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Preparation method of highly pathogenic porcine reproductive and respiratory syndrome specific transfer factor

A respiratory syndrome, highly pathogenic technology, applied in the direction of peptide preparation methods, chemical instruments and methods, medical preparations containing active ingredients, etc., to reduce morbidity and mortality, convenient preparation methods, and improve immune effects Effect

Inactive Publication Date: 2016-04-20
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no highly pathogenic PRRS-specific TF (PRRS-STF) for clinical need

Method used

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  • Preparation method of highly pathogenic porcine reproductive and respiratory syndrome specific transfer factor
  • Preparation method of highly pathogenic porcine reproductive and respiratory syndrome specific transfer factor
  • Preparation method of highly pathogenic porcine reproductive and respiratory syndrome specific transfer factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) After diluting the highly pathogenic PRRS live vaccine (JXA1-R strain) vaccine with normal saline, intramuscularly inject it into two 3-month-old healthy pigs, immunize once every 2 weeks, and inject 2 copies / head for the first time, The 2nd to 4th immunizations were 4 parts per head; 10 days after the fourth immunization, the blood was collected from the anterior vena cava, and the serum was separated, and the anti-PRRSV antibody level was tested positive by ELISA to qualify. Aseptically adopt the spleen of pigs immunized with porcine reproductive and respiratory syndrome antigen, remove the capsule and connective tissue of the spleen, rinse with normal saline, weigh, and cut into pieces;

[0028] (2) Add 1.5 times of sterile saline according to its weight, grind it with a high-speed tissue grinder at 2500r / min, and make a homogenate;

[0029] (3) Homogenate was frozen at -20°C and thawed at room temperature. After repeated freezing and thawing 8 times, centrifuge ...

Embodiment 2

[0032] (1) After diluting the highly pathogenic PRRS live vaccine (JXA1-R strain) vaccine with normal saline, intramuscularly inject it into two 3-month-old healthy pigs, immunize once every 2 weeks, and inject 2 copies / head for the first time, The 2nd to 4th immunizations were 4 parts per head; 10 days after the fourth immunization, the blood was collected from the anterior vena cava, and the serum was separated, and the anti-PRRSV antibody level was tested positive by ELISA to qualify. Aseptically adopt the spleen of pigs immunized with porcine reproductive and respiratory syndrome antigen, remove the capsule and connective tissue of the spleen, rinse with normal saline, weigh, and cut into pieces;

[0033] (2) Add 3 times of sterile saline according to its weight, grind it with a high-speed tissue grinder at 2500r / min, and make a homogenate;

[0034] (3) Homogenate was frozen at -20°C and thawed at room temperature. After repeated freezing and thawing 8 times, centrifuge at...

Embodiment 3

[0037] (1) After diluting the highly pathogenic PRRS live vaccine (JXA1-R strain) vaccine with normal saline, intramuscularly inject it into two 3-month-old healthy pigs, immunize once every 2 weeks, and inject 2 copies / head for the first time, The 2nd to 4th immunizations were 4 parts per head; 10 days after the fourth immunization, the blood was collected from the anterior vena cava, and the serum was separated, and the anti-PRRSV antibody level was tested positive by ELISA to qualify. Aseptically adopt the spleen of pigs immunized with porcine reproductive and respiratory syndrome antigen, remove the capsule and connective tissue of the spleen, rinse with normal saline, weigh, and cut into pieces;

[0038] (2) Add 2 times the sterile saline according to its weight, grind it with a high-speed tissue grinder at 2500r / min, and make a homogenate;

[0039] (3) Homogenate was frozen at -20°C and thawed at room temperature. After repeated freezing and thawing 8 times, centrifuge at ...

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Abstract

The invention discloses a preparation method of a specific transfer factor for highly pathogenicpathogemc porcine reproductive and respiratory syndrome, and belongs to the technical field of veterinary drugs. The preparation method comprises the following steps of: (1) obtaining splenic organ of a pig subjected to immunization by a highly pathogenicpathogemc porcine reproductive and respiratory syndrome virus antigen in a sterile manner; removing capsula and connective tissue; flushing; cutting into pieces; (2) adding stroke-physiological saline solution; grinding through a high-speed tissue masher to obtain homogenized slurry; (3) freezing and thawing the homogenized slurry repeatedly at eight times; centrifuging for 15 minutes at 4,000r / min under at 4 DEG C; collecting supernate; and (4) transferring the supernate into a dialysis bag to dialyze; collecting external dialysis fluid; filtering; sterilizing; adding a cryoprotectant; mixing; subpackaging; and storing at -20 DEG C. By adopting the specific transfer factor, the immune effect of a porcine reproductive and respiratory syndrome vaccine can be specifically improved, and the immune function and ability of anti-infection of the pig also can be improved or enhanced; and the effects of preventing and treating porcine reproductive and respiratory syndrome and reducing morbidity as well as death rate are brought.

Description

technical field [0001] The invention relates to the technical field of veterinary medicine. Background technique [0002] Porcine reproductive and respiratory syndrome (porcine reproductive and respiratory syndrome, PRRS) is an acute contagious disease of pigs caused by porcine reproductive and respiratory syndrome virus (PRRSV), which is characterized by reproductive disorders and respiratory symptoms. It is a serious hazard to my country's pig industry. one of the most important diseases. PRRSV mainly injures monocytes / macrophages in the body, causing the body's natural and specific immune function to be suppressed. Therefore, PRRSV-infected pigs are prone to secondary pathogens such as streptococcus, swine mycoplasma pneumonia, porcine circovirus type 2, and swine influenza virus. Infection makes the damage more serious. Since 2006, the epidemic has broken out in various parts of my country, and a large number of piglets and sows have become ill and died, causing huge lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/34A61K35/26
Inventor 宋勤叶袁洪兴逯纪成陈桂清孙泰然
Owner HEBEI AGRICULTURAL UNIV.
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