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Production method of bacillus amyloliquefaciens antimicrobial lipopeptide and application thereof in prawn feed

A technology for dissolving starch spores and a production method, applied in the field of aquaculture, can solve the problems of narrow antibacterial spectrum, restricting the large-scale use of feed, etc., and achieve the effects of promoting growth, regulating immunity, improving feed utilization, and reducing mortality.

Inactive Publication Date: 2015-04-08
NANJING AGRICULTURAL UNIVERSITY +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, animal-derived antimicrobial substances have a narrow antibacterial spectrum and usually only inhibit Gram-positive bacteria, which limits their large-scale use in feed

Method used

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  • Production method of bacillus amyloliquefaciens antimicrobial lipopeptide and application thereof in prawn feed
  • Production method of bacillus amyloliquefaciens antimicrobial lipopeptide and application thereof in prawn feed
  • Production method of bacillus amyloliquefaciens antimicrobial lipopeptide and application thereof in prawn feed

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Experimental program
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Effect test

Embodiment 1

[0024] Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) fmb-50 is Bacillus amyloliquefaciens ES-2-4 via N + The high-yielding mutant strains bred by ion mutagenesis, the high-yield mutant strains bred by nitrosoguanidine mutagenesis, and other high-yielding mutant strains obtained by protoplast fusion were named fmb-50. B. amyloliquefaciens fmb-50 can obtain the highest antibacterial lipopeptide production with corn flour as the carbon source, and its production is 3.4 times that of the parent strain ES-2-4. [Yao Shulin, Lu Zhaoxin et al., Taguchi method optimization B. amyloliquefaciens fmb-50 surfactin-producing industrial fermentation medium, Journal of Beijing University of Chemical Technology, 2012,39(4):77-83]. Original strain of fmb-50 B. amyloliquefaciens ES-2-4, B. subtilis 168 and B. subtilis fmbJ (the strains are all known and public, see Huang Xianqing, etc., the effect of Bacillus subtilis fmbJ lipopeptide inhibition point Penicillium and ...

Embodiment 2

[0031] The activated pathogenic bacteria Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Streptococcus agalactiae (the strains are all known and public, see Chen Jixiang et al., Pathogenic Vibrio harveyi hemolytic Gene cloning and detection, 2005, Chinese Fisheries Science; Zhai Xiumei etc. Effects of Vibrio parahaemolyticus on physiological and biochemical indicators of Penaeus vannamei, 2007, Journal of Shanghai Fisheries University; Yan Qingpi et al., Effects of different environmental conditions on the adhesion of Vibrio alginolyticus to intestinal mucus of large yellow croaker, 2006, Acta Aquatica Sinica; Wu Yingrui et al., Research on the inhibitory activity of 153 kinds of Chinese herbal medicines against Streptococcus agalactiae and Streptococcus iniae in tilapia, Journal of Northwest A&F University, 2013) respectively received in TSB (tryptone soybean liquid medium), cultured at 36±1℃ for 4 ~6 h, adjust bacterial OD with TSB medium 600 value, to a ...

Embodiment 3

[0039] Litopenaeus vannamei with normal appearance, healthy body and average tail weight of 1.02 ± 0.10 g were selected as experimental shrimps. Fish meal, soybean meal, peanut meal and brewer's yeast were used as protein sources, fish oil and soybean oil (1:1) as fat source, flour as carbon source, and appropriate minerals and vitamins were added as compound feed for Litopenaeus vannamei (Table 2 ). According to adding 6g (A group), 8g (B group), 10g (C group) described antimicrobial substance feed additive (preparation in the first method in embodiment 1, potency 40000 IU / g) in every kilogram of compound feed, CK group without antibacterial substances (Supplementary Table 2). Three repetitions were set up for each treatment, and 30 Litopenaeus vannamei were stocked in each glass steel bucket, and the water capacity of the glass steel bucket was 0.3 m 3 . The water temperature was (27. 5 ± 1. 5) ℃, the seawater specific gravity was 1. 012 ± 0. 002, and the breeding experi...

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Abstract

The invention relates to a production method of bacillus amyloliquefaciens antibacterial lipopeptide and its application in prawn feed, belonging to the technical field of aquaculture and the field of biotechnology in the agricultural field. Bacillus amyloliquefaciens fmb-50 with the preservation number CGMCC No.6249 is used as the strain, and the fermentation liquid is obtained through seed cultivation, expanded cultivation and fermentation production in sequence; the fermentation liquid is removed by microfiltration or membrane filtration and nanofiltration, Corn starch or porous starch is added for adsorption and spray-dried so that the antibacterial potency of the antibacterial substance feed additive is 40,000-50,000 IU or 250,000 IU / g. The present invention uses Bacillus amyloliquefaciens fmb-50 as raw material to ferment and produce antibacterial substances, which can be produced in a large-scale industrialized manner, has a short production cycle and greatly reduces production costs, and can be used in prawn feed to reduce mortality and improve the feed utilization rate of prawns. Promote non-specific immune response and improve energy metabolism in prawns.

Description

technical field [0001] The invention belongs to the technical field of aquaculture and the field of biotechnology in the agricultural field, and relates to a production method of bacillus amyloliquefaciens antibacterial lipopeptide and its application in prawn feed. Background technique [0002] Antibiotics are used in the aquaculture industry during production and processing, mainly to prevent (preventatives) and treat (therapeutics) bacterial diseases. Adding antibiotics to feed can prevent aquatic diseases, increase aquatic production and save feed. However, the frequent use of antibiotics will lead to the formation of drug resistance in the bacterial flora in the breeding environment. Once the highly pathogenic bacteria acquire drug resistance to the antibiotics shared by humans and animals, the efficacy of the effective antibiotics may be reduced after human infection. Little or even ineffective. In addition, antibiotics left in aquatic products enter the human body a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/18A23K1/17A23K20/195
Inventor 陆兆新赵威钢施雨含吕凤霞别小妹张充
Owner NANJING AGRICULTURAL UNIVERSITY
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