blueberry anthocyanins with high bioavailability are as follows:

[0040] (1) Preparation of blueberry anthocyanin solution; take the blueberry anthocyanin extract and dissolve it in a citric acid-sodium citrate buffer solution with a pH value of 4.0, and prepare 10 g of blueberry anthocyanin extract per 100 ml of buffer solution blueberry anthocyanin solution;

[0041] (2) Glycosidase-catalyzed conversion of blueberry anthocyanins; put the blueberry anthocyanin solution into the enzyme reactor, and add β with an activity of 100 U / mg according to the ratio of blueberry anthocyanin solution: glycosidase = 100 ml: 3g -Glucosidase, activity 200 U / mg α-arabinosidase, activity 200 U / mg β-galactosidase, the temperature is 30 ℃, the stirring speed is 100 rpm, and the enzyme is catalyzed under the conditions of avoiding light The conversion reaction was performed for 2 hours to obtain a blueberry anthocyanin conversion solution; wherei...

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Abstract

The invention relates to a method for preparing high bio-availability blueberry anthocyanin. The method comprises the following operation steps: preparing a blueberry anthocyanin solution, conducting the catalytic conversion of glycosidase of the blueberry anthocyanin, performing the membrane separation on the blueberry anthocyanin conversion product, separating blueberry anthocyanin in a permeate, and drying and preserving the high bio-availability blueberry anthocyanin. The method has the beneficial technical effects that 1) a membrane reactor integrating reaction and separation is adopted to carry out the enzymic catalytic reaction, so that the use efficiency of the enzyme is improved, the production cost is lowered, the reaction product can be discharged continuously, the inhabitation effect of the reaction product on the enzyme is reduced, and even eliminated, and the speed of the catalytic reaction is increased; (2) a membrane reaction system, a reverse osmosis system and an extraction system are integrated to prepare the high bio-availability blueberry anthocyanin which is suitable for industrialization; (3) the bioavailability of the prepared high bio-availability blueberry anthocyanin is higher than 60%, and the high bio-availability blueberry anthocyanin has a relatively high medical health-care value and a relatively wide practical application field.

Description

technical field [0001] The invention belongs to the field of deep processing of food functional components, and in particular relates to a preparation method of blueberry anthocyanins with high bioavailability. Background technique [0002] Blueberry anthocyanin is a flavonoid compound extracted from blueberry (also called lingonberry) fruit with 2-phenylbenzopyran as the core. It consists of delphinidin, cyanidin, paeoniflorin, There are 5 kinds of anthocyanin aglycones in mallow pigment and morning glory anthocyanin, and anthocyanin glycosides formed by linking each anthocyanin aglycon with glucose, galactose, arabinose, acetylglucose, acetylgalactose, and acetylarabinose composition. The antioxidant activity of blueberry anthocyanins is V C 20 times of V E It has physiological functions such as anti-oxidation, anti-aging, anti-virus, anti-cardiovascular disease, promotion of resynthesis of retinoid, and protection of eyesight. For this reason, blueberry anthocyanins a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C07D311/62
Inventor 潘利华贺元康罗建平樊基圣
Owner 江苏沃田集团股份有限公司
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