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Novel green extraction technology for cordyceps polysaccharide

A technology of Cordyceps polysaccharides and Cordyceps, which is applied in the field of microorganisms, can solve the problems of production cost and product quality impact, consumption of alcohol and labor, and low removal rate of monosaccharides, so as to improve mycelium yield, low supporting cost, and simple sugar removal. The effect of high removal rate

Inactive Publication Date: 2014-01-15
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, during the alcohol precipitation process, since monosaccharides are often accompanied by polysaccharides, the removal rate of monosaccharides is low, so multiple alcohol precipitations are required, which consumes more alcohol and labor, and the production cost is higher; at the same time, the traditional thermal Concentration method consumes a lot of steam, which has a great impact on production cost and product quality

Method used

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  • Novel green extraction technology for cordyceps polysaccharide

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Comparison scheme
Effect test

Embodiment 1

[0025] Cordyceps polysaccharide extraction method of the present invention is carried out according to the following steps:

[0026] 1. Source of Cordyceps fermented liquid: provided and identified by China Agricultural Microorganism Culture Collection Management Center, Cordyceps, preservation number ACCC51700. Transfer the strain to the slant medium for conventional culture at 26-28°C for about 30 hours to obtain the activated Cordyceps strain; inoculate the activated Cordyceps strain into the seed medium, wherein the composition of the seed medium is 8% sucrose, 4% yeast powder, 3% peptone, 0.4% potassium dihydrogen phosphate, 0.09% magnesium sulfate, 0.1% defoamer, prepared with water, with natural pH value, shaking culture at 26-28°C for about 30 hours to obtain the first-class seed liquid of Cordyceps; inoculate the first-class seed liquid Enter the secondary seed medium, wherein the secondary seed medium consists of 8% sucrose, 2% yeast powder, 2% peptone, 0.4% potassiu...

Embodiment 2

[0032] Cordyceps polysaccharide extraction method of the present invention, carry out according to the following steps:

[0033] 1, microfiltration filtration: the Cordyceps fermented liquid 7500Kg (fermentation liquid solid content is 3.7%) among the embodiment 1 is filtered by microfiltration, obtains the wet mycelium of the microfiltration dialysate 6372Kg of Cordyceps sinensis and 1128Kg (water content is 76.1%), the light transmittance of the dialysate is 84%, and the wet mycelium can be directly dried and pulverized at low temperature to obtain fermented Cordyceps powder. The membrane material used for microfiltration is a ceramic membrane with a pore size of 100nm. The specific process conditions are: the operating temperature is 50-60°C, the inlet pressure is 2.0bar, the outlet pressure is 1.0bar, and the pressure difference is 1.0bar.

[0034]2. Ultrafiltration filtration: 6372Kg of the microfiltration dialysate obtained in step 1 is filtered by ultrafiltration to obt...

Embodiment 3

[0038] Cordyceps polysaccharide extraction method of the present invention, carry out according to the following steps:

[0039] 1. Microfiltration filtration: 7500Kg of fermented broth (solid content of fermentation broth: 3.7%) fermented by Cordyceps is filtered by microfiltration to obtain 6374Kg of microfiltration dialyzate of Cordyceps and 1126Kg of wet mycelium (water content is 77%) ), the light transmittance of the dialysate is 80%, and the wet mycelium can be directly dried and pulverized at low temperature to obtain fermented Cordyceps fungus powder. The membrane material used for microfiltration is a ceramic membrane with a pore size of 500nm. The specific process conditions are: the operating temperature is 50-60°C, the inlet pressure is 2.3bar, the outlet pressure is 0.9bar, and the pressure difference is 1.4bar.

[0040] 2, ultrafiltration filtration: the microfiltration dialysate 6374Kg obtained in step 1 is filtered by ultrafiltration to obtain ultrafiltration ...

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Abstract

The invention discloses a novel green extraction technology for cordyceps polysaccharide, particularly discloses a method for extracting cordyceps polysaccharide through a two-membrane process. According to the method, the micro-filtration membrane and ultra-filtration membrane treatment and separation are performed on a cordyceps fermentation liquor under specific conditions to obtain the cordyceps polysaccharide. Through the adoption of the method, the traditional extraction mode of water extract and alcohol precipitate of cordyceps polysaccharide is changed, the purification effect of cordyceps polysaccharide is improved, the use level of ethyl alcohol during production is reduced, the economic benefit is increased, meanwhile, the amount of discharged filtrate waste water can be reduced, and the social benefit is obviously improved.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for extracting fungal exopolysaccharides, more precisely, a new method for extracting cordyceps polysaccharides based on membrane technology. Background technique [0002] Cordyceps polysaccharide is a polysaccharide composed of mannose, cordycepin, adenosine, galactose, arabinose, xylose, glucose, and fucose. It is extracted and refined from the fermentation filtrate of Cordyceps using modern bioengineering technology and has physiological activity. The proteoglycan substance is the main active ingredient of Cordyceps sinensis. Experiments have proved that Cordyceps polysaccharide can improve human immune function and increase white blood cells, and it has been used clinically to treat malignant tumors. In addition, Cordyceps sinensis is also used to treat tuberculosis, shortness of breath, wheezing, impotence, nocturnal emission, spontaneous sweating, sor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 王文风徐玲袁兵兵
Owner JIANGSU SHENHUA PHARMA
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